Rhizobial strains exert a major effect on the amino acid composition of alfalfa nodules under NaCl stress

Bertrand, Annick; Bipfubusa, Marie; Dhont, Catherine; Chalifour, François-P.; Drouin, Pascal; Beauchamp, Chantal J.

doi:10.1016/j.plaphy.2016.08.002

Cited by 25 publications

(23 citation statements)

References 53 publications

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“…In addition, Hu et al ( 2014 ) found that plants exposed to salt stress would elicit a broad range of metabolic responses, and various metabolites, such as amino acids, sugars, and organic acids. It has been shown that amino acids, including proline, GABA, glutamine, and arginine among others, played a pivotal role in osmoprotection and was substantially accumulated in salt-stressed plant (Bertrand et al, 2016 ), which is in accordance with our findings. Among those amino acids, proline is considered as a major osmotic regulator, having multiple functions in adjusting osmosis, stabilizing the structure of proteins, and scavenging ROS (Maggio et al, 2002 ; Verbruggen and Hermans, 2008 ).…”

Section: Discussionsupporting

confidence: 93%

The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass

Han

Wang

et al. 2017

Front. Microbiol.

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Perennial ryegrass (Lolium perenne) is an important forage grass with high yield and superior quality in temperate regions which is widely used in parks, sport field, and other places. However, perennial ryegrass is moderately tolerant to salinity stress compared to other commercial cultivars and salt stress reduces their growth and productivity. Aspergillus aculeatus has been documented to participate in alleviating damage induced by salinity. Therefore, the objective of this study was to investigate the mechanisms underlying A. aculeatus-mediated salt tolerance, and forage quality of perennial ryegrass exposed to 0, 200, and 400 mM NaCl concentrations. Physiological markers and forage quality of perennial ryegrass to salt stress were evaluated based on the growth rate, photosynthesis, antioxidant enzymes activity, lipid peroxidation, ionic homeostasis, the nutritional value of forage, and metabolites. Plants inoculated with A. aculeatus exhibited higher relative growth rate (RGR), turf and forage quality under salt stress than un-inoculated plants. Moreover, in inoculated plants, the fungus remarkably improved plant photosynthetic efficiency, reduced the antioxidant enzymes activity (POD and CAT), and attenuated lipid peroxidation (decreased H2O2 and MDA accumulation) induced by salinity, compared to un-inoculated plants. Furthermore, the fungus also acts as an important role in maintaining the lower Na/K ratio and metabolites and lower the amino acids (Alanine, Proline, GABA, and Asparagine), and soluble sugars (Glucose and Fructose) for inoculated plants than un-inoculated ones. Our results suggest that A. aculeatus may be involved in modulating perennial ryegrass tolerance to salinity in various ways.

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Section: Discussionsupporting

confidence: 93%

The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass

Han

Wang

et al. 2017

Front. Microbiol.

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“…Proline has been shown to increase in response to salt tolerance in several plant species and to act as an osmoprotectant [7,36] as well as an ROS scavenger [12,43]. All these results support a previous study comparing different associations of alfalfa cultivars and rhizobium strains showing an increased concentration of compatible carbohydrates and amino acids in response to salinity concurring with a higher WC [9,25]. Thus, the recurrent selection scheme appears to have maintained the mechanisms of salt tolerance inherited from the initial cultivars.…”

Section: Osmoprotectants In Leavessupporting

confidence: 83%

“…We recently confirmed that the use of a salt-tolerant cultivar in symbiosis with a salt-tolerant rhizobium strain increased alfalfa yield under salt stress [9]. More specifically, a previous analysis of the biochemical changes in nodules in response to salinity stress showed that the accumulation of pinitol and sucrose [9], as well as of specific amino acids including proline, glutamate, ornithine, and aspartate [25], was linked to the salinity tolerance of alfalfa-strains associations, warranting further investigation of these specific osmolytes.…”

Section: Introductionsupporting

confidence: 57%

“…Twenty-one amino acids were analyzed: alanine, arginine, asparagine, aspartate, glutamate, glutamine, glycine, γ-aminobutyric acid (GABA), α-aminobutyric acid (AABA), histidine, proline, methionine, lysine, serine, leucine, isoleucine, ornithine, phenylalanine, threonine, tyrosine, and valine. These amino acids were separated and quantified using Waters ACQUITY UPLC analytical system controlled by the Empower II software (WATERS, Milford, MA, USA) as described by [25]. The results from amino acid determinations were expressed as concentrations on a dry matter (DM) basis (µmol g −1 DM).…”

Section: Amino Acid Concentrationsmentioning

confidence: 99%

“…It has, however, been reported that a high salinity level may negatively affect the nodulation capacity by the inhibition of initial steps of the establishment of Rhizobium-legume symbiosis [24,35]. The Sinorhizobium meliloti strain RM1521 has previously been shown to be salt-tolerant and to increase alfalfa capacity to withstand NaCl stress [9,25]. In the current study, we observed that increasing salt stress had little negative impact on root nodulation index, which could be partly explained by the salt tolerance of this rhizobium strain [9].…”

Section: Biomass and Relative Water Contentmentioning

confidence: 99%

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Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

et al. 2020

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Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.

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Selection of rhizobial strains differing in their nodulation kinetics under low temperature in four temperate legume species

et al. 2023

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BackgroundWinter climate change including frequent freeze‐thaw episodes and shallow snow cover will have major impacts on the spring regrowth of perennial crops. Non‐bloating perennial forage legume species including sainfoin, birdsfoot trefoil, red clover, and alsike clover have been bred for their adaptation to harsh winter conditions. In parallel, the selection of cold‐tolerant rhizobial strains could allow earlier symbiotic nitrogen (N) fixation to hasten spring regrowth of legumes.MethodsTo identify strains forming nodules rapidly and showing high N‐fixing potential, 60 rhizobial strains in association with four temperate legume species were evaluated over 11 weeks under spring soil temperatures for kinetics of nodule formation, nitrogenase activity, and host yield.ResultsStrains differed in their capacity to form efficient nodules on legume hosts over time. Strains showing higher nitrogenase activity were arctic strain N10 with sainfoin and strain L2 with birdsfoot trefoil. For clovers, nitrogenase activity was similar for control and inoculated plants, likely due to formation of effective nodules in controls by endophyte rhizobia present in seeds.ConclusionsSelection based on nodulation kinetics at low temperature, nitrogenase activity, and yield was effective to identify performant rhizobial strains for legume crops. The use of cold‐tolerant strains could help mitigate winter climatic changes.

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Rhizobial strains exert a major effect on the amino acid composition of alfalfa nodules under NaCl stress

Cited by 25 publications

References 53 publications

The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass

The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass

Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

Selection of rhizobial strains differing in their nodulation kinetics under low temperature in four temperate legume species

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