Rheology of Penicillium chrysogenum pellet suspensions

Wittler, R.; Matthes, René; Schügerl, K.

doi:10.1007/bf00508124

Cited by 18 publications

(2 citation statements)

References 16 publications

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“…The latter can be induced by manipulation of the growth medium or other environmental factors such as pH. Examples of this have been described for Penicillium chrysogenum and Absidia achylu (Kim et al, 1982;Konig et al, 1982;Wittler et al, 1983). In both cases, increased oxygen mass transfer rates were obtained with the pelleted growth form.…”

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confidence: 99%

Continuous production of penicillin‐g by penicillium chrysogenum cells immobilized on celite biocatalyst support particles

et al. 1986

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Celite R‐630, a commercially available catalyst support, was used successfully in the batch and continuous production of Penicillin‐G by mycelial cells of Penicillium chrysogenum. As an extension of previous work, the productivity of a 1.2 L three phase fluidized bed bioreactor using the Celite support was compared directly with the same reactor in which cells had been immobilized inside a larger carrageenan support matrix. With Celite particles, maximum yields and specific reaction rates for continuous reactor runs were 0.11 g Pen‐G (K+)/g lactose and 0.05 mmol Pen‐G/h/g protein respectively. These results were similar to those obtained with carrageenan beads. However, on a reactor volume basis, Celite was five times more productive than carrageenan. Results are discussed in terms of the potential for using Celite in industrial bioreactor systems.

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confidence: 99%

Continuous production of penicillin‐g by penicillium chrysogenum cells immobilized on celite biocatalyst support particles

et al. 1986

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“…In the past, extensive experimental investigations for the rheological characterisation of pellet‐based cultivations were carried out [15,42,43,103–112]. Although it is important to determine the viscous properties of a cultivation medium in order to estimate the power input of a process, it is not possible to conclude on the mechanical stress of an individual pellet or its influence on the pellet morphology without making major system assumptions [92,113].…”

Section: Experimental Investigations For the Micromechanical Charactementioning

confidence: 99%

Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review

Böl

Schrinner

Tesche

et al. 2020

Engineering in Life Sciences

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Filamentous microorganisms are main producers of organic acids, enzymes, and pharmaceutical agents such as antibiotics and other active pharmaceutical ingredients. With their complex cell morphology, ranging from dispersed mycelia to dense pellets, the cultivation is challenging. In recent years, various techniques for tailor‐made cell morphologies of filamentous microorganisms have been developed to increase product formation and have been summarised under the term morphology engineering. These techniques, namely microparticle‐enhanced cultivation, macroparticle‐enhanced cultivation, and alteration of the osmolality of the culture medium by addition of inorganic salts, the salt‐enhanced cultivation, are presented and discussed in this review. These techniques have already proven to be useful and now await further proof‐of‐concept. Furthermore, the mechanical behaviour of individual pellets is of special interest for a general understanding of pellet mechanics and the productivity of biotechnological processes with filamentous microorganisms. Correlating them with substrate uptake and finally with productivity would be a breakthrough not to be underestimated for the comprehensive characterisation of filamentous systems. So far, this research field is under‐represented. First results on filamentous pellet mechanics are discussed and important future aspects, which the filamentous expert community should deal with, will be presented and critically discussed.

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