Rheological and swelling properties of alginate gels

Segeren, A. J. M.; Boskamp, J. V.; Tempel, M. van den

doi:10.1039/dc9745700255

Cited by 56 publications

(38 citation statements)

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“…2), this being also in agreement with Segeren et al (1974) and Moresi et al (2001). Table 2 Effect of the alginate type (see Table 1) and concentration (c) on the critical strain value (c 0c ) together with the corresponding complex shear modulus (G Ã ) and loss tangent ( In previous work (Moresi et al, 2001), the fourparameter relaxation function used by Friedrich & Heymann (1988), to describe the phase transition of cross-linking networks near the gel point or after the transition sol-gel, was found to be able to reconstruct the evolution of shear storage (G 0 ) and loss (G 00 ) moduli in the frequency domain as follows:…”

Section: Rheometrical Determinations Under Frequency Sweep Testssupporting

confidence: 91%

“…On the contrary, the results of creep experiments showed a liquid-like viscoelastic behaviour that was described by a mechanical model consisting of one Maxwell element in series with two Kelvin-Voigt elements (Mitchell & Blanshard, 1976). In dynamic tests, the mechanical spectra of these gels were found to be characterised by a flat dependency of the shear storage modulus (G 0 ) and loss modulus (G 00 ) on frequency (f ) for f ranging from about 10 À2 to 10 2 s À1 with G 0 one or two orders of magnitude greater than G 00 (Moresi, Mancini, Bruno, & Rancini, 2001;Segeren et al, 1974).…”

Section: Introductionmentioning

confidence: 99%

“…The viscoelastic properties of algal alginate gels have been assessed so far using either static (Mancini, Moresi, & Rancini, 1999a, 1999bMitchell & Blanshard, 1976;Nussinovitch, Peleg, & Normand, 1989) or dynamic (Doublier, Launay, & Cuvelier, 1992;Segeren, Boskamp, & Van Den Tempel, 1974) tests. The results of stress-relaxation exhibited a solid-like behaviour that was described by a mechanical model consisting of two Maxwell elements in parallel with one spring (Mancini et al, 1999a(Mancini et al, , 1999bNussinovitch et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Viscoelastic properties of microbial alginate gels by oscillatory dynamic tests

Moresi¹,

Bruno²,

Parente³

2004

Journal of Food Engineering

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Section: Rheometrical Determinations Under Frequency Sweep Testssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Viscoelastic properties of microbial alginate gels by oscillatory dynamic tests

Moresi¹,

Bruno²,

Parente³

2004

Journal of Food Engineering

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“…Therefore, it is important to not only quantify the mechanical properties of these constructs and scaffolds, but to do so under physiological ionic conditions [25]. Most of the previously-reported mechanical properties of the alginate hydrogel have been obtained in various non-physiological conditions at low-osmotic environment [16,26], therefore, are not directly applicable to tissue engineering for in vivo applications. LeRoux et al [17] was the first to study how the mechanical properties of alginate hydrogel change with the concentration of alginate and storage time of the hydrogel under a physiologically relevant ionic condition (0.15M NaCl and 1.8mM CaCl 2 ).…”

Section: Introductionmentioning

confidence: 99%

“…1). Monovalent ions such as Na + can also compete with calcium ions for junction sites of guluronic residues and thereby weakening the alginate gel [16,17]. The calcium crosslinks can be readily dissolved in the absence of the divalent ions, leading the gel to depolymerize.…”

Section: Introductionmentioning

confidence: 99%

Calcium Concentration Effects on the Mechanical and Biochemical Properties of Chondrocyte-Alginate Constructs

et al. 2008

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Alginate gel crosslinked by calcium ions (Ca 2+ ) has been widely used in cartilage tissue engineering. However, most studies have been largely performed in vitro in medium with a calcium concentration ([Ca 2+ ]) of 1.8mM, while the calcium level in the synovial fluid of the human knee joints, for example, has been reported to be 4mM or even higher. To simulate the synovial environment, the two studies in this paper were designed to investigate how the alginate scaffold alone, as well as the chondrocytes seeded alginate gel responds to variations in medium [Ca 2+ ]. In Study A, the mechanical properties of 2% alginate hydrogel were tested in 0.15M NaCl and various [Ca 2+ ] (1.0mM, 1.8mM, and 4mM). In Study B, primary bovine chondrocytes was seeded in alginate gel, and biochemical contents and mechanical properties were determined after incubation for 28 days in three [Ca 2+ ] (1.8mM, 4mM, and 8mM). For both studies, it was found that the magnitude of the complex shear modulus (|G*|) at 1Hz doubled and the corresponding phase angle shift angle (δ) increased > 2° as a result of the approximate 4-fold change in [Ca 2+ ]. At high [Ca 2+ ], the chondrocyte glycosaminogylcan (GAG) production inside the chondrocyte-alginate constructs was suppressed significantly. This is likely due to a decrease in the porosity of the chondrocyte-alginate constructs as a result of compaction in structure caused by an increased crosslinking density with [Ca 2+ ]. These may be important considerations in the eventual successful implementation of cartilage tissueengineered constructs in the clinical setting.

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Compressive and shear properties of alginate gel: Effects of sodium ions and alginate concentration

LeRoux

Guilak

Setton

1999

J. Biomed. Mater. Res.

373

257

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The equilibrium and viscoelastic properties of alginate gel crosslinked with Ca2+ were determined as a function of alginate concentration and duration of exposure to physiologic concentrations of NaCl. Compressive and shear stress relaxation tests and oscillatory shear tests were performed to measure the material properties at two time periods after storage in NaCl compared to no NaCl exposure. The effect of concentration was determined by testing 1-3% alginate gel in a bath of physiological NaCl and CaCl2. After 15 h of exposure to NaCl, the compressive, equilibrium shear, and dynamic shear moduli decreased by 63, 84, and 90% of control values, respectively. The material properties exhibited no further changes after 7 days of exposure to NaCl. The loss angle and amplitude of the relaxation function in the shear also decreased, indicating less viscous behaviors in both dynamic and transient configurations. All moduli, but not the loss angle, significantly increased with increasing alginate concentration. The observed decrease in compressive and shear stiffness for alginate gel after exposure to Na+ was significant and indicated that physiological conditions will soften the gel over a time period of up to 7 days after gelation. The alginate gel retains significant solid-like behaviors, however, as measured by a loss angle of approximately 3 degrees. This study provides the first available data for material properties of alginate gel tested in physiological saline.

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Rheological and swelling properties of alginate gels

Cited by 56 publications

References 0 publications

Viscoelastic properties of microbial alginate gels by oscillatory dynamic tests

Viscoelastic properties of microbial alginate gels by oscillatory dynamic tests

Calcium Concentration Effects on the Mechanical and Biochemical Properties of Chondrocyte-Alginate Constructs

Compressive and shear properties of alginate gel: Effects of sodium ions and alginate concentration

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