Revisiting Kadenbach: Electron flux rate through cytochrome c‐oxidase determines the ATP‐inhibitory effect and subsequent production of ROS

Vogt, Sebastian; Rhiel, Annika; Weber, Petra; Ramzan, Rabia

doi:10.1002/bies.201600043

Cited by 16 publications

(11 citation statements)

References 147 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When ATP is not consumed, the ATP/ADP ratio rises and respiration slows to state 4. Moreover, the studies by Kadenbach and colleagues have proposed that ATP exerts a negative allosteric modulation of the activity of complex IV . For all these reasons, a lower expenditure of ATP should not increase the amount of mitochondrial ATP.…”

Section: Resultsmentioning

confidence: 99%

A thermal gradient modulates the oxidative metabolism and growth of human keratinocytes

et al. 2017

View full text Add to dashboard Cite

During their spatial and differentiative progression, keratinocytes face a thermal gradient, from 37 °C in the proliferating basal layer to 32 °C found in skin surface. In our study, we hypothesized that this difference in temperature must be balanced by increasing the heat produced during respiratory activity. We demonstrated that at 33 °C human primary keratinocytes and HaCaT cells raised mitochondrial energy metabolism, but not glycolytic activity. At 33 °C, the increased mitochondrial ATP synthesis was associated with a strong induction of the modulator of the respiratory chain estrogen receptor β, whereas uncoupling protein 1 expression was not changed. The enhanced mitochondrial oxidative metabolism was accompanied by a remarkable reduction in proliferation. These results suggest that environmental temperature can modulate the energy metabolism and proliferation of human keratinocytes.

show abstract

Section: Resultsmentioning

confidence: 99%

A thermal gradient modulates the oxidative metabolism and growth of human keratinocytes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…It has been reported that increased generation of ROS requires dynamic change of mitochondrial fission 38 , and Drp1 play a major role in regulating ROS production in apoptosis 39 . Previous studies showed that the induction of the peroxidase activity of Cyt-c is a key event in early apoptosis 40 , while the production of ROS, one of the primary factor of oxidative stress, is determined by Cyt-c-oxidase 41 . In this study, during AT-II cells of PQ intoxication, we detected the ROS production and release of Cyt-c and found that suppression of Drp1 by Mdivi-1 can block ROS generation and Cyt-c release.…”

Section: Discussionmentioning

confidence: 99%

Crosstalk between Mitochondrial Fission and Oxidative Stress in Paraquat-Induced Apoptosis in Mouse Alveolar Type II Cells

Zhao

Cao

et al. 2017

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Paraquat (PQ), as a highly effective and nonselective herbicide, induces cell apoptosis through generation of superoxide anions which forms reactive oxygen species (ROS). Mitochondria, as regulators for cellular redox signaling, have been proved to play an important role in PQ-induced cell apoptosis. This study aimed to evaluate whether and how mitochondrial fission interacts with oxidative stress in PQ-induced apoptosis in mouse alveolar type II (AT-II) cells. Firstly, we demonstrated that PQ promoted apoptosis and release of cytochrome-c (Cyt-c). Furthermore, we showed that PQ broke down mitochondrial network, enhanced the expression of fission-related proteins, increased Drp1 mitochondrial translocation while decreased the expression of fusion-related proteins in AT-II cells. Besides, inhibiting mitochondrial fission using mdivi-1, a selective inhibitor of Drp1, markedly attenuated PQ-induced apoptosis, release of Cyt-c and the generation of ROS. These results indicate that mitochondrial fission involves in PQ-induced apoptosis. Further study demonstrated that antioxidant ascorbic acid inhibited Drp1 mitochondrial translocation, mitochondrial fission and attenuated PQ-induced apoptosis. Overall, our findings suggest that mitochondrial fission interplays with ROS in PQ-induced apoptosis in mouse AT-II cells and mitochondrial fission could serve as a potential therapeutic target in PQ poisoning.

show abstract

“…This mechanism guarantees optimal ATP synthesis, moderate oxygen uptake and low generation of reactive oxygen species (ROS) [ 5 ] and is stated as the “second mechanism of respiratory control” [ 6 ]. Although different mechanisms regulate the function of CytOx [ 7 , 8 ], the inhibition of CytOx by ATP occurring at very high ATP/ADP ratios [ 9 ] is allosterically controlled by the dimeric conformation [ 3 ]. However, to work with the isolated enzyme requires the enzymatic preparation with cholate [ 10 , 11 ] that actually induces a stable dimerization of CytOx itself [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Cholate Disrupts Regulatory Functions of Cytochrome c Oxidase

Ramzan

Napiwotzki²,

Weber

et al. 2021

Cells

Self Cite

View full text Add to dashboard Cite

Cytochrome c oxidase (CytOx), the oxygen-accepting and rate-limiting enzyme of mitochondrial respiration, binds with 10 molecules of ADP, 7 of which are exchanged by ATP at high ATP/ADP-ratios. These bound ATP and ADP can be exchanged by cholate, which is generally used for the purification of CytOx. Many crystal structures of isolated CytOx were performed with the enzyme isolated from mitochondria using sodium cholate as a detergent. Cholate, however, dimerizes the enzyme isolated in non-ionic detergents and induces a structural change as evident from a spectral change. Consequently, it turns off the “allosteric ATP-inhibition of CytOx”, which is reversibly switched on under relaxed conditions via cAMP-dependent phosphorylation and keeps the membrane potential and ROS formation in mitochondria at low levels. This cholate effect gives an insight into the structural-functional relationship of the enzyme with respect to ATP inhibition and its role in mitochondrial respiration and energy production.

show abstract

Revisiting Kadenbach: Electron flux rate through cytochrome c‐oxidase determines the ATP‐inhibitory effect and subsequent production of ROS

Cited by 16 publications

References 147 publications

A thermal gradient modulates the oxidative metabolism and growth of human keratinocytes

A thermal gradient modulates the oxidative metabolism and growth of human keratinocytes

Crosstalk between Mitochondrial Fission and Oxidative Stress in Paraquat-Induced Apoptosis in Mouse Alveolar Type II Cells

Cholate Disrupts Regulatory Functions of Cytochrome c Oxidase

Contact Info

Product

Resources

About