Revision of the genus Massilia La Scola et al. 2000, with an emended description of the genus and inclusion of all species of the genus Naxibacter as new combinations, and proposal of Massilia consociata sp. nov.

Abstract

A Gram-stain-negative, rod-shaped, non-spore-forming bacterium originating from a human clinical specimen was studied for its taxonomic position. 16S rRNA gene sequence similarity studies clearly allocated this strain (CCUG 58010T) to the class Betaproteobacteria, closely related to members of the genera Massilia and Naxibacter. Naxibacter varians was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (97.5 %), followed by Massilia niastensis (96.8 %) and Massilia aerilata (96.4 %). Similarities to all other species of the genera Naxibacter and Massilia were in the range 93.9–96.2 %. Chemotaxonomic data (major ubiquinone: Q-8; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; and major fatty acids: summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1ω7c and C12 : 0, with C10 : 0 3-OH as hydroxylated fatty acid) supported the affiliation of the isolate to these genera, which share these chemotaxonomic traits. DNA–DNA hybridization of strain CCUG 58010T with the type strain of N. varians CCUG 35299T resulted in a relatedness value of 39.2 % (reciprocal, 50 %) and physiological and biochemical tests also allowed phenotypic differentiation of the isolate from the most closely related species. There is currently no justification for a division of the genera Massilia and Naxibacter and for this reason a proposal is made to transfer all species of the genus Naxibacter to the genus Massilia, as Massilia alkalitolerans comb. nov., Massilia varians comb. nov., Massilia haematophila comb. nov. and Massilia suwonensis comb. nov. Strain CCUG 58010T represents a novel species, for which the name Massilia consociata sp. nov. is proposed, with the type strain CCUG 58010T ( = CCM 7792T).

Massilia chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from soil

“…2). The strain TA-C7e T was found to have phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol as major polar lipids, which is in accordance with the emended description of the genus Massilia (Kämpfer et al 2011). Additionally, strain TA-C7e T was observed to have an unidentified aminophospholipid as a major polar lipid that was detected as a major compound in M. aurea AP13 T and moderate to minor amounts in M. dura CCUG 52213 T , M. norwichensis NS9 T , M. plicata DSM 17505 T , and M. timonae CCUG 45783 T .…”

Draft Genome Sequence of Massilia sp. Strain BSC265, Isolated from Biological Soil Crust of Moab, Utah

“…16S ribosomal analysis showed 99% identity to the 16S sequence of Massilia consociata strain CCUG 58010 from the NCBI 16S Ribosomal RNA Sequences Database using BLAST (9). The G+C content was 65.4%, which is consistent with the Massilia genus (10). …”

Massilia arenosa sp. nov., isolated from the soil of a cultivated maize field

“…Since the incorporation of the genus Massilia, many additional species have been added and, with its 41 named and described species, Massilia is the most species-rich genus of the Oxalobacteraceae [8]. Since 2005, the genus Massilia now also includes the species of the genus Naxibacter that were first added to the family Oxalobacteraceae [9], but later reclassified based on 16S rRNA gene sequence and biochemical data [10]. The genus Massilia is most closely related to the genera Telluria and Duganella [11].…”