Review on Fatty Acid Desaturases and their Roles in Temperature Acclimatisation

Garba, Lawal; Mo, Mohd Shukuri; Os, Siti Nurbaya; Zalih, Raja Noor

doi:10.3923/jas.2017.282.295

Cited by 26 publications

(13 citation statements)

References 66 publications

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“…Here, the ∆9-des gene was amplified in all Vibrio sp. isolates, suggesting its potential role for the adaptation of cell membranes to fluctuating environmental conditions in this genus [57]. Finally, although both Vibrio sp.…”

Section: Discussionmentioning

confidence: 88%

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

et al. 2020

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Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), such as eicosapentaenoic acid (EPA) (20:5n-3) and docosahexaenoic acid (DHA) (22:6n-3), are considered essential for human health. Microorganisms are the primary producers of omega-3 fatty acids in marine ecosystems, representing a sustainable source of these lipids, as an alternative to the fish industry. Some marine bacteria can produce LC-PUFAs de novo via the Polyunsaturated Fatty Acid (Pfa) synthase/ Polyketide Synthase (PKS) pathway, which does not require desaturation and elongation of saturated fatty acids. Cultivation-independent surveys have revealed that the diversity of microorganisms harboring a molecular marker of the pfa gene cluster (i.e., pfaA-KS domain) is high and their potential distribution in marine systems is widespread, from surface seawater to sediments. However, the isolation of PUFA producers from marine waters has been typically restricted to deep or cold environments. Here, we report a phenotypic and genotypic screening for the identification of omega-3 fatty acid producers in free-living bacterial strains isolated from 5, 500, and 1000 m deep coastal seawater from the Bay of Biscay (Spain). We further measured EPA production in pelagic Vibrio sp. strains collected at the three different depths. Vibrio sp. EPA-producers and non-producers were simultaneously isolated from the same water samples and shared a high percentage of identity in their 16S rRNA genes, supporting the view that the pfa gene cluster can be horizontally transferred. Within a cluster of EPA-producers, we found intraspecific variation in the levels of EPA synthesis for isolates harboring different genetic variants of the pfaA-KS domain. The maximum production of EPA was found in a Vibrio sp. strain isolated from a 1000 m depth (average 4.29% ± 1.07 of total fatty acids at 10 °C, without any optimization of culturing conditions).

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Section: Discussionmentioning

confidence: 88%

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

et al. 2020

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“…For the formation of unsaturated fatty acids (C16:1, C18:1, and C18:2) a fatty acid desaturase is required [ 33 ]. A single gene, g3345.t1, was predicted to encode this enzyme in C. oleaginosus ATCC 20509.…”

Section: Resultsmentioning

confidence: 99%

Genome-scale metabolic modeling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production

Pham

Reijnders

Suárez-Diez

et al. 2021

Biotechnol Biofuels

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Background Cutaneotrichosporon oleaginosus ATCC 20509 is a fast-growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. Results To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon-to-nitrogen (C/N) ratio in the media. This condition-specific biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose, and glycerol as sole carbon source. Conclusion Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can effectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and fine tune a cost-effective production process. Our model suggests ATP-citrate lyase as a possible target to further improve lipid production.

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“…In 12 h incubation regimen, UL level was slightly affected by strain ratio, with 75:25 combinations leading to highest interference with UL increase, indicating that BGHN14 may be responsible for observed effects. Consumption of oxygen by lactobacilli or eventual sequestration of cofactors needed for desaturase activity, are some of the reasons that might have contributed to the reduction of enzyme activity after lactobacilli addition [60, 61]. Furthermore, some lactobacilli strains were shown to increase the oxidative deterioration of lipids in meat products, via hydrogen peroxide production [62].…”

Section: Resultsmentioning

confidence: 99%

Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed

et al. 2019

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The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.

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Review on Fatty Acid Desaturases and their Roles in Temperature Acclimatisation

Cited by 26 publications

References 66 publications

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

Genome-scale metabolic modeling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production

Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed

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