Review of Literature on Akkermansia muciniphila and its Possible Role in the Etiopathogenesis and Therapy of Type 2 Diabetes Mellitus

Sanjiwani, Made Indira Dianti; Aryadi, I Putu Hendri; Semadi, I Made Siswadi

doi:10.15605/jafes.037.01.13

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…Besides, other researchers have established that the genera Ruminococcus was positively correlated with type 2 diabetes, while Bifidobacterium and Akkermansia were negatively associated with type 2 diabetes [50], thus explaining the metagenomic results that we have obtained. Moreover, other studies support these affirmations and also confer glucose-lowering properties upon Akkermansia, Bifidobacterium and Lactobacillus [50,58,[65][66][67].…”

Section: Discussionmentioning

confidence: 65%

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Yavorov-Dayliev,

Milagro,

Ayo

et al. 2023

Diabetologia

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Aims/hypothesis Modulation of gut microbiota has emerged as a promising strategy to treat or prevent the development of different metabolic diseases, including type 2 diabetes and obesity. Previous data from our group suggest that the strain Pediococcus acidilactici CECT9879 (pA1c) could be an effective probiotic for regulating glucose metabolism. Hence, the objectives of this study were to verify the effectiveness of pA1c on glycaemic regulation in diet-induced obese mice and to evaluate whether the combination of pA1c with other normoglycaemic ingredients, such as chromium picolinate (PC) and oat β-glucans (BGC), could increase the efficacy of this probiotic on the regulation of glucose and lipid metabolism. Methods Caenorhabditis elegans was used as a screening model to describe the potential synbiotic activities, together with the underlying mechanisms of action. In addition, 4-week-old male C57BL/6J mice were fed with a high-fat/high-sucrose diet (HFS) for 6 weeks to induce hyperglycaemia and obesity. Mice were then divided into eight groups (n=12 mice/group) according to dietary supplementation: control-diet group; HFS group; pA1c group (1010 colony-forming units/day); PC; BGC; pA1c+PC+BGC; pA1c+PC; and pA1c+BGC. Supplementations were maintained for 10 weeks. Fasting blood glucose was determined and an IPGTT was performed prior to euthanasia. Fat depots, liver and other organs were weighed, and serum biochemical variables were analysed. Gene expression analyses were conducted by real-time quantitative PCR. Sequencing of the V3–V4 region of the 16S rRNA gene from faecal samples of each group was performed, and differential abundance for family, genera and species was analysed by ALDEx2R package. Results Supplementation with the synbiotic (pA1c+PC+BGC) counteracted the effect of the high glucose by modulating the insulin–IGF-1 signalling pathway in C. elegans, through the reversal of the glucose nuclear localisation of daf-16. In diet-induced obese mice, all groups supplemented with the probiotic significantly ameliorated glucose tolerance after an IPGTT, demonstrating the glycaemia-regulating effect of pA1c. Further, mice supplemented with pA1c+PC+BGC exhibited lower fasting blood glucose, a reduced proportion of visceral adiposity and a higher proportion of muscle tissue, together with an improvement in the brown adipose tissue in comparison with the HFS group. Besides, the effect of the HFS diet on steatosis and liver damage was normalised by the synbiotic. Gene expression analyses demonstrated that the synbiotic activity was mediated not only by modulation of the insulin–IGF-1 signalling pathway, through the overexpression of GLUT-1 and GLUT-4 mediators, but also by a decreased expression of proinflammatory cytokines such as monocyte chemotactic protein-1. 16S metagenomics demonstrated that the synbiotic combinations allowed an increase in the concentration of P. acidilactici, together with improvements in the intestinal microbiota such as a reduction in Prevotella and an increase in Akkermansia muciniphila. Conclusions/interpretation Our data suggest that the combination of pA1c with PC and BGC could be a potential synbiotic for blood glucose regulation and may help to fight insulin resistance, diabetes and obesity. Graphical Abstract

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Section: Discussionmentioning

confidence: 65%

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Yavorov-Dayliev,

Milagro,

Ayo

et al. 2023

Diabetologia

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“…To explore the effects of CDs on glucose metabolism and gut microbiota, we established a mouse model of chronic CD exposure and added a probiotic supplementation group for comparison. Accumulating evidence reveals that several intestinal bacteria are beneficial for improving T2DM, such as Bifidobacterium , Lactobacillus , Bacillus , Streptococcus thermophilus , and Akkermansia , which can be used as probiotics. − In this study, VSL#3 was selected for probiotic supplementation, which has been extensively studied and tested in animal models of intestinal inflammation and contains a high concentration (450 billion colonies/sachet) mixture of viable, lyophilized Bifidobacterium , Lactobacillus , and Bacillus thermophilus . , Mice treated with CDs are shown in Figure a. After 14 weeks of CD exposure, the mice showed high fasting glucose levels (Figure b), unchanged fasting weight (Figure c), and impaired glucose tolerance (Figure d and e), indicating disturbed glucose homeostasis.…”

Section: Resultsmentioning

confidence: 99%

Foodborne Carbon Dot Exposure Induces Insulin Resistance through Gut Microbiota Dysbiosis and Damaged Intestinal Mucus Layer

Zhang

Fan

et al. 2023

ACS Nano

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Foodborne carbon dots (CDs), an emerging food nanocontaminant, are an increasing risk factor for metabolic toxicity in mammals. Here, we report that chronic CD exposure induced glucose metabolism disorders via disruption of the gut−liver axis in mice. 16s rRNA analysis demonstrated that CD exposure decreased the abundance of beneficial bacteria (Bacteroides, Coprococcus, and S24-7) and increased the abundance of harmful bacteria (Proteobacteria, Oscillospira, Desulfovibrionaceae, and Ruminococcaceae), as well as increased the Firmicutes/Bacteroidetes ratio. Mechanistically, the increased pro-inflammatory bacteria release the endotoxin lipopolysaccharide, which induces an intestinal inflammation and disruption of the intestinal mucus layer, activating systemic inflammation and inducing hepatic insulin resistance in mice via the TLR4/NFκB/MAPK signaling pathway. Furthermore, these changes were almost completely reversed by probiotics. Fecal microbiota transplantation from CD-exposed mice induced glucose intolerance, damaged liver function, intestinal mucus layer injury, hepatic inflammation, and insulin resistance in the recipient mice. However, microbiota-depleted mice exposed to CDs had normal levels of these biomarkers consistent with microbiota-depleted control mice, which revealed that gut microbiota dysbiosis contributes to CDinduced inflammation-mediated insulin resistance. Together, our findings revealed that gut microbiota dysbiosis contributes to CD-induced inflammation-mediated insulin resistance and attempted to elucidate the specific underlying mechanism. Furthermore, we emphasized the importance of assessing the hazards associated with foodborne CDs.

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“…FOS addition could increase the abundance of A. muciniphila that had been previously reported associated with insulin resistance as evidenced by HOMA-IR value and glucose AUC in glucose tolerance test (GTT). A. muciniphila has been increasingly recognized to reduce insulin resistance in different tissues and improve blood glucose balance ( Sanjiwani et al., 2022 ; Zhang et al., 2021 ). The placenta is a key bridge between mother and fetus and plays an important role in fetal development ( Hu et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Maternal or post-weaning dietary fructo-oligosaccharide supplementation reduces stillbirth rate of sows and diarrhea of weaned piglets

Ma,

Su,

et al. 2024

Animal Nutrition

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Review of Literature on Akkermansia muciniphila and its Possible Role in the Etiopathogenesis and Therapy of Type 2 Diabetes Mellitus

Cited by 8 publications

References 42 publications

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Foodborne Carbon Dot Exposure Induces Insulin Resistance through Gut Microbiota Dysbiosis and Damaged Intestinal Mucus Layer

Maternal or post-weaning dietary fructo-oligosaccharide supplementation reduces stillbirth rate of sows and diarrhea of weaned piglets

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