Review of genetic studies of susceptibility to facial eczema in sheep and dairy cattle

Morris, C. A.; Phua, S. H.; Cullen, N. G.; Towers, N. R.

doi:10.1080/00288233.2012.757240

Cited by 5 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite a large amount of FE research since the first report in sheep in 1897 (Lancashire and Keogh 1968 ) comparatively little has focused on the disease in cattle. Publications have concentrated on case reports from natural challenges, and on the genetic susceptibility of Friesian and Jersey cattle (Morris et al 1991a , b , 1998 , 2013 ). Furthermore, no data has been published on FE research using the modern techniques of metabolomics.…”

Section: Introductionmentioning

confidence: 99%

“…Subclinically affected animals show no skin abnormalities, but liver damage can be revealed by blood biochemistry tests. Measurement of the serum activity of γ-glutamyltransferase (GGT) and glutamate dehydrogenase (GDH), enzymes which indicate bile duct and liver cell damage, respectively, have been used for many years to identify subclinical and clinical cases of FE (Morris et al 2004 , 2013 ; Phua et al 2009 ). The activity of GGT, however, shows a lag phase of 10–14 days, before increasing (Bennison et al 2010 ; Di Menna et al 2009 ; Morris et al 1998 ; Smith and Gravett 1986 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serum metabolomics using ultra performance liquid chromatography coupled to mass spectrometry in lactating dairy cows following a single dose of sporidesmin

et al. 2018

View full text Add to dashboard Cite

IntroductionPhotosensitization is a common clinical sign in cows suffering from liver damage caused by the mycotoxin sporidesmin. This disease, called facial eczema (FE), is of major importance in New Zealand. Current techniques for diagnosing animals with subclinical sporidesmin-induced liver damage (i.e. without photosensitization) are nonspecific. In addition, little is known of the mechanisms involved in sporidesmin resistance, nor the early effects seen following low-dose sporidesmin intoxication.ObjectiveThe objective of this study was to identify individual metabolites or metabolic profiles that could be used as serum markers for early stage FE in lactating cows.MethodsResults are presented from a 59-day sporidesmin challenge in Friesian-cross dairy cows. Serum metabolite profiles were obtained using reversed phase ultra-performance liquid chromatography (UPLC) electrospray ionization mass spectrometry (MS) and UPLC tandem MS. Multivariate and time series analyses were used to assess the data.ResultsStatistical analysis, both with and without the temporal component, could distinguish the profiles of animals with clinical signs from the others, but not those affected subclinically. An increase in the concentrations of a combination of taurine- and glycine-conjugated secondary bile acids (BAs) was the most likely cause of the separation. This is the first time that MS methods have been applied to FE and that bile acids changes have been detected in cattle exposed to sporidesmin.ConclusionsIt is well known that BA concentrations increase during cholestasis due to damage to bile ducts and leakage of the bile. This is the first study to investigate metabolomic changes in serum following a sporidesmin challenge. Further work to establish the significance of the elevation of individual BAs concentrations in the serum of early-stage sporidesmin-poisoned cows is necessary.Electronic supplementary materialThe online version of this article (10.1007/s11306-018-1358-4) contains supplementary material, which is available to authorized users.

show abstract