Review: Factors affecting mass propagation of Vanda orchid in vitro

Setiaji, Arkan; Annisa, Rr. Rifka; Santoso, Agung Dwi; Kinasih, Anggiresti; Riyadi, Anindita Dr.

doi:10.13057/cellbioldev/v050201

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Bihar and the western peninsula of India are the only places it may be found. Many species of Vanda are extensively used in traditional and folk medical systems in India, Nepal, China, and other Asian countries 16 . When it comes to traditional Indian medicine, Vanda tessellata is known as "Rasna" in Ayurveda, which is known as the most commonly used medicinal orchid in the genus.…”

Section: Traditional Uses Of Orchids Speciesmentioning

confidence: 99%

Untitled

2022

IJPSR

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In order to discover new treatments for a wide range of ailments, ethnopharmacological research has investigated different leads from plants. In nature, the Orchidaceae family's Vanda roxburghii is found in Bangladesh and India. As a remedy for dyspepsia, bronchitis, and inflammations, as well as for rheumatoid arthritis and bronchitis, various portions of the plant are employed. A number of the plant's beneficial compounds have been identified, including alkaloids, glucosides, fatty acids, saponin, and tannin compounds, as well as β-and γ-sitosterols, as well as fatty oils, resin, and tetracosylferrulate in the roots. In the angiosperm family, orchids make up the largest and most diversified group. They are grown for their magnificent blooms. Flowers of all sizes, shapes and colors can be found on these plants. However, these plants have been widely recognized for their economic relevance, but little attention has been paid to their medical properties. Ayurvedic practitioners have long relied on orchids to treat a wide range of diseases. For centuries, people have relied on the herb to treat a variety of diseases. Numerous biological activities have yielded positive benefits for the plant. This plant has aphrodisiac, antifungal, antiulcer, anticonvulsant, and antioxidant properties. Other studies have shown that the plant possesses anti-inflammatory, wound-healing and analgesic properties, as well as anti-diabetic properties. The phytochemistry and biological activities of this important medicinal plant are at the center of the key implications. INTRODUCTION:Vanda roxburghii (Synonym Vanda tessellate Roxb.), belonging to the family Orchidaceae, is an epiphytic perennial orchid native to the Indian subcontinent Roxb. A foliagerich stem that is sturdy and gives rise to several thick and juicy roots.

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Section: Traditional Uses Of Orchids Speciesmentioning

confidence: 99%

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2022

IJPSR

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“…The limited cultivation of V. limbata is caused by its very long juvenile period or slow growth. Setiaji et al (2021) stated that Vanda orchid cultivation takes more than 3 years from juvenile to flowering. If this condition continues, it is feared that it will have an impact on the lack of orchid populations in nature.…”

Section: Introductionmentioning

confidence: 99%

Effect of Benzyl Amino Purine (BAP) on the Leaf Growth of Vanda limbata Blume Orchid In Vivo

Az Zahra,

Setiari,

Nurchayati

2024

Caraka Tani J. Sustain. Agric.

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Vanda limbata is a natural orchid with slow growth and is often taken directly from the forest in large quantities, threatening its sustainability. Ex-situ preservation efforts are needed for this orchid, one of which is through cultivation with the addition of Benzyl Amino Purin (BAP), known to increase shoot growth. This research aims to analyze the effect of BAP and determine its optimal concentration on the growth of V. limbata orchids. This research used a single-factor completely randomized design in BAP concentrations of 0, 25 and 50 mg l-1 with 5 replications. The method used was spraying BAP solution once a week on all parts of adult V. limbata orchids aged ±2 years. The research was conducted for 3 months in the experimental garden with parameters observed in the form of the number of new leaves, elongation of old leaves, the length of new leaves, increase in the width of old leaves, the width of new leaves, stomatal density and the number of roots. The results showed that the most effective BAP concentration was 50 mg l-1 for the number of new leaves which increased by 200%, the length of old leaves which increased by 168.66% and the length of new leaves which increased by 800%, but resulted in less stomatal density than the control. BAP can increase the growth of V. limbata orchid leaves. This research can be a recommendation for ex-situ conservation efforts that have the potential to be developed as a means of orchid cultivation.

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“…In vitro propagation with seed as propagules is an effective method for orchid conservation since it increases the orchid's germination rate, provides massive amounts of healthy plants with diverse genotypes, and can be done in all seasons throughout the year [8]. This particular method requires external factors such as light and medium composition because orchid germination is a complicated process [9]. Several orchid species need light for their germination, they are categorized as photoblastic plants while other species are negative photoblastic plants because they do not require light.…”

Section: Introductionmentioning

confidence: 99%

“…Both MS and VW media are expensive therefore modified medium from 1255 (2023) 012026 IOP Publishing doi:10.1088/1755-1315/1255/1/012026 2 natural organic extract and foliar fertilizer can be an alternative option. Frequently-used organic extract are banana, tomato, and coconut water [9].…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

Gayatri,

Astarini,

Pharmawati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Vanda tricolor is an endemic orchid from Indonesia that can be found in Bali. As part of Orchidaceae, Vanda tricolor is facing the same problem as other orchid species, their seeds have various requirements for germination which causes a low germination rate. Preventing this endangered orchid from extinction with tissue culture not only maintains the genetic diversity but also understanding the growth factors of this very species. The aim of this research was to analyze how sterilization, medium, and light affect the germination of V. tricolor. Wild V. tricolor pod from Buahan Village, Kintamani, Bangli Regency, Bali, was sterilized with 2g/L fungicide, 1,05% and 0,79% NaClO; while seeds were sterilized with 1,05% NaClO. The seeds from these two different sterilization methods were cultured on Murashige and Skoog (MS) medium, Vacin and Went (VW) medium, Organic 1 (O1) medium, and Organic 2 (O2) medium, then half of them were placed under LED light and the rest were kept on dark. Each one of the sixteen treatment combinations was replicated six times. Seed sterilization with 1,05% NaClO showed better results than pod sterilization. Seeds started to germinate 3 weeks after sown with the highest germination rate occurred on seeds cultured on MS medium under LED light. This study revealed tissue culture with different treatments will aid to understand the preferred germination condition for wild Vanda tricolor and support its conservation.

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Review: Factors affecting mass propagation of Vanda orchid in vitro

Cited by 7 publications

References 38 publications

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Effect of Benzyl Amino Purine (BAP) on the Leaf Growth of Vanda limbata Blume Orchid In Vivo

In vitro culture of wild Vanda tricolor Lindley seeds from Kintamani, Bali, as initial step for conservation

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