Reversible conformational changes in the parallel type G-quadruplex structure inside a thermoresponsive hydrogel

Hasuike, Erika; Akimoto, Aya Mizutani; Kuroda, Reiko; Matsukawa, Ko; Hiruta, Yuki; Kanazawa, Hideko; Yoshida, Ryo

doi:10.1039/c7cc00279c

Cited by 23 publications

(9 citation statements)

References 25 publications

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“…This example illustrates how quadruplex formation can affect the properties of hydrogels, but the inverse approach is possible as well: hydrogels can affect the properties of quadruplexes. Hasuike et al took advantage of the space inside thermoresponsive poly( N -isopropylacrylamide) gels to control the stability of G-quadruplex reversibly via variations in temperature (25–45 °C) . This could find applications for the controlled denaturation of an aptamer, releasing its protein cargo upon a change in temperature.…”

Section: Nanocarriers and Therapeuticsmentioning

confidence: 99%

DNA Quadruple Helices in Nanotechnology

2019

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DNA has played an early and powerful role in the development of bottomup nanotechnologies, not least because of DNA's precise, predictable, and controllable properties of assembly on the nanometer scale. Watson−Crick complementarity has been used to build complex 2D and 3D architectures and design a number of nanometer-scale systems for molecular computing, transport, motors, and biosensing applications. Most of such devices are built with classical B-DNA helices and involve classical A-T/U and G-C base pairs. However, in addition to the above components underlying the iconic double helix, a number of alternative pairing schemes of nucleobases are known. This review focuses on two of these noncanonical classes of DNA helices: G-quadruplexes and the imotif. The unique properties of these two classes of DNA helix have been utilized toward some remarkable constructions and applications: G-wires; nanostructures such as DNA origami; reconfigurable structures and nanodevices; the formation and utilization of hemin-utilizing DNAzymes, capable of generating varied outputs from biosensing nanostructures; composite nanostructures made up of DNA as well as inorganic materials; and the construction of nanocarriers that show promise for the therapeutics of diseases.

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Section: Nanocarriers and Therapeuticsmentioning

confidence: 99%

DNA Quadruple Helices in Nanotechnology

2019

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“…Further diversity is provided by the ability of intercalating small molecules into the grooves of dsDNA, based on a combination of π–π stacking interactions with the DNA base pairs as well as electrostatic interactions with the negatively charged DNA backbone . Previously, several groups have developed thermoresponsive DNA by attaching a thermoresponsive polymer to the helical backbone of DNA, either covalently or by intercalation of a thermoresponsive polymer featuring a DNA intercalator as end‐group . However, these thermoresponsive DNA constructs rely on the thermoresponsivity of the attached polymers and this approach does not render the stiff and helical dsDNA strand itself thermoresponsive.…”

Section: Methodsmentioning

confidence: 99%

Thermoresponsive DNA by Intercalation of dsDNA with Oligoethylene‐Glycol‐Functionalized Small‐Molecule Intercalators

Bera

Verdonck

Glaßner

et al. 2019

Macromol. Rapid Commun.

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Thermoresponsive polymeric materials are important building blocks for smart materials. In this work, the transformation of dsDNA into a thermoresponsive polymer is reported by intercalation of short, oligoethylene‐glycol‐modified proflavine intercalators. The thermoresponsiveness of the dsDNA‐intercalator complex originates from the heating‐induced dehydration of the ethylene glycol side chains, which leads to aggregation of the intercalated dsDNA. This work demonstrates the possibility of designing small‐molecule intercalators to prepare thermoresponsive dsDNA complexes with tunable lower critical solution temperature behavior.

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“…DNA‐PEG‐DNA triblock conjugates, in which merely four or five consecutive nucleotides are symmetrically introduced, were prepared at least on a gram scale applying a solution‐phase large scale DNA synthesis, thus realizing intelligent and biodegradable hydrogels (DNA quadruplex gels). By utilizing efficient formation of G‐quadruplexes as the crosslinking points, DNA quadruplex gels shows sol‐to‐gel (or gel‐to‐sol) transition responsive to various input signals, such as Na + , K + , and a complementary DNA strand. DNA quadruplex gels can be instantly prepared and even show self‐healing properties.…”

Section: Figurementioning

confidence: 99%

Intelligent, Biodegradable, and Self‐Healing Hydrogels Utilizing DNA Quadruplexes

Tanaka

Wakabayashi

Fukushima

et al. 2017

Chemistry An Asian Journal

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A new class of hydrogels utilizing DNA (DNA quadruplex gel) has been constructed by directly and symmetrically coupling deoxynucleotide phosphoramidite monomers to the ends of polyethylene glycols (PEGs) in liquid phase, and using the resulting DNA‐PEG‐DNA triblock copolymers as macromonomers. Elongation of merely four deoxyguanosine residues on PEG, which produces typically ≈10 grams of desired DNA‐PEG conjugates in one synthesis, resulted in intelligent and biodegradable hydrogels utilizing DNA quadruplex formation, which are responsive to various input signals such as Na+, K+, and complementary DNA strand. Gelation of DNA quadruplex gels takes place within a few seconds upon the addition of a trigger, enabling free formation just like Ca+‐alginate hydrogels or possible application as an injectable polymer (IP) gel. The obtained hydrogels show good thermal stability and rheological properties, and even display self‐healing ability.

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Reversible conformational changes in the parallel type G-quadruplex structure inside a thermoresponsive hydrogel

Cited by 23 publications

References 25 publications

DNA Quadruple Helices in Nanotechnology

DNA Quadruple Helices in Nanotechnology

Thermoresponsive DNA by Intercalation of dsDNA with Oligoethylene‐Glycol‐Functionalized Small‐Molecule Intercalators

Intelligent, Biodegradable, and Self‐Healing Hydrogels Utilizing DNA Quadruplexes

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