Rev1 wbdR tagged vaccines against Brucella ovis

Aragón-Aranda, Beatriz; Miguel, María Jesús de; Martínez-Gómez, Estrella; Zúñiga-Ripa, Amaia; Salvador-Bescós, Miriam; Moriyón, Ignacio; Iriarte, Maite; Muñoz, Pilar M.; Conde-Álvarez, Raquel

doi:10.1186/s13567-019-0714-3

Cited by 10 publications

(7 citation statements)

References 33 publications

(53 reference statements)

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“…[14,15]. Vaccination and treatment of brucellosis in farm animals are not considered 100% safe for human health, hence are forbidden in many countries [7,[16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Jamil

Melzer

Saqib

et al. 2020

IJERPH

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Bovine brucellosis remains a persistent infection in ruminants in Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms in Punjab, Pakistan. The samples were tested by rose bengal plate agglutination test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). The seroprevalence along with 95% confidence interval (CI) was determined. Univariable and multivariable analysis of the epidemiological background data was conducted and odds ratio (OR) was calculated to understand any association between the risk factors and the seroprevalence. An overall seroprevalence of 3.9% (Positive/Tested = 32/828) and 3.3% (27/828) was detected by RBPT and iELISA, respectively. The seroprevalence of 5.6% (CI 3.6–8.3) and 4.7%, (CI 2.8–7.2) and the odds ratio of 2.63 (CI 1.20–5.77) and 2.50 (CI 1.08–5.78) for testing positive by RBPT and iELISA, respectively were significantly higher (p < 0.05) in buffaloes than in cattle. Breed, sex, history of abortion and retention of fetal membranes (RFM) in the animals were not found statistically significantly associated with the infection. RBPT and iELISA based results agreed almost perfect (k = 0.877). In total, Brucella abortus-DNA (9/27) was amplified from seropositive samples by real-time polymerase chain reaction. This study identified for the first time the etiological agents of brucellosis at a molecular level at institutional-owned livestock farms in Pakistan.

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“…[14,15]. Vaccination and treatment of brucellosis in farm animals are not considered 100% safe for human health, hence are forbidden in many countries [7,[16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Jamil

Melzer

Saqib

et al. 2020

IJERPH

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“…Isolation of the bacteria remains a gold standard but is hazardous for human health and requires advanced biosafety levels (i.e., level 3) and good training. Despite availability of newer and effective antimicrobials, the treatment of farm animals for brucellosis is generally forbidden as it poses significant public health risks [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan

et al. 2020

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Brucellosis, globally known bacterial zoonosis, is endemic to Pakistan. B. abortus in bovines, B. melitensis in small ruminants and B. canis in dogs mainly cause this disease. A total of 1821 sera (1196 from sheep and 625 from goats) from animal herds near the Pakistan–Afghanistan border were collected. In parallel testing of sera for anti-Brucella antibodies (B. abortus and B. melitensis) was carried out by RBPT and indirect ELISA. The presence of Brucella DNA in sera was tested by real-time PCR. The overall percentage of seropositive samples was 0.99 (18/1821) by both tests. All positive samples originated from Baluchistan territory which translated into 1.76% (18/1021). None of the positive sera had signals for Brucella DNA and none of sera from goats carried detectable antibodies. Both tests showed an almost perfect agreement with Kappa statistics. The flock size was found to be associated with the presence of anti-Brucella antibodies. The samples of Khyber Pakhtunkhwa (KPK) tested negative in both serological tests and hence were not processed for real-time PCR. The present study shows the presence of anti-Brucella antibodies in sheep in the Baluchistan region of Pakistan. Diagnostic services need to be improved and test and slaughter policies might be implemented for eradication of Brucella infection in these areas. Awareness about the infection is needed at the farmer’s level. Isolation and molecular biology of the isolates could help with understanding the prevailing etiology in a better way.

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“…Iannino et al designed the Bab-pgm strain (genetically engineered live B. abortus vaccine) as a heterologous carrier for the recombinant chimeric antigen to deliver Shiga toxin-producing E. coli (STEC) in a mouse model, which resulted in the induction of a protective immune response against two very different pathogens ( 162 ). Another approach to vaccine development is the use of a modified Brucella immunodominant antigen instead of deleting Brucella antigens or epitopes or introducing a foreign antigen, which could induce differential antibodies against B. ovis ( 163 ). Another approach to vaccine production is polysaccharide conjugate vaccines which are produced via the covalent glycan-protein conjugation of bacterial surface; they have been proven to be cost-effective tools to prevent dramatic infectious diseases.…”

Section: Other Vaccinesmentioning

confidence: 99%

Evaluation of Brucellosis Vaccines: A Comprehensive Review

Heidary

Dashtbin

Ghanavati³

et al. 2022

Front. Vet. Sci.

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Brucellosis is a bacterial zoonosis caused by Brucella spp. which can lead to heavy economic losses and severe human diseases. Thus, controlling brucellosis is very important. Due to humans easily gaining brucellosis from animals, animal brucellosis control programs can help the eradication of human brucellosis. There are two popular vaccines against animal brucellosis. Live attenuated Brucella abortus strain 19 (S19 vaccine) is the first effective and most extensively used vaccine for the prevention of brucellosis in cattle. Live attenuated Brucella melitensis strain Rev.1 (Rev.1 vaccine) is the most effective vaccine against caprine and ovine brucellosis. Although these two vaccines provide good immunity for animals against brucellosis, the expense of persistent serological responses is one of the main problems of both vaccines. The advantages and limitations of Brucella vaccines, especially new vaccine candidates, have been less studied. In addition, there is an urgent need for new strategies to control and eradicate this disease. Therefore, this narrative review aims to present an updated overview of the available different types of brucellosis vaccines.

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Rev1 wbdR tagged vaccines against Brucella ovis

Cited by 10 publications

References 33 publications

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan

Evaluation of Brucellosis Vaccines: A Comprehensive Review

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