Retrieval of p53 Protein in Paraffin-Embedded Head and Neck Tumor Tissues

Immunohistochemical detection of p53 in archival formalin‐fixed tissues of lip and intraoral squamous cell carcinomas from Norway

“…The study also showed that expression as well as detection of p53 in malignant neoplasms is dependent upon methods of tissue processing, fixation and antigen recovery. The level reported in this study was higher than that reported in other studies (3,5,17,27,28) which suggested low level and staining intensity of p53 in formalin-fixed tissue specimens compared to fresh frozen ones. Expression of p53 has previously also been studied in formalin-fixed tissue specimens of head and neck cancer (11,17,26,28,30,33) with reported levels ranging from 34 to 67%.…”

Prognostic Indicators for Squamous Cell Carcinoma of the Oral Cavity: A Clinicopathologic Correlation

“…A number of studies have suggested that p53 protein accumulation occurs early in the progression of head and neck squamous cell carcinoma 21‐23 . Recent reports by us and others have noted no significant association between immunohistochemical detection of p53 protein and survival among patients with advanced head and neck cancer 5,24‐26 . This study confirms that although p53 protein accumulation occurs early in the pathogenesis of oral cavity cancer, it does not appear to have prognostic significance.…”

Antigen retrieval: p53 staining in benign, pre-malignant and malignant tissues of the larynx

“…It has been reported that antigen retrieval of paraffin-embedded head and neck tumour tissues by microwave heatingI5 has been shown to increase p.53 immunostaining. 16 Thus, this technique may prove useful in detecting lower levels of p53 in human squamous carcinoma. Our study includes examination of benign laryngeal lesions as well as carcinoma in situ and squamous carcinomas.…”