RETRACTED: Metallic Ions Encapsulated in Electrospun Nanofiber for Antibacterial and Angiogenesis Function to Promote Wound Repair

Abstract

Electrospun nanofiber is an attractive biomaterial for skin tissue engineering because it mimics the natural fibrous extracellular matrix structure and creates a physical structure suitable for skin tissue regeneration. However, endowing the nanofibrous membranes with antibacterial and angiogenesis functions needs to be explored. In the current study, we aimed to fabricate gelatin/polycaprolactone (GT/PCL) (GT/PCL-Ag-Mg) nanofibers loaded with silver (Ag) and magnesium (Mg) ions for antibacterial activity and pro-angiogenesis function for wound repair. The fabricated GT/PCL membranes had a nanofibrous structure with random arrangement and achieved sustained release of Ag and Mg ions. In vitro results indicated that the GT/PCL-Ag-Mg membranes presented satisfactory cytocompatibility with cell survival and proliferation. In addition, the membranes with Ag demonstrated good antibacterial capacity to both gram-positive and gram-negative bacteria, and the Mg released from the membranes promoted the tube formation of vascular endothelial cells. Furthermore, in vivo results demonstrated that the GT/PCL-Ag-Mg membrane presented an accelerated wound healing process compared with GT/PCL membranes incorporated with either Ag or Mg ions and pure GT/PCL alone. Superior epidermis formation, vascularization, and collagen deposition were also observed in GT/PCL-Ag-Mg membrane compared with the other membranes. In conclusion, a multifunctional GT/PCL-Ag-Mg membrane was fabricated with anti-infection and pro-angiogenesis functions, serving as a potential metallic ion-based therapeutic platform for applications in wound repair.

Ascorbic acid 2-glucoside preconditioning enhances the ability of bone marrow mesenchymal stem cells in promoting wound healing

“…The skin wound images were imported into Image J software (National Institutes of Health, USA) for wound healing assay. The wound closure rate was calculated using (W 0 —W t )/W0 × 100%, where W 0 represented the initial wound area and W t represented the wound area at the corresponding measurement point [ 15 ]. This animal experiment was approved by the ethical committee of Tongji Hospital.…”

Electrospun Nanofibers Revisited: An Update on the Emerging Applications in Nanomedicine

“…Similarly, other researchers developed wound dressing based on metallic ions such as silver and magnesium [ 164 ]. The wound dressing consisted of gelatin, PCL NFs loaded with metallic ions.…”

“…could sustain release for about five days, which was the main requirement for effective antibacterial action (Figure 7). Similarly, other researchers developed wound dressing based on metallic ions such as silver and magnesium [164]. The wound dressing consisted of gelatin, PCL NFs loaded with metallic ions.…”

“…The in vivo experiments conducted on rats showed their potential wound healing characteristics. Similarly, other researchers developed wound dressing based on metallic ions such as silver and magnesium [164]. The wound dressing consisted of gelatin, PCL NFs loaded with metallic ions.…”

Nanomaterials in Skin Regeneration and Rejuvenation

“…Moreover, nanofibers produced with PCL and gelatine displayed a faster healing rate, when exopolysaccharide was loaded into a nanofiber on full-thickness wounds in rat models [112]. Furthermore, PCL nanofibers loaded with silver and magnesium ions showed antibacterial activity and pro-angiogenesis function for wound repair on vascular endothelial cells in vitro [113]. When Ag-doped magnetite nanoparticles were used, the resulting PCL scaffold had enhanced cell adhesion and growth [114].…”