RETRACTED: Development of thermosensitive hydrogel of Amphotericin-B and Lactoferrin combination-loaded PLGA-PEG-PEI nanoparticles for potential eradication of ocular fungal infections: In-vitro, ex-vivo and in-vivo studies

Elhabal, Sammar Fathy; Ghaffar, Shrouk A.; Hager, Raghda; Elzohairy, Nahla A.; Khalifa, Mohamed Mansour; Mohie, Passant M.; Gad, Rania A.; Omar, Nesreen Nabil; Elkomy, Mohammed H.; Khasawneh, Mohammad A.; Abdelaal, Nashwa

doi:10.1016/j.ijpx.2023.100174

Cited by 13 publications

(29 citation statements)

References 60 publications

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“…We evaluated the dynamic size of the particles in the solution by monitoring the electrophoretic mobility of the samples and using dynamic light scattering (DLS) at a 90-degree scattering angle and a 25-degree Celsius temperature. 18 , 19 …”

Section: Methodsmentioning

confidence: 99%

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Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

Elhabal,

Abdelaal,

Saeed Al-Zuhairy

et al. 2024

IJN

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Background Diabetes Mellitus is a multisystem chronic pandemic, wound inflammation, and healing are still major issues for diabetic patients who may suffer from ulcers, gangrene, and other wounds from uncontrolled chronic hyperglycemia. Marshmallows or Althaea officinalis (A.O.) contain bioactive compounds such as flavonoids and phenolics that support wound healing via antioxidant, anti-inflammatory, and antibacterial properties. Our study aimed to develop a combination of eco-friendly formulations of green synthesis of ZnO-NPs by Althaea officinalis extract and further incorporate them into 2% chitosan (CS) gel. Method and Results First, develop eco-friendly green Zinc Oxide Nanoparticles (ZnO-NPs) and incorporate them into a 2% chitosan (CS) gel. In-vitro study performed by UV-visible spectrum analysis showed a sharp peak at 390 nm, and Energy-dispersive X-ray (EDX) spectrometry showed a peak of zinc and oxygen. Besides, Fourier transforms infrared (FTIR) was used to qualitatively validate biosynthesized ZnO-NPs, and transmission electron microscope (TEM) showed spherical nanoparticles with mean sizes of 76 nm and Zeta potential +30mV. The antibacterial potential of A.O.-ZnO-NPs-Cs was examined by the diffusion agar method against Gram-positive ( Staphylococcus aureus and Bacillus subtilis ) and Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ). Based on the zone of inhibition and minimal inhibitory indices (MIC). In addition, an in-silico study investigated the binding affinity of A.O . major components to the expected biological targets that may aid wound healing. Althaea Officinalis , A.O-ZnO-NPs group showed reduced downregulation of IL-6, IL-1β, and TNF-α and increased IL-10 levels compared to the control group signaling pathway expression levels confirming the improved anti-inflammatory effect of the self-assembly method. In-vivo study and histopathological analysis revealed the superiority of the nanoparticles in reducing signs of inflammation and wound incision in rat models. Conclusion These biocompatible green zinc oxide nanoparticles, by using Althaea Officinalis chitosan gel ensure an excellent new therapeutic approach for quickening diabetic wound healing.

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Section: Methodsmentioning

confidence: 99%

“…Natural light was used to assess the clarity, and we made macroscopic measurements, comparing them to chitosan. 17 , 18 …”

Section: Methodsmentioning

confidence: 99%

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Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

Elhabal,

Abdelaal,

Saeed Al-Zuhairy

et al. 2024

IJN

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“…Elhabal et al combined both bovine lactoferrin and amphotericin-B loaded in triblock polymers PLGA-PEG-PEI nanoparticles by immersing the drugs-loaded nanoparticles in P188-P407 thermosensitive gel. [20] Ultimately, the innovative combination of drugs allows for higher corneal permeation and sustained release of active ingredients. Abbas et al suggested the use of thermosensitive poloxamer-N407 to prolong the effects of oxytetracycline against P. aeruginosa infection.…”

Section: Keratitismentioning

confidence: 99%

Smart molecules in ophthalmology: Hydrogels as responsive systems for ophthalmic applications

Kulbay,

Wu,

Truong

et al. 2024

Smart Molecules

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This comprehensive review delves into a unique intersection of hydrogels as smart molecules and their transformative applications in ophthalmology. Beginning with the foundational definition, properties, and classification of hydrogels, the review explores their synthesis and responsive capabilities. Specific applications examined encompass topical drug delivery, contact lenses, intravitreal drug delivery, ocular adhesives, vitreous substitutes, and cell‐based therapy. A methodical analysis, including an overview of relevant ocular structures and a comparative evaluation of hydrogel‐based solutions against traditional treatments, is conducted. Additionally, potential constraints, translation challenges, knowledge gaps, and research areas are identified. Our methodical approach, guided by an extensive literature review from 2017 to 2023, illuminates the unprecedented opportunities offered by hydrogels, along with pinpointing areas for further inquiry to facilitate their transition into clinical practice.

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“…Based on this study, the antifungal feature of A‐B1‐nanoparticles formulation was statistically higher than A1‐nanoparticles formulation against all investigated strains, where greater inhibition zones were generated. [ 55 ] Hence, newly designed formulation of Am‐B can be more effective against fungal infections.…”

Section: Clinical Efficacy Of Am‐b Against Fungal Infectionsmentioning

confidence: 99%

Developing nanosize carrier systems for Amphotericin‐B: A review on the biomedical application of nanoparticles for the treatment of leishmaniasis and fungal infections

Sharifi,

Alitaneh,

Asadi

et al. 2023

Biotechnology Journal

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New formulations of Amphotericin‐B (Am‐B), the most popular therapeutic drug for many human infections such as parasitic and fungal pathogens, are safe, economical, and effective in the world. Several newly designed carrier systems for Am‐B can also be considered orally with sufficient gastrointestinal permeability and good solubility. However, the clinical application of several new formulations of Am‐B with organ cytotoxicity, low bioavailability, high costs, and technical problems have caused some issues. Therefore, more attention and scientific design are required to progress safe and effective drug delivery systems. Currently, the application of nano‐based technology and nanomaterials in the advancement of drug delivery systems exhibits promising outcomes to cure many human systemic infections. Designing novel drug delivery systems including solid lipid nanostructured materials, lipo‐polymersomes, drug conjugates and microneedles, liposomes, polymer and protein‐based nanostructured materials, dendrimers, emulsions, mixed micelles, polymeric micelles, cyclodextrins, nanocapsules, and nanocochleate for Am‐B has many advantages to reducing several related issues. The unique properties of nanostructured particles such as proper morphology, small size, surface coatings, and, electrical charge, permit scientists to design new nanocomposite materials against microorganisms for application in various human diseases. These features have made these nanoparticles an ideal candidate for drug delivery systems in clinical approaches to cure a number of human disorders and currently, several therapeutic nanostructured material formulations are under different stages of clinical tests. Hence, this scientific paper mainly discussed the advances in new formulations of Am‐B for the treatment of human systemic infections and related clinical tests.

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RETRACTED: Development of thermosensitive hydrogel of Amphotericin-B and Lactoferrin combination-loaded PLGA-PEG-PEI nanoparticles for potential eradication of ocular fungal infections: In-vitro, ex-vivo and in-vivo studies

Cited by 13 publications

References 60 publications

Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

Green Synthesis of Zinc Oxide Nanoparticles from Althaea officinalis Flower Extract Coated with Chitosan for Potential Healing Effects on Diabetic Wounds by Inhibiting TNF-α and IL-6/IL-1β Signaling Pathways

Smart molecules in ophthalmology: Hydrogels as responsive systems for ophthalmic applications

Developing nanosize carrier systems for Amphotericin‐B: A review on the biomedical application of nanoparticles for the treatment of leishmaniasis and fungal infections

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