RETRACTED: Biomarkers for oxidative stress status of DNA, lipids, and proteins in vitro and in vivo cancer research

Hwang, Eun‐Sun; Kim, Gun-Hee

doi:10.1016/j.tox.2006.10.013

Cited by 130 publications

(92 citation statements)

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“…8-OHdG formation is used as an indicator of oxidative DNA damage. Measurement of 8-OHdG is frequently used to estimate oxygen radical-induced damage (26,33,35). 8-OHdG concentrations were determined in the aortic homogenates using the method originally described by Toyokuni et al (48) and modified by Garçon et al (21).…”

Section: Study Of Oxidative Stress Markersmentioning

confidence: 99%

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Bulckaen¹,

Prévost²,

Boulanger³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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The age-related impairment of endothelium-dependent vasodilatation contributes to increased cardiovascular risk in the elderly. For primary and secondary prevention, aspirin can reduce the incidence of cardiovascular events in this patient population. The present work evaluated the effect of low-dose aspirin on age-related endothelial dysfunction in C57B/J6 aging mice and investigated its protective antioxidative effect. Agerelated endothelial dysfunction was assessed by the response to acetylcholine of phenylephrine-induced precontracted aortic segments isolated from 12-, 36-, 60-, and 84-wk-old mice. The effect of low-dose aspirin was examined in mice presenting a decrease in endothelial-dependent relaxation (EDR). The effects of age and aspirin treatment on structural changes were determined in mouse aortic sections. The effect of aspirin on the oxidative stress markers malondialdehyde and 8-hydroxy-2Ј-deoxyguanosine (8-OhdG) was also quantified. Compared with that of 12-wk-old mice, the EDR was significantly reduced in 60-and 84-wk-old mice (P Ͻ 0.05); 68-wkold mice treated with aspirin displayed a higher EDR compared with control mice of the same age (83.9 Ϯ 4 vs. 66.3 Ϯ 5%; P Ͻ 0.05). Aspirin treatment decreased 8-OHdG levels (P Ͻ 0.05), but no significant effect on intima/media thickness ratio was observed. The protective effect of aspirin was not observed when treatment was initiated in older mice (96 wk of age). It was found that low-dose aspirin is able to prevent age-related endothelial dysfunction in aging mice. However, the absence of this effect in the older age groups demonstrates that treatment should be initiated early on. The underlying mechanism may involve the protective effect of aspirin against oxidative stress. endothelium; intima/media; vasodilatation; oxidative stress AGE-ASSOCIATED CHANGES SUCH as endothelial dysfunction are involved in the significantly increased risk of cardiovascular complications and microthrombus formation in the elderly patient population (44). The presence of endothelial dysfunction in the coronary or peripheral circulation has been shown to constitute a risk factor for cardiovascular events independent of the development of atherosclerosis or other vascular risk factors (22,(43)(44)(45). There is emerging evidence that age-associated endothelial dysfunction is related to the local formation of reactive oxygen and nitrogen species within and in the vicinity of the vascular wall (13,25,36,43,49). Therapeutic approaches capable of preventing or reversing age-related endothelial dysfunction may thus help to reduce cardiovascular risk in the elderly.Age-related endothelial-dependent relaxation (EDR) decreases in the large vessels of different animal species including humans (36). EDR in response to acetylcholine (ACh) decreases with age in the rat aorta: the maximal relaxation effect of ACh is 100% in 4-to 6-wk-old, 50% in 3-to 6-mo-old, and 25% in 12-to 25-mo-old rats (29). The agerelated decrease in EDR varies from one vessel to another and from one species to anot...

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Section: Study Of Oxidative Stress Markersmentioning

confidence: 99%

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Bulckaen¹,

Prévost²,

Boulanger³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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“…MDA is an end product of lipid peroxidation, and is considered a late biomarker of oxidative stress and cellular damage, and LPO is one of the main manifestations of oxidative damage and it has been found to play an important role in the toxicity and carcinogenicity [43]. Oxidative stress arises when the generation of ROS, by-products of the oxidative metabolism primarily produced in the mitochondria, exceeds the cellular ability to eliminate them and to repair cellular damage, thus leading to oxidation of biomolecules including DNA, lipids and proteins [44]. These results confirm and extend previous data which have demonstrated that AFs induce a significant increase in MDA under in vitro and in vivo conditions [15,45,46].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Lactobacillus rhamnosus GG on Aflatoxins-Induced Toxicities in Male Albino Mice

Deabes¹,

Darwish²,

B³

et al. 2012

J Environment Analytic Toxicol

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“…Similarly, short-chain hydrocarbons (e.g. pentane and ethane) generated during lipid peroxidation have been reported in the breath of humans suggestive of CCl 4 -induced oxidative stress (Hwang & Kim, 2007). These reactive aldehydes arising from lipid peroxidation have been shown to be quenched by reduced glutathione (GSH), causing GSH depletion which leads to cirrhosis in the liver of rats (Cabre et al, 2000).…”

Section: Hepatotoxicity (Carbon Tetrachloride)mentioning

confidence: 96%

Metabolomics Approach for Hazard Identification in Human Health Assessment of Environmental Chemicals

Vulimiri¹,

Pachkowski²,

Bale³

et al. 2012

Metabolomics

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RETRACTED: Biomarkers for oxidative stress status of DNA, lipids, and proteins in vitro and in vivo cancer research

Cited by 130 publications

References 0 publications

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Low-dose aspirin prevents age-related endothelial dysfunction in a mouse model of physiological aging

Protective Effects of Lactobacillus rhamnosus GG on Aflatoxins-Induced Toxicities in Male Albino Mice

Metabolomics Approach for Hazard Identification in Human Health Assessment of Environmental Chemicals

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