Retinol Binding Protein in Rainbow Trout: Molecular Properties and mRNA Expression in Tissues

Sammar, Marei; Babin, Patrick J.; Durliat, M; Meiri, Iris; Zchori, Itai; Elizur, Abigail; Lubzens, Esther

doi:10.1006/gcen.2001.7636

Cited by 31 publications

(12 citation statements)

References 46 publications

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“…We have previously shown that treatment of fish with estradiol or its analogues results in elevated concentrations of the vitamin in plasma and depleted stores in storage organs, perhaps as a result of translocation of the vitamin to the developing ovaries (Palace et al, 2001a,b). Similar results have been reported by Sammar et al (2001as cited in Lubzens et al, 2003. It appears that as spawning approaches and estrogen levels peak, there is no significant regulation of mRNA for retinol binding proteins in the liver, but there is certainly an increase of mRNA for VTG.…”

Section: Vitamin a And Early Developmentsupporting

confidence: 92%

Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review

Palace¹,

Werner²

2006

Sci. Mar.

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SUMMARY: Early development in fish is dependent on essential nutrients present in the egg. Vitamins E and A are critical to the normal development of fish embryos. Vitamin E functions primarily as an antioxidant to protect unsaturated lipids from oxidative degradation. Vitamin A or its provitamin carotenoid forms support growth, are required for the differentiation and maintenance of epithelial tissue and can be converted to forms of the vitamin that are potent morphogens. Fish cannot synthesise either of the vitamins, so the maternal dietary content of each prior to oogenesis is an important determinant of reproductive fitness. Here we review the basic metabolism of vitamins E and A and carotenoids and their processing from the maternal diet and deposition into eggs. Evidence for the link between levels of each of the vitamins in eggs and measures of reproductive fitness are also discussed. -El desarrollo embrionario en peces es dependiente de los nutrientes esenciales presentes en el huevo. Las vitaminas E y A son críticas en el desarrollo normal de los embriones de los peces. La vitamina E funciona sobre todo como un antioxidante para proteger los lípidos no saturados contra la degradación oxidativa. La vitamina A, o los carotenoides formas de provitamina, se requieren para la diferenciación y el mantenimiento del tejido fino epitelial y se puede convertir a las formas de la vitamina que son morfogenes potentes. Los peces tampoco pueden sintetizar las vitaminas, de modo que el contenido dietético materno de cada una antes de la oogenesis es un determinante importante de la aptitud reproductiva. Aquí repasamos el metabolismo básico de las vitaminas E y A y los carotenoides y su procesamiento desde la dieta materna a la deposición en los huevos. Se discute también la evidencia del acoplamiento entre los niveles de cada una de las vitaminas en los huevos y las medidas de aptitud reproductiva en los adultos.

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Section: Vitamin a And Early Developmentsupporting

confidence: 92%

Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review

Palace¹,

Werner²

2006

Sci. Mar.

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“…1). Reports concerning other teleosts indicate only weak, if any, expression of RBP in ovary [14,15]. In fact, we observed RBP cDNA expression in immature (data not shown), normal (Fig.…”

Section: Expression Of Rbp In Salmon Ovary and Testiscontrasting

confidence: 52%

“…1). The expression of RBP also was followed, because the presence of this gene had not previously been unequivocally demonstrated in either ovary or testis of fish [14,15]. The PCR products of the following sizes were generated using specific primer sets for each gene: Cg␣, 462 bp; LH␤, 587 bp; FSH␤, 414 bp; TSH␤, 549 bp; RBP, 417 bp; and ubiquitin, 158 bp.…”

Section: Identification and Confirmation Of Uniquely Expressed Genesmentioning

confidence: 99%

A Comprehensive Survey of the Genes Involved in Maturation and Development of the Rainbow Trout Ovary1

Schalburg

Rise

Brown

et al. 2005

Biology of Reproduction

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Development and maturation of the ovary requires precisely coordinated expression of specific gene classes to produce viable oocytes. We undertook identification of some of the genes involved in these processes by creating ovary-specific cDNA libraries by suppression subtractive hybridization and by microarray-based analyses. We present 5778 tissue- and sex-specific genes from subtracted ovary and testis libraries, many of which remain unidentified. A microarray containing 3557 salmonid cDNAs was used to compare the transcriptomes of precocious ovary at three different stages during the second year of life with a reference (normal ovary) transcriptome. On average, approximately 240 genes were developmentally regulated during the study period from June to October. Classes of genes maintaining relatively steady-state levels of expression, such as those controlling tissue remodeling, immunoregulation, cell-cycle progression, apoptosis, and growth also were identified. Concurrent expression of various cell division and ubiquitin-mediated proteolysis regulators revealed the utility of microarray analysis to monitor important maturation events. We also report unequivocal evidence for expression of the transcripts that encode the common glycoprotein alpha, LH beta, FSH beta, thyroid-stimulating hormone beta, and retinol-binding protein in both the ovary and testis of trout.

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“…A 16-amino-acid signal peptide was predicted, resulting in a mature protein of 176 amino acids with an estimated molecular mass of 20,223 Da. Comparison with human, chicken, and Xenopus RBP4 sequences revealed 62, 64, and 58% identity, respectively, whereas from 73 to 89% identity was found with RBP4 sequences from rainbow trout (Oncorhynchus mykiss; Sammar et al, 2001), gilthead seabream (Sparus auratus; Funkenstein, 2001), and carp (Cyprinus carpio; Bellovino et al, 2002). RBP4 belongs to the lipocalin family and is a specific, major carrier for the extracellular transport of retinol and retinal (Flower, 1996).…”

Section: Characterization Of Zebrafish Rbp4mentioning

confidence: 96%

“…In rainbow trout (Oncorhynchus mykiss), homologous transcripts have been shown to be expressed predominantly in the liver and in other adult tissues, such as the spleen, ovaries, brain, and kidneys (Sammar et al, 2001;Lubzens et al, 2003), whereas seabream (Sparus aurata) transcripts have also been detected in adult skin using reverse transcriptase-polymerase chain reaction (RT-PCR;Funkenstein, 2001). Breakdown by tissue of human and or mouse UniGene (http:// www.ncbi.nlm.nih.gov/entrez/query.…”

Section: Epidermis Molecular Patterning and The Role Of Rbp4 Apoeb mentioning

confidence: 99%

Epidermal transient down‐regulation of retinol‐binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

Tingaud‐Sequeira¹,

Forgue²,

André³

et al. 2006

Developmental Dynamics

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Very little is known about the molecular control of skin patterning and scale morphogenesis in teleost fish. We have found radially symmetrical epidermal placodes with down-regulation of retinol-binding protein 4 (rbp4) expression during the initial paired fin and scale morphogenesis in zebrafish (Danio rerio). This finding may be related to changes in keratinocyte cytodifferentiation and/or the integument retinoid metabolism. rbp4 transcripts are expressed afterward in the central epidermis of the scale papilla and gradually extend to the epidermis, covering the growing scale, whereas no transcripts were detected in posterior margin epidermis. In contrast, induction of apolipoprotein Eb (apoeb) and up-regulation of estrogen receptor 2a (esr2a) transcripts were observed in the epidermis at initiator sites of zebrafish ectodermal/dermal appendage morphogenesis. This expression was maintained in the posterior margin epidermis of the formed scales. esr2a was also strongly expressed in neuromasts, whereas no rbp4 and apoeb transcripts were detected in these mechanosensory structures. The observed epidermal molecular events suggest that epidermis patterning is due to an activator-inhibitor mechanism operational at epidermal-dermal interaction sites. rbp4 transcript expression was also strongly down-regulated by 1-phenyl-2-thio-urea (PTU). As this inhibitor is commonly used to block obscuring pigmentation during in situ hybridization studies, this finding suggests that PTU should be used with caution, particularly in studying skin development. Developmental Dynamics 235:3071-3079, 2006.

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Retinol Binding Protein in Rainbow Trout: Molecular Properties and mRNA Expression in Tissues

Cited by 31 publications

References 46 publications

Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review

Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review

A Comprehensive Survey of the Genes Involved in Maturation and Development of the Rainbow Trout Ovary1

Epidermal transient down‐regulation of retinol‐binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

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