Retinal detachment: visual acuity and subretinal immunoreactive endothelin-1

Modelling postoperative visual acuity with and without proliferative vitreoretinopathy associated with primary rhegmatogenous retinal detachment

“…SRF was obtained during the drainage procedure of the RD surgery, 23 as previously reported. 18,24 Briefly, after a 1-mm sclerotomy and local diathermic coagulation of the choroids at the site of maximum prominence of the detached retina, a plastic disposable fine-tip transfer pipet (Life Scan Inc., Milpitas, CA, USA) was used to obtain the SRF. Both SRF and blood samples were collected in chilled tubes containing protease inhibitors.…”

“…IR-ET-1 measurements were taken from plasma and SRF samples as previously reported. 6,17,18,24 Briefly, IR-ET-1 was assayed in the laboratory by RIA, using polyclonal antibodies against synthetic ET-1 (Peninsula Laboratories, Merseyside, UK) at a 1 : 90 000 final dilution. The sensitivity of the RIA was 1 pg/ml.…”

Preoperative duration of retinal detachment and preoperative central retinal artery hemodynamics: repercussion on visual acuity

“…Eyes with longer RD duration, proliferative vitreoretinopathy (PVR) of any type, and severe PVR have worse initial and final visual acuities than eyes with shorter RD duration or those without PVR respectively [3][4][5].…”

Preoperative duration of retinal detachment and subretinal immunoreactive endothelin-1: repercussion on logarithmic visual acuity

“…duration, proliferative vitreoretinopathy (PVR) of any type, and severe PVR have worse initial and final visual acuities than eyes with shorter RD duration or those without PVR respectively [5][6][7]. Preoperative, postoperative 8 months LogMAR (logarithm of the minimum angle of resolution) VAs, and preoperative ocular blood flow [central retinal artery (CRA) parameters] were influenced by preoperative RD duration in both no PVR and PVR [8], and preoperative CRA parameters were in turn correlated with LogMAR VAs (preoperative, postoperative 8 months and difference: postoperative 8 months minus preoperative) in PVR [8].…”

“…Previously we have reported the relationship between increased immunoreactive ET-1 (IR-ET-1) levels in the eye [in the vitreous and in subretinal fluid (SRF)] and the proliferative complications of retinal detachment [13,14]. The higher the IR-ET-1 levels the lower the postoperative VA, and the lower the VA difference (postoperative 8 months minus preoperative) in RD [7]. The highest negative correlations between VA (postoperative and difference:postoperative 8 months minus preoperative) and the SRF IR-ET-1 levels were found in the PVR group [7].…”