Retention mechanisms for ER and Golgi membrane proteins

Gao, Caiji; Cai, Yi; Wang, Yejun; Kang, Byung-Ho; Aniento, Fernando; Robinson, David G.; Jiang, Liwen

doi:10.1016/j.tplants.2014.04.004

Cited by 90 publications

(75 citation statements)

References 75 publications

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“…The comparatively larger GFP moiety (*240 aa) may disrupt the transport of the fused RCI2 by masking unknown targeting signals or by steric hindrance. It is known that in the absence of targeting, membrane proteins in the secretory pathway are delivered by default to the plasma membrane or to vacuoles (Gao et al 2014).…”

Section: Rci2 Proteins: Structure Localization and Architecture In Mmentioning

confidence: 99%

Plant abiotic stress-related RCI2/PMP3s: multigenes for multiple roles

Rocha

2015

Planta

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RCI2 / PMP3 s participate in abiotic stress responses and impact the expression of other genes. Their multifunctionality is determined by differential expression and by distinct activities of their structurally different proteins. In plants, RCI2/PMP3 genes, which encode small membrane proteins of the PMP3 family, are closely associated with abiotic stress responses. Their involvement in mediating stress tolerance is supported by genetic evidence and overexpression studies. RCI2/PMP3s occur as multigenes in plant genomes and their encoded proteins belong to distinct and conserved structural groups. In addition, different isoforms appear to be targeted to the plasma membrane or to distinct endomembrane compartments in cells. Several studies have revealed that RCI2/PMP3 proteins participate in cell ion homeostasis, and in regulation of membrane stability and polarization. They also appear to potentiate plant transcriptional responses to abiotic stresses. However, their mechanisms of action remain unknown. This paper reviews the current knowledge of the multiple roles of plant RCI2/PMP3 genes resulting from their differential expression under normal and stress conditions. The structural diversity of RCI2/PMP3 proteins is analyzed and evidence supporting their functional specialization and possible activity mechanisms is examined. Finally, strategies are discussed for exploiting new and established technologies to overcome the difficulties posed by the multigene status of RCI2s and the integral membrane character of their proteins, enabling the probing of their individual functions and collective significance.

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Section: Rci2 Proteins: Structure Localization and Architecture In Mmentioning

confidence: 99%

Plant abiotic stress-related RCI2/PMP3s: multigenes for multiple roles

Rocha

2015

Planta

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“…Endomembrane trafficking in plant cells is complicated such that secretory, endocytic, and recycling pathways are usually integrated with each other at the post-Golgi compartments, among which, the trans-Golgi network (TGN) and prevacuolar compartment (PVC)/multivesicular body (MVB) are best studied (Tse et al, 2004;Lam et al, 2007aLam et al, , 2007bMüller et al, 2007;Foresti and Denecke, 2008;Hwang, 2008;Otegui and Spitzer, 2008;Robinson et al, 2008;Richter et al, 2009;Ding et al, 2012;Gao et al, 2014). Following the endocytic trafficking of a lipophilic dye, FM4-64, the TGN and PVC/MVB are sequentially labeled and thus are defined as the early and late endosome, respectively, in plant cells (Lam et al, 2007a;Chow et al, 2008).…”

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confidence: 99%

The Arabidopsis Endosomal Sorting Complex Required for Transport III Regulates Internal Vesicle Formation of the Prevacuolar Compartment and Is Required for Plant Development

et al. 2014

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We have established an efficient transient expression system with several vacuolar reporters to study the roles of endosomal sorting complex required for transport (ESCRT)-III subunits in regulating the formation of intraluminal vesicles of prevacuolar compartments (PVCs)/multivesicular bodies (MVBs) in plant cells. By measuring the distributions of reporters on/within the membrane of PVC/ MVB or tonoplast, we have identified dominant negative mutants of ESCRT-III subunits that affect membrane protein degradation from both secretory and endocytic pathways. In addition, induced expression of these mutants resulted in reduction in luminal vesicles of PVC/MVB, along with increased detection of membrane-attaching vesicles inside the PVC/MVB. Transgenic Arabidopsis (Arabidopsis thaliana) plants with induced expression of ESCRT-III dominant negative mutants also displayed severe cotyledon developmental defects with reduced cell size, loss of the central vacuole, and abnormal chloroplast development in mesophyll cells, pointing out an essential role of the ESCRT-III complex in postembryonic development in plants. Finally, membrane dissociation of ESCRT-III components is important for their biological functions and is regulated by direct interaction among Vacuolar Protein SortingAssociated Protein20-1 (VPS20.1), Sucrose Nonfermenting7-1, VPS2.1, and the adenosine triphosphatase VPS4/SUPPRESSOR OF K 1 TRANSPORT GROWTH DEFECT1.Endomembrane trafficking in plant cells is complicated such that secretory, endocytic, and recycling pathways are usually integrated with each other at the post-Golgi compartments, among which, the trans-Golgi network (TGN) and prevacuolar compartment (PVC)/multivesicular body (MVB) are best studied (Tse et al

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“…These drugs were diluted in germination medium to appropriate working concentrations before incubation with germinating pollen tubes. For each drug treatment, germinating pollen tubes or BY2 cells were mixed with the drugs in working solutions in the medium at a 1:1 ratio to minimize sample variation, and such a method of drug treatment has been well-documented with success in both pollen tubes and BY2 cells of previous studies (Tse et al, 2004;Tse et al, 2006;Robinson et al, 2008;Lam et al, 2009;Wang et al, 2010a;Cai et al, 2011;Wang et al, 2011b;Zhuang et al, 2013;Cui et al, 2014;Ding et al, 2014;Gao et al, 2014b;Gao et al, 2015;Shen et al, 2016;Wang et al, 2016;Chung et al, 2016). The JIM5 and JIM7 antibodies were purchased from the Paul Knox Cell Wall Lab at the University of Leeds (Plant 710Probes).…”

Section: Drug Treatment and Antibodiesmentioning

confidence: 99%

A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation

et al. 2016

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Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway that mediates cell wall formation during cell expansion and cytokinesis. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that Golgi-derived secretory vesicles (GDSVs) labeled by NtPPME1-GFP are distinct from those organelles belonging to the conventional post-Golgi exocytosis pathway. In addition, pharmaceutical treatments, superresolution imaging, and dynamic studies suggest that NtPPME1 follows a polar exocytic process from Golgi-GDSV-PM/cell plate (CP), which is distinct from the conventional Golgi-TGN-PM/CP secretion pathway. Further studies show that ROP1 regulates this specific polar exocytic pathway. Taken together, we have demonstrated an alternative TGN-independent Golgi-to-PM polar exocytic route, which mediates secretion of NtPPME1 for cell wall formation during cell expansion and cytokinesis and is ROP1-dependent.

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Retention mechanisms for ER and Golgi membrane proteins

Cited by 90 publications

References 75 publications

Plant abiotic stress-related RCI2/PMP3s: multigenes for multiple roles

Plant abiotic stress-related RCI2/PMP3s: multigenes for multiple roles

The Arabidopsis Endosomal Sorting Complex Required for Transport III Regulates Internal Vesicle Formation of the Prevacuolar Compartment and Is Required for Plant Development

A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation

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