Resveratrol suppresses pulmonary tumor metastasis by inhibiting platelet-mediated angiogenic responses

He, Lin; Fan, Fangtian; Hou, Xianbang; Gao, Cuixiang; Li, Meng; Shu, Meng; Huang, Shi‐Wen; Wu, Hongyan

doi:10.1016/j.jss.2017.05.009

Cited by 17 publications

(14 citation statements)

References 51 publications

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“…cGMP is an important intracellular signal transduction molecule with both pro‐ and anticancer effects (Dhayade et al ., ; Tinsley et al ., ). For instance, cGMP has been shown to inhibit ADP‐induced platelet‐mediated angiogenic responses in the adenocarcinomic human alveolar epithelial cell line (A549) (He et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer

et al. 2019

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Lung cancer is the leading cause of cancer-related deaths in the world. The most prevalent subtype, accounting for 85% of cases, is non-small-cell lung cancer (NSCLC). Lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) are the most common subtypes. Despite recent advances in treatment, the low 5-year survival rate of NSCLC patients (approximately 13%) reflects the lack of early diagnostic biomarkers and incomplete understanding of the underlying disease mechanisms. We hypothesized that integration of metabolomic, transcriptomic and genetic profiles of tumours and matched normal tissues could help to identify important factors and potential therapeutic targets that contribute to tumorigenesis. We integrated omics profiles in tumours and matched adjacent normal tissues of patients with LUSC (N = 20) and LUAD (N = 17) using multiple system biology approaches. We confirmed the presence of previously described metabolic pathways in NSCLC, particularly those mediating the Warburg effect. In addition, through our combined omics analyses we found that metabolites and genes that contribute to haemostasis, angiogenesis, platelet activation and cell proliferation were predominant in both subtypes of NSCLC. The important roles of adenosine diphosphate in promoting cancer metastasis through platelet activation and angiogenesis suggest this metabolite could be a potential therapeutic target.Abbreviations ADP, adenosine diphosphate; HMDB, Human Metabolome Database; LC/MS, liquid chromatography/mass spectrometry; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small-cell lung cancer; WGCNA, weighted gene co-expression network analysis.

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Section: Discussionmentioning

confidence: 97%

Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer

et al. 2019

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“…As is known, RST is reported to exert anti‐oxidant, anti‐inflammatory, and anti‐tumour activities . Moreover, numerous investigators have described a regulatory effect of RST on pathologic angiogenesis, including our previous study . A major challenge to date that remains, however, is our limited understanding regarding the detailed molecular mechanisms by which RST may influence angiogenesis.…”

Section: Introductionmentioning

confidence: 88%

“…Capillary tube formation was performed as we described previously . Briefly, Matrigel was added into 24‐well plates (100 μL/well) and solidified in a 37°C incubator.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, Matrigel was added into 24‐well plates (100 μL/well) and solidified in a 37°C incubator. Afterwards, primary HUVECs in serum‐free medium were inoculated into 24‐well plates (1 × 10 5 /well) and pretreated by RST for 24 hours, then HUVECs were washed extensively to remove the remaining compound as we previously described, and 20 ng/mL VEGF was added except the control group. Capillary tube formation was quantified after 18 hours incubation.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemical assay was conducted according to our previous study. Briefly, sponge tissue sections were incubated with primary antibodies against CD34 for immunohistochemical evaluation using standard methods . Microvascular density (MVD) was measured under a light microscope by selecting a single area of section (200) representative with the highest one (neovascular hot spot).…”

Section: Methodsmentioning

confidence: 99%

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Resveratrol inhibits VEGF‐induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocation

Shi³

et al. 2018

Clin Exp Pharma Physio

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Endothelial cells (ECs) mainly depend on aerobic glycolysis to generate angiogenesis. Deregulation of glycolysis is often observed in human endothelial cells during angiogenesis. In the present study, we first report that resveratrol (RST), which has been intensively studied in glucose metabolism of various cancer cells, has a profound inhibitory effect on tube formation and migration via suppression of glycolysis in human umbilical vein endothelial cells (HUVECs) induced by vascular endothelial growth factor (VEGF). Moreover, we further reveal that RST reduced the mRNA and protein level of glucose transporter-1(GLUT1), hexokinase II (HK2), phosphofructokinase-1(PFK1) and pyruvate kinase M2 (PKM2) through modulation of ERK-mediated PKM2 nuclear translocation. Our results provide a novel mechanism to account for the inhibition of RST on VEGF-mediated angiogenesis and suggest that targeting aerobic glycolysis or nuclear PKM2 may be a new approach for pathological angiogenesis prevention or treatment.

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Immune Cells: Critical Players in Drug Resistance

Khan,

Jan,

Fatima

et al. 2024

Drug Resistance in Cancer: Mechanisms and Strategies

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Resveratrol suppresses pulmonary tumor metastasis by inhibiting platelet-mediated angiogenic responses

Cited by 17 publications

References 51 publications

Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer

Metabolomic, transcriptomic and genetic integrative analysis reveals important roles of adenosine diphosphate in haemostasis and platelet activation in non‐small‐cell lung cancer

Resveratrol inhibits VEGF‐induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocation

Immune Cells: Critical Players in Drug Resistance

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