Resveratrol Partially Prevents Rotenone-Induced Neurotoxicity in Dopaminergic SH-SY5Y Cells through Induction of  Heme Oxygenase-1 Dependent Autophagy

Lin, Tsu-Kung; Chen, Shang-Der; Chuang, Yao-Chung; Lin, Hung‐Yu; Huang, Chi-Ren; Chuang, Jiin‐Haur; Wang, Pei‐Wen; Huang, Sheng‐Teng; Tiao, Mao‐Meng; Chen, Jin‐Bor; Liou, Chia‐Wei

doi:10.3390/ijms15011625

Cited by 144 publications

(88 citation statements)

References 66 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SES (100 µM) alone treated cells show no alterations in nuclear morphology and are similar to vehicle control. Lin et al reported that resveratrol, a polyphenolic stilbene prevented nuclear damage induced by ROT [42] and this correlates with our study.…”

Section: Resultssupporting

confidence: 93%

Sesamol Antagonizes Rotenone-Induced Cell Death in Sh-Sy5y Neuronal Cells

Rohini

Krishnamurthy

2016

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: To investigate the neuroprotective effect of sesamol against rotenone-induced cell death in SH-SY5Y cells associated with Parkinsonism.Methods: SH-SY5Y cells were maintained in Dulbecco's modified Eagle's medium. After differentiation, the cells were incubated with rotenone (20 μM) and sesamol at different concentrations (10-100 μM). Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. The reactive oxygen species, mitochondrial membrane potential and nuclear morphology were determined by dichlorofluorescein diacetate, rhodamine 123 and 4', 6-diamidino-2-phenylindole, respectively. Thiobarbituric acid reactive substances, activities of catalase, superoxide dismutase, and glutathione peroxidase and glutathione level were determined by standard assays. Results:Sesamol significantly increased the cell viability and decreased the rotenone-induced cell death in SH-SY5Y cells. Sesamol antagonized rotenone-induced reactive oxygen species generation, loss of mitochondrial membrane potential and nuclear damage. Sesamol also decreased thiobarbituric acid reactive substances level, increased the activities of catalase, superoxide dismutase, glutathione peroxidase and increased the level of glutathione in rotenone-induced cells. Conclusion:The results obtained strongly indicate the promising neuroprotective role of sesamol against rotenone-induced death in SH-SY5Y cells.

show abstract

Section: Resultssupporting

confidence: 93%

Sesamol Antagonizes Rotenone-Induced Cell Death in Sh-Sy5y Neuronal Cells

Rohini

Krishnamurthy

2016

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

show abstract

“…Resveratrol (RSV) is a naturally occurring polyphenolic phytoalexin that is produced by polygonum cuspidatum, mulberry (Morus species), grapes, and red wine. It has diverse biological activities including anti-oxidation [21] , anti-cancer [22] , anti-inflammation [23] , cardioprotection [24] , and neuroprotection [25] . Several reports indicate that RSV is capable of preventing and treating diabetes and its related complications [26,27] .…”

Section: Introductionmentioning

confidence: 99%

Resveratrol ameliorates experimental periodontitis in diabetic mice through negative regulation of TLR4 signaling

et al. 2014

View full text Add to dashboard Cite

Aim: To investigate the therapeutic effects of resveratrol (RSV) on periodontitis in diabetic mice and to explore the underlying mechanisms in vitro. Methods: Experimental periodontitis was induced in db/db mice by ligature application of porphyromonas gingivalis. The mice were treated with RSV (20 mg/kg, po) daily for 4 weeks. Alveolar bone loss, proinflammatory cytokines and TLR4 expression in the gingival tissue were measured. Cultured gingival epithelial cells (GECs) were used for in vitro studies. The transcriptional activity of TLR4 downstream signaling was analyzed using Western blotting. Results: RSV administration significantly decreased the blood glucose levels, and ameliorated alveolar bone loss in db/db mice with experimental periodontitis. RSV administration also suppressed the high levels of IL-1β, IL-6, IL-8, TNF-α, and TLR4 in gingival tissue of the mice. In the GECs incubated in high glucose medium, TLR4 expression was substantially upregulated, which was partly blocked in the presence of RSV. Lipopolysaccharides markedly increased the expression and secretion of IL-1β, IL-6, IL-8, and TNF-α in the GECs cultured in high glucose medium, which was also partly blocked in the presence of RSV. Furthermore, RSV significantly suppressed the phosphorylation of TLR4 downstream factors NF-κB p65, p38MAPK, and STAT3. Conclusion: RSV exerts protective effects against experimental periodontitis in db/db mice via negative regulation of TLR4 signaling.

show abstract

“…For example, carnosic acid protects against 6-hydroxydopamine (6-OHDA)-induced apoptosis by inducing autophagy in SH-SY5Y cells [35]. Similarly, resveratrol protects against rotenone-induced neuronal apoptosis by regulating autophagic flux [36]. …”

Section: Introductionmentioning

confidence: 99%

Isoflavones Induce BEX2-Dependent Autophagy to Prevent ATR-Induced Neurotoxicity in SH-SY5Y Cells

Ma¹,

Wu²,

Wu³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Atrazine (ATR) is a broad-spectrum herbicide in wide use around the world. However, ATR is neurotoxic and can cause cell death in dopaminergic neurons, leading to neurodegenerative disorders. Autophagy is the basic cellular catabolic process involving the degradation of proteins and damaged organelles. Studies have shown that certain plant compounds can induce autophagy and prevent neuronal cell death. This prompted us to investigate plant compounds that might reduce the neurotoxic effects of ATR. Methods: By CCK-8 and flow cytometry, we tested the ability of five candidate compounds—isoflavones, resveratrol, quercetin, curcumin, and green tea polyphenols—to protect cells from ATR. Changes in the expression of tyrosine hydroxylase (TH) and brain-expressed X-linked 2 (BEX2), autophagy-related proteins and key factors in mTOR signaling, were detected by Western blotting. Results: Isoflavones had the strongest activity against ATR-induced neuronal apoptosis. ATR reduced the expression of TH and BEX2, whereas isoflavones increased TH and BEX2 expression. In addition, ATR inhibited autophagy, whereas isoflavones induced autophagy through the accumulation of LC3-II and decreased expression of p62; this effect was abolished by 3-methyladenine (3-MA). Furthermore, BEX2 siRNA abolished isoflavone-mediated autophagy and neuroprotection in vitro. Conclusion: Isoflavones activate BEX2-dependent autophagy, protecting against ATR-induced neuronal apoptosis.

show abstract

Resveratrol Partially Prevents Rotenone-Induced Neurotoxicity in Dopaminergic SH-SY5Y Cells through Induction of Heme Oxygenase-1 Dependent Autophagy

Cited by 144 publications

References 66 publications

Sesamol Antagonizes Rotenone-Induced Cell Death in Sh-Sy5y Neuronal Cells

Sesamol Antagonizes Rotenone-Induced Cell Death in Sh-Sy5y Neuronal Cells

Resveratrol ameliorates experimental periodontitis in diabetic mice through negative regulation of TLR4 signaling

Isoflavones Induce BEX2-Dependent Autophagy to Prevent ATR-Induced Neurotoxicity in SH-SY5Y Cells

Contact Info

Product

Resources

About