Resveratrol mimics insulin activity in the adipogenic commitment of human bone marrow mesenchymal stromal cells

Caldarelli, Ilaria; Speranza, Maria C.; Bencivenga, Debora; Tramontano, Annunziata; Borgia, Alessia; Pirozzi, Anna Virginia Adriana; Perrotta, Silverio; Oliva, Adriana; Ragione, Fulvio Della; Borriello, Adriana

doi:10.1016/j.biocel.2014.12.011

“…Along with anti‐inflammatory effects, polyphenols also have an impact on diabetic control by improving insulin resistance by inducing the transcriptional activation of peroxisome proliferator activated receptor‐γ and increasing adiponectin . Among polyphenols, resveratrol gained great interest in terms of insulin resistance as shown by Caldarelli et al They reported that resveratrol mimicked insulin and modulated adipogenesis in type 2 diabetes. In a recent study, GSPE was also found to regulate insulin resistance and have a positive long‐term effect (25 mg/kg body weight per day of GSPE 30 days) on glucose homeostasis in hyperinsulinemic animals (fed a cafeteria diet) .…”

Section: Discussionmentioning

confidence: 99%

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Toker

¹

,

Yüce

²

,

Alpan

³

et al. 2018

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

“…The application of resveratrol to mesenchymal stem cell-based regenerative medicine has been demonstrated in various in vitro bioactivities, including self-renewal, multipotency (33), senescence (21), cell aging (34), osteogenic differentiation (35), adipogenic differentiation (36), and neuronal differentiation (12). Moreover, resveratrol has an e ciently therapeutic effect on in vivo models, including enhancing liver regeneration (37), cardiogenic differentiation in cardiomyopathy (38).…”

Section: Discussionmentioning

confidence: 99%

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Songsaad

¹

,

Gonmanee

²

,

Ruangsawasdi

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Introduction: Stem cell transplantation of exogenous neural progenitor cells (NPCs) derived from mesenchymal stem cells (MSCs) has emerged as a promising approach of neurodegenerative disease. Human stem cells from apical papilla (hSCAPs) are derived from migratory neural crest stem cells, and exhibit a potential of neuronal differentiation. However, their neuronal differentiation is low and unpredictable. Resveratrol has been described as a sirtuin 1 (SIRT1) activator which plays an important role in enhancing neuronal differentiation. In this study, we investigate the potential of resveratrol as an enhancer on neuronal differentiation through NPCs induction of hSCAPs.Methods: Stem cells were isolated from human apical papilla, and characterized as MSCs. The cellular toxicity of resveratrol treatment to the characterized hSCAPs was investigated by MTT assay. The non-cellular toxicity concentrations of resveratrol were assessed with various pre-treatment times to select the optimal condition that highly expressed the neural progenitor gene, NES. Consequently, the optimal condition of resveratrol pre-treatment was synergistically performed with a neuronal induction medium to trigger neuronal differentiation. The differentiated cells were visualized, the genes profiling was quantified, and the percentage of neuronal differentiation was calculated. Moreover, the intracellular calcium oscillation was demonstrated. Results: The cellular toxicity of resveratrol was not observed for up to 50 µM for 12 hours. Interestingly, hSCAPs pre-treated with 10 µM resveratrol for 12 hours (RSV-hSCAPs) significantly expressed NES, which is determined as the optimal condition. Under neuronal induction, both of hSCAPs and RSV-hSCAPs were differentiated (d-hSCAPs, and RSV-d-hSCAPs) as they exhibited neuronal-like appearances with Nissl substance staining. The highest expression of NES, and SOX1 was observed in RSV-d-hSCAPs. Additionally, the percentage of neuronal differentiation of RSV-d-hSCAPs was significantly higher than d-hSCAPs for 4 times. Importantly, the neuronal-like cells exhibited slightly increasing pattern of calcium intensity.Conclusion: This study demonstrated that pre-treatment of resveratrol strongly induces neural progenitor marker gene expression which synergistically enhances neural progenitor-like cells’ induction with neuronal induction medium.

show abstract

“…The application of resveratrol to mesenchymal stem cell-based regenerative medicine has been demonstrated in various in vitro bioactivities, including self-renewal, multipotency (45), senescence (21), cell aging (46), osteogenic differentiation (47), adipogenic differentiation (48), and neuronal differentiation (12). Importantly, in vitro model have potential to demonstrate insight into cellular and molecular mechanism.…”

Section: Discussionmentioning

confidence: 99%

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Songsaad

¹

,

Gonmanee

²

,

Ruangsawasdi

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Introduction: Stem cell transplantation of exogenous neural progenitor cells (NPCs) derived from mesenchymal stem cells (MSCs) has emerged as a promising approach of neurodegenerative disease. Human stem cells from apical papilla (hSCAPs) are derived from migratory neural crest stem cells, and exhibit a potential of neuronal differentiation. However, their neuronal differentiation is low and unpredictable. Resveratrol has been described as a sirtuin 1 (SIRT1) activator which plays an important role in enhancing neuronal differentiation. In this study, we investigate the potential of resveratrol as an enhancer on neuronal differentiation through NPCs induction of hSCAPs.Methods: Stem cells were isolated from human apical papilla, and characterized as MSCs. The cellular toxicity of resveratrol treatment to the characterized hSCAPs was investigated by MTT assay. The non-cellular toxicity concentrations of resveratrol were assessed with various pre-treatment times to select the optimal condition that highly expressed the neural progenitor gene, NES. Consequently, the optimal condition of resveratrol pre-treatment was synergistically performed with a neuronal induction medium to trigger neuronal differentiation. The differentiated cells were visualized, the genes profiling was quantified, and the percentage of neuronal differentiation was calculated. Moreover, the intracellular calcium oscillation was demonstrated. Results: The cellular toxicity of resveratrol was not observed for up to 50 µM for 12 hours. Interestingly, hSCAPs pre-treated with 10 µM resveratrol for 12 hours (RSV-hSCAPs) significantly expressed NES, which is determined as the optimal condition. Under neuronal induction, both of hSCAPs and RSV-hSCAPs were differentiated (d-hSCAPs, and RSV-d-hSCAPs) as they exhibited neuronal-like appearances with Nissl substance staining. The highest expression of NES, and SOX1 was observed in RSV-d-hSCAPs. Additionally, the percentage of neuronal differentiation of RSV-d-hSCAPs was significantly higher than d-hSCAPs for 4 times. Importantly, the neuronal-like cells exhibited slightly increasing pattern of calcium intensity.Conclusion: This study demonstrated that pre-treatment of resveratrol strongly induces neural progenitor marker gene expression which synergistically enhances neural progenitor-like cells’ induction with neuronal induction medium.

show abstract

Resveratrol mimics insulin activity in the adipogenic commitment of human bone marrow mesenchymal stromal cells

Cited by 24 publications

References 67 publications

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Contact Info

Product

Resources

About