Resveratrol attenuates myocardial hypoxia/reoxygenation-induced cell apoptosis through DJ-1-mediated SIRT1-p53 pathway

Xu, Ruiyuan; Xu, Xing-Wang; Deng, Yi-Zhang; Ma, Zhaoxia; Li, Xiaoran; Zhao, Le; Qiu, Le-Jia; Liu, Haoyue; Chen, Heping

doi:10.1016/j.bbrc.2019.04.165

Cited by 30 publications

(22 citation statements)

References 31 publications

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“…Moreover, Sirt1 knockdown by siRNAs markedly abrogated the inhibitory effect of PSPC on PA-induced apoptosis in L02 cells. Consistent with these studies (12,23,35), our results revealed that PSPC might exhibit inhibitory effects on HFD-induced hepatic apoptosis via restoring Sirt1 level.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, HFD markedly elevated the expression of P53 and its acetylation at Lys379 in the mouse livers. P53 is identified as a key downstream target of Sirt1, and its transcriptional activity was restrained by SirT1-dependent deacetylation (12,38). Therefore, our findings indicated that HFD might trigger p53dependent hepatic apoptosis by down-regulating Sirt1.…”

Section: Discussionmentioning

confidence: 64%

“…In this study, HFD remarkably reduced the protein expression of SirT1 as well as its activity in mouse livers, indicating that HFD-induced hepatic apoptosis may be associated with the down-regulation of Sirt1. It has been widely reported that some naturally occurring polyphenols, such as resveratrol, are Sirt1 activators, which exhibit beneficial effects on a variety of diseases including NAFLD (12,35). Our previous study also demonstrated that troxerutin, a flavonoid, markedly renewed Sirt1 levels to protect mouse liver from HFD-induced steatosis (23).…”

Section: Discussionmentioning

confidence: 84%

“…It has been well established that Sirt1 plays a critical role in apoptosis inhibition under various pathological conditions (11,12,34). A growing body of evidence suggests that Sirt1 is down-regulated in diverse tissue injuries and diseases, which promotes apoptosis to cause tissue damage (11,12,34).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the NAD + depletionmediated down-regulation of Sirt1 is emerging as a major contributor to the pathogenesis of various diseases including T2D, which contributes to many disorders of these diseases, such as oxidative stress, mitochondrial damage and inflammation (9,10). It has been well demonstrated that SirT1 plays a crucial role in the suppression of cell apoptosis during multiple physiological and pathological processes, owing to its ability to deacetylate numerous substrates involving in various apoptotic and survival pathway, such as p53 and NF-κB (11,12). Accumulating evidence indicates that SirT1 is involved in regulating hepatocyte apoptosis during NAFLD (7,13).…”

mentioning

confidence: 99%

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Purple sweet potato color protects against hepatocyte apoptosis through Sirt1 activation in high-fat-diet-treated mice

Su¹,

Zhang²,

Chen³

et al. 2020

Food & Nutrition Research

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Background: Recent evidence indicates that the inhibition of hepatocyte apoptosis is possible to develop a potential therapeutic strategy for nonalcoholic fatty liver disease (NAFLD). Our previous work suggested that purple sweet potato color (PSPC), a class of naturally occurring anthocyanins, effectively improved many features of high-fat diet (HFD)-induced NAFLD. However, whether PSPC ameliorates HFD-induced hepatocyte apoptosis has never been investigated. Objective: Here we investigated the effects of PSPC on HFD-induced hepatic apoptosis and the mechanisms underlying these effects. Design: Mice were divided into four groups: Control group, HFD group, HFD + PSPC group and PSPC group. PSPC was administered by daily oral gavage at doses of 700 mg/kg/day for 20 weeks. EX-527 (a SirT1-selective inhibitor) and Sirt1 siRNA were used to demonstrate the Sirt1 dependence of PSPC-mediated effects on apoptotic and survival signaling pathways in vivo and in vitro. Results: Our results showed that PSPC reduced body weights, hepatic triglyceride contents, histopathological lesions and serum ALT levels in a mouse model of NAFLD induced by HFD. Furthermore, PSPC attenuated HFD-induced hepatocyte apoptosis ratio from 7.27 ± 0.92% to 1.79 ± 0.27% in mouse livers, which is insignificant compared with that of controls. Moreover, PSPC activated Sirt1 by boosting NAD + level in HFD-treated mouse livers. Furthermore, PSPC promoted Sirt1-dependent suppression of P53-mediated apoptotic signaling and activation of Akt survival signaling pathway in HFD-treated mouse livers, which was confirmed by EX527 treatment. Moreover, Sirt1 knockdown abolished these ameliorative effects of PSPC on apoptosis and P53 acetylation and protein expression in PA-treated L02 cells. Ultimately, PSPC reduced Caspase-3 activation and Bax level, and elevated the Bcl-2 level in HFD-treated mouse livers. Conclusion: PSPC protected against HFD-induced hepatic apoptosis by promoting Sirt1-dependent inhibition of p53-apoptotic pathway and facilitation of Akt survival pathway. This study indicates that PSPC is a candidate for nutritional intervention of NAFLD.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Purple sweet potato color protects against hepatocyte apoptosis through Sirt1 activation in high-fat-diet-treated mice

Su¹,

Zhang²,

Chen³

et al. 2020

Food & Nutrition Research

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Garcinol protects SH‐SY5Y cells against MPP+‐induced cell death by activating DJ‐1/SIRT1 and PGC‐1α mediated antioxidant pathway in sequential stimulation of p‐AMPK mediated autophagy

Lee

Kumar

Huang

et al. 2023

Environmental Toxicology

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Parkinson's disease (PD), a chronic and progressive neurodegenerative disease, can reduce the population of dopaminergic neurons in the substantia nigra. The cause of this neuronal death remains unclear. 1-Methyl-4-phenylpyridinium ion (MPP+) is a potent neurotoxin that can destroy dopaminergic (DA) neurons and promote PD. Garcinol, a polyisoprenylated benzophenone derivative, was extracted from Garcinia indica and is an important active compound it has been used as an anticancer, antioxidant, and anti-inflammatory, agent and it can suppress reactive oxygen species (ROS) mediated cell death in a PD model. Human neuroblastoma (SH-SY5Y) cells (1 Â 10 5 cells) were treated with MPP+ (1 mM) for 24 h to induce cellular ROS production. The formation of ROS was suppressed by pretreatment with different

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Naringin against doxorubicin‐induced hepatotoxicity in mice through reducing oxidative stress, inflammation, and apoptosis via the up‐regulation of SIRT1

Yan

Chi

Tao

et al. 2023

Environmental Toxicology

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Clinical application of doxorubicin is limited because of its potential side effects. The present study examined whether naringin had protective actions on doxorubicin‐induced liver injury. Male BALB/c mice and alpha mouse liver 12 (AML‐12) cells were used in this paper. The results showed that AML‐12 cells treated with naringin significantly reduced cell injury, reactive oxygen species release and apoptosis level; Moreover, naringin notably alleviated liver injury by decreasing aspartate transaminase, alanine transaminase and malondialdehyde, and increasing superoxide dismutase, glutathione and catalase levels. Mechanism researches indicated that naringin increased the expression levels of sirtuin 1 (SIRT1), and inhibited the downstream inflammatory, apoptotic and oxidative stress signaling pathways. Further validation was obtained by knocking down SIRT1 in vitro, which proved the effects of naringin on doxorubicin‐induced liver injury. Therefore, naringin is a valuable lead compound for preventing doxorubicin‐induced liver damage by reducing oxidative stress, inflammation, and apoptosis via up‐regulation of SIRT1.

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Resveratrol attenuates myocardial hypoxia/reoxygenation-induced cell apoptosis through DJ-1-mediated SIRT1-p53 pathway

Cited by 30 publications

References 31 publications

Purple sweet potato color protects against hepatocyte apoptosis through Sirt1 activation in high-fat-diet-treated mice

Purple sweet potato color protects against hepatocyte apoptosis through Sirt1 activation in high-fat-diet-treated mice

Garcinol protects SH‐SY5Y cells against MPP+‐induced cell death by activating DJ‐1/SIRT1 and PGC‐1α mediated antioxidant pathway in sequential stimulation of p‐AMPK mediated autophagy

Naringin against doxorubicin‐induced hepatotoxicity in mice through reducing oxidative stress, inflammation, and apoptosis via the up‐regulation of SIRT1

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