Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells

Bae, Seunghee; Lee, Eun Mee; Jun, Hwa; Kim, Karam; Yoon, Younju; Lee, Hyunjin; Kim, Jongran; Kim, Yu Jeong; Lee, Hong Ghi; Jeung, Hoi Kyung; Min, Yoo Hong; An, Sungkwan

doi:10.1007/s10059-011-1037-z

Cited by 86 publications

(55 citation statements)

References 37 publications

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“…Besides, cell cyclin involving E1, D1, MMP2 and MMP9 is significantly changed in cell transfected with miR‐139‐5p mimics 25, 46. Furthermore, a decrease in the apoptotic protein such as Bax 47, 48 and an increase in the anti‐apoptotic protein including Bcl‐2 as well as Mcl‐1 47, 49, regulated by miR‐139‐5p, also participates in the tumour process. In the present study, miR‐139‐5p was found down‐regulated in the OSCC sample tissues, and its up‐regulation suppressed the viability, proliferation, migration and invasiveness of OSCC cells.…”

Section: Discussionmentioning

confidence: 99%

MiR‐139‐5p inhibits the tumorigenesis and progression of oral squamous carcinoma cells by targeting HOXA9

Wang

Jin

et al. 2017

J Cellular Molecular Medi

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Our study sought to clarify the effects of microRNA‐139‐5p (miR‐139‐5p) in the tumorigenesis and progression of oral squamous cell carcinoma (OSCC) by regulating HOXA9. MiR‐139‐5p and HOXA9 expression in OSCC tissues, tumour adjacent tissues, OSCC cells and normal cells were tested by qRT‐PCR. SAS and CAL‐27 cell lines were selected in among four OSCC cell lines and then transfected with miR‐139‐5p mimics, pEGFP‐HOXA9 and cotransfected with miR‐139‐5p mimics + pEGFP‐HOXA9. We used MTT, colony formation, transwell and wound healing assays to analyse cell viability, proliferation, invasion and migration. The target relationship between miR‐139‐5p and HOXA9 was verified by luciferase reporter assay and Western blot, respectively. MiR‐139‐5p was down‐regulated, whereas HOXA9 was up‐regulated in OSCC tissues and cells. The proliferation, invasion and migration ability of SAS and CAL‐27 cells in miR‐139‐5p mimics group were significantly weaker than those in the control group and the miR‐NC group (P < 0.01). MiR‐139‐5p can negatively regulate HOXA9. The proliferation, invasion and migration of SAS and CAL‐27 cells in the miR‐139‐5p mimics + pEGFP‐HOXA9 group were not significantly different from those in the blank control and negative control groups (P > 0.05). Our results indicated that miR‐139‐5p could directly inhibit HOXA9, which might be a potential mechanism in inhibiting the proliferation, invasiveness and migration of OSCC cells.

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Section: Discussionmentioning

confidence: 99%

MiR‐139‐5p inhibits the tumorigenesis and progression of oral squamous carcinoma cells by targeting HOXA9

Wang

Jin

et al. 2017

J Cellular Molecular Medi

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“…9,10 Resveratrol has been shown to induce apoptosis in a variety of cancers such as chronic myeloid leukemia (CML), acute myeloid leukemia (AML), breast, prostate, colon and lung cancers. [11][12][13] The exact mechanism of resveratrol mediated anticancer effect is not fully identified.…”

Section: Introductionmentioning

confidence: 99%

“…As indicated in several studies, resveratrol-induced apoptotic mechanisms include inhibition of retinoblastoma (Rb) phosphorylation, suppression of NF-κB and STAT3, induction of p21 11,14 and ceramide synthases and inhibition of sphingosine kinase-1 and glucosyle ceramide synthase. 12,13 It has been also shown that resveratrol inhibits the cell cycle progression by causing arrest at G1/G2 phases.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

2013

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Chronic lymphocytic leukemia (CLL), defined by accumulation of pathogenic B cells, has a very complex biology due to various factors such as inherited, host, and enviromental factors. Recently, finding new therapeutic agents or development of novel treatment strategies have been paid attention. Resveratrol and quercetin, important phytoalexins found in many plants, have been reported to have cytotoxic effects on various types of cancer. In this study, we examined cytotoxic, cytostatic, and apoptotic effects of these two important phenolic compounds on 232B4 human CLL cells. Cytotoxic effects of resveratrol and quercetin were determined by MTT cell proliferation assay. Changes in caspase-3 enzyme activity were measured using caspase-3 colorimetric assay. Annexin V-FITC/PI double staining was performed to measure apoptotic cell population. Effects of resveratrol and quercetin on cell cycle profiles of CLL cells were investigated by flow cytometry. Treatment of CLL cells with resveratrol and quercetin caused dose dependent inhibition of cell proliferation and increased apoptotic cell population through induction of caspase-3 activity. Cell cycle analysis displayed cell cycle arrest mainly in G0/G1 for both polyphenols. Our data, in total, showed for the first time that resveratrol and quercetin might block CLL growth through inducing apoptosis and cell cycle arrest.

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“…48 miR34a was modulated by resveratrol in A549 human non-small cell lung cancer (NSCLC) cells. 49 This treatment reduced the expression of oncogenic miRNAs and induced pro-apoptotic miRNAs in colon cancer cells. 50,51 Two flavonoids, rhamnetin and cirsiliol, upregulated p53-dependent miR-34a expression in NSCLC cells, resulting in radiosensitization through increased apoptosis.…”

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confidence: 99%

Dietary flaxseed modulates the miRNA profile in irradiated and non-irradiated murine lungs

Christofidou‐Solomidou

Pietrofesa

Arguiri

et al. 2014

Cancer Biology & Therapy

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Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells

Cited by 86 publications

References 37 publications

MiR‐139‐5p inhibits the tumorigenesis and progression of oral squamous carcinoma cells by targeting HOXA9

MiR‐139‐5p inhibits the tumorigenesis and progression of oral squamous carcinoma cells by targeting HOXA9

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

Dietary flaxseed modulates the miRNA profile in irradiated and non-irradiated murine lungs

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