Resveratrol alleviates lysophosphatidylcholine-induced damage and inflammation in vascular endothelial cells

Abstract

The role of resveratrol (trans-3,5,4'-trihydroxystilbene; RES) in lysophosphatidylcholine (LPC)‑induced injury and inflammation in endothelial cells (regarded as an early event in arteriosclerosis) is unclear. The present study investigated whether RES reduces lactate dehydrogenase (LDH) activity and secretion of inflammatory cytokines such asinterleukin‑6 and tumor necrosis factor‑α, via the Toll‑like receptor (TLR)‑4/myeloid differentiation primary response gene 88 (MyD88)/nuclear factor (NF)‑κB signal transduction pathway in LPC‑induced damage and inflammation in human umbilical vein endothelial‑12 (HUVE‑12) cells. Using an ELISA and western blotting, the present study investigated the effects of RES on LDH activity and cytokine secretion. The effects of TLR‑4 short hairpin (sh)RNA and TLR‑4 cDNA transfection on NF‑κB activation during LPC‑induced damage and inflammation was also investigated in HUVE‑12 cells. The results demonstrated that RES significantly inhibited the effect of LPC on enzyme activity, pro‑inflammatory cytokine secretion, and expression of TLR‑4, MyD88 and NF‑κBp65 expression. In addition, RES and TLR‑4 shRNA transfection suppressed LPC‑induced injury and inflammation by blocking the TLR‑4/MyD88/NF‑κB signaling pathway Conversely, transfection with TLR‑4 cDNA enhanced LPC‑induced injury and inflammation, which abrogated the protective effects of RES. These data suggested that RES significantly suppressed LPC‑induced damage and inflammation, via suppression of the TLR‑4/MyD88/NF‑κB signaling pathway, which may provide a new mechanistic evidence for the treatment of arteriosclerosis by RES.

Effect of Resveratrol on Blood Lipid Levels in Patients with Type 2 Diabetes: A Systematic Review and Meta‐Analysis

“…Resveratrol is found in grapes, peanuts, cranberries, and many other foods including Japanese knotweed ( Polygonum cuspidatum ), which is particularly high in resveratrol and can be used as herb. Studies have shown that resveratrol has a wide range of effects, including antimicrobial , anti‐inflammatory , antiapoptotic , and anticancer effects, and promotes osteogenic factor formation . Resveratrol can also play a beneficial role in liver diseases and hepatic steatosis by reducing oxidative stress and hepatic fibrosis .…”

Effect of resveratrol on growth performance, rectal temperature and serum parameters of yellow‐feather broilers under heat stress

“…Taken together, we speculated that the beneficial effects of resveratrol on heat‐stressed broilers, at least partially ascribed to the antioxidant capacity and hepato‐protective effect, and the high‐dose resveratrol showed better results, it possible be the lower dose of 200 mg/kg resveratrol not afford to neutralize heat stress damage. Previous researches demonstrated that resveratrol decreased activities of LDH in lipopolysaccharide‐induced stress vascular endothelial cells and via suppression of the Toll‐like receptor 4/myeloid differentiation factor88/nuclear factor‐kappaB signaling pathway suppress tissue damage (Chen et al., ), and decreased activities of LDH improved muscle quality of transport‐stressed broilers (Zhang et al., ). Das () also reported that supplementation with resveratrol decreased the activities of ALT and AST, and had shown to be an effective antidote in heat‐stressed rats.…”

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

“…A recent clinical trial involving primary hypertensive patients evidenced that addition of a micronized formulation of RSV to standard antihypertensive therapy is sufficient to normalize the blood pressure, without additional antihypertensive drugs [127]. Other targets of RSV were identified by different other groups: TLR4 [128,129], miR-221/222 [130] and p38 MAPK [131,132].…”

“…RSV induces the decrease of endothelial activation and vascular inflammation, and improves the endothelial function. It was demonstrated that RSV determines the decrease of IL-6 and TNF-α via the TLR4/myeloid differentiation primary response gene 88 (MyD88)/NF-κB signal transduction pathway in HUVECs exposed to LPS [128]. In addition, it was reported that pre-incubation with RSV reduced the TNF-α-induced ICAM-1 secretion, as well as the intracellular expression of ICAM-1 and MMP-9 in EC by inducing autophagy, mediated in part through the activation of the cAMP/protein kinase A (PKA)/AMPK/SIRT-1 signaling pathway [133].…”