Results of an interlaboratory test on antimicrobial susceptibility testing of bacteria from animals by broth microdilution

Wallmann, Jürgen; Böttner, A.; Goossens, L.; Hafez, H. M.; Hartmann, Katrin; Kaspar, Heike; Kehrenberg, Corinna; Kietzmann, Manfred; Klarmann, Dieter; Klein, Günter; Krabisch, Peter; Kühn, Tilman; Luhofer, Gabriele; Richter, Angelika; Schulz, Bianka; Schwarz, Štefan; Sigge, Claudia; Traeder, Wolfgang; Waldmann, Karl-Heinz; Werckenthin, Christiane Susanne; Zschiesche, E.

doi:10.1016/j.ijantimicag.2005.12.011

Cited by 17 publications

(10 citation statements)

References 14 publications

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“…The reproducibility of the broth micro-dilution method used in this study for determining the antibiotics MIC values has been confirmed in previous studies and is one of National Committee for Clinical Laboratory Standards (NCCLS) recommended methods for determining antibiotic MIC values [41,46]. Our results showed that the investigated strains were resistant to high concentration of vancomycin.…”

Section: Discussionsupporting

confidence: 82%

Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products

et al. 2012

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BackgroundIndigenous fermented food products play an essential role in the diet of millions of Africans. Lactic acid bacteria (LAB) are among the predominant microbial species in African indigenous fermented food products and are used for different applications in the food and biotechnology industries. Numerous studies have described antimicrobial susceptibility profiles of LAB from different parts of the world. However, there is limited information on antimicrobial resistance profiles of LAB from Africa. The aim of this study was to characterize 33 LAB previously isolated from three different African indigenous fermented food products using (GTG)5-based rep-PCR, sequencing of the 16S rRNA gene and species-specific PCR techniques for differentiation of closely related species and further evaluate their antibiotic resistance profiles by the broth microdilution method and their haemolytic activity on sheep blood agar plates as indicators of safety traits among these bacteria.ResultsUsing molecular biology based methods and selected phenotypic tests such as catalase reaction, CO2 production from glucose, colonies and cells morphology, the isolates were identified as Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus ghanensis, Lactobacillus plantarum, Lactobacillus salivarius, Leuconostoc pseudomesenteroides, Pediococcus acidilactici, Pediococcus pentosaceus and Weissella confusa. The bacteria were susceptible to ampicillin, chloramphenicol, clindamycin and erythromycin but resistant to vancomycin, kanamycin and streptomycin. Variable sensitivity profiles to tetracycline and gentamicin was observed among the isolates with Lb. plantarum, Lb. salivarius, W. confusa (except strain SK9-5) and Lb. fermentum strains being susceptible to tetracycline whereas Pediococcus strains and Lb. ghanensis strains were resistant. For gentamicin, Leuc. pseudomesenteroides, Lb. ghanensis and Ped. acidilactici strains were resistant to 64 mg/L whereas some W. confusa and Lb. plantarum strains had a MIC value of 16 mg/L and 32 mg/L respectively. No β-haemolytic activity was observed, however, α-haemolytic activity was observed in 27% (9) of the strains comprising Lb. salivarius (6), W. confusa (2) and Lb. delbrueckii (1) isolates.ConclusionsThe resistance to kanamycin and vancomycin is probably an intrinsic feature since similar observations were reported in the literature for LAB. Low prevalence of pathogenicity indicator traits were observed among the isolates especially with the presence of poor haemolytic activities and they could therefore be considered as interesting candidates for selection of starter cultures or probiotics for different applications.

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Section: Discussionsupporting

confidence: 82%

Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products

et al. 2012

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“… T > MIC has been calculated for MICs of 0.06, 0.125, and 0.39 μg/ml on the basis of reported MIC 90s (0.06–0.39 μg/ml) for Escherichia coli , Pasteruella multocida, and Streptococcus agalactiae (Chin et al., ; Deshpande et al., ; Limbert et al., ; Murphy et al., ; Orden et al., ; Sheldon et al., ; Thomas et al., ; Wallmann et al., ). …”

Section: Pharmacokinetic Parameters (Mean ± Sd) Of Cefquinome In Buffmentioning

confidence: 99%

“…It is generally recommended that T > MIC should be at least 50% of the dosage interval to ensure an optimal bactericidal effect (Toutain & Lees, ). Considering the reported MIC 90s (0.06–0.39 μg/ml) for Escherichia coli , Pasteruella multocida, and Streptococcus agalactiae (Chin, Gu, Fang, & Neu, ; Deshpande, Pfaller, & Jones, ; Limbert et al., ; Murphy, Erwin, & Jones, ; Orden, Ruiz, Garcia, Cid, & Fuente, ; Sheldon, Bushnell, Montgomery, & Rycroft, ; Thomas et al., ; Wallmann et al., ), the T > MIC has been calculated for MICs of 0.06, 0.125, and 0.39 μg/ml. The experimental data presented here show that cefquinome at a dose of 2 mg/kg body weight at 24 hr interval is sufficient to maintain T > MIC above 58% following IM injection, respectively, for bacteria with MIC values ≤0.39 μg/ml.…”

Section: Pharmacokinetic Parameters (Mean ± Sd) Of Cefquinome In Buffmentioning

confidence: 99%

“…It is generally recommended that T > MIC should be at least 50% of the dosage interval to ensure an optimal bactericidal effect (Toutain & Lees, 2004). Considering the reported MIC 90s (0.06-0.39 μg/ml) for Escherichia coli, Pasteruella multocida, and Streptococcus agalactiae (Chin, Gu, Fang, & Neu, 1992;Deshpande, Pfaller, & Jones, 2000;Limbert et al, 1991;Murphy, Erwin, & Jones, 1994;Orden, Ruiz, Garcia, Cid, & Fuente, 1999;Sheldon, Bushnell, Montgomery, & Rycroft, 2004;Thomas et al, 2006;Wallmann et al, 2006) Rapid absorption and high bioavailability of cefquinome indicate that IM injection is as effective as IV injection in the treatment of mildto-moderate bacterial infections in buffalo species. The suggested dosage regimen has to be validated in the diseased model.…”

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confidence: 99%

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Pharmacokinetics of a single intramuscular injection of cefquinome in buffalo calves

Venkatachalam

Dumka

Ranjan

2017

Vet Pharm & Therapeutics

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The objective of this study was to investigate the pharmacokinetics of cefquinome following single intramuscular (IM) administration in six healthy male buffalo calves.Cefquinome was administered intramuscularly (2 mg/kg bodyweight) and blood samples were collected prior to drug administration and up to 24 hr after injection. No adverse effects or changes were observed after the IM injection of cefquinome.Plasma concentrations of cefquinome were determined by high-performance liquid chromatography. The disposition of plasma cefquinome is characterized by a monocompartmental open model. The pharmacokinetic parameters after IM administration (mean ± SE) were C max 6.93 ± 0.58 μg/ml, T max 0.5 hr, t ½kα 0.16 ± 0.05 hr, t ½β 3.73 ± 0.10 hr, and AUC 28.40 ± 1.30 μg hr/ml after IM administration. A dosage regimen of 2 mg/kg bodyweight at 24-hr interval following IM injection of cefquinome would maintain the plasma levels required to be effective against the bacterial pathogens with MIC values ≤0.39 μg/ml. The suggested dosage regimen of cefquinome has to be validated in the disease models before recommending for clinical use in buffalo calves.

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“…For this purpose, evaluation of the internal and external quality assurance procedures for reference methods and commercial systems for susceptibility testing is absolutely crucial for the correct interpretation of these ECOFFS. Several harmonization studies of susceptibility testing of clinical (14,30), veterinary (26,34), and aquatic (11,25) organisms have provided important insights into the reproducibility of results from standardized methods at the intra-and/or interlaboratory level. Although such studies would also be highly valuable to all with an interest in evidence-based biosafety assessments of NELAB and bifidobacteria for human and animal use, the performances of susceptibility test methods using LSM within and across laboratories have to our knowledge not been evaluated.…”

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confidence: 99%

Intra- and Interlaboratory Performances of Two Commercial Antimicrobial Susceptibility Testing Methods for Bifidobacteria and Nonenterococcal Lactic Acid Bacteria

Huys

D'Haene

Cnockaert

et al. 2010

Antimicrob Agents Chemother

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In a small-scale harmonization study involving nine laboratories in eight European countries, the intra-and interlaboratory performances of two commercially available systems, i.e., the VetMIC microplate system and Etest, for antimicrobial susceptibility testing of nonenterococcal lactic acid bacteria (NELAB) and bifidobacteria were analyzed. In addition, one laboratory also performed standard broth microdilution as a reference method. MICs of tetracycline, erythromycin, ampicillin, gentamicin, clindamycin, and streptomycin for the type strains of 25 species of NELAB and bifidobacteria and MICs of vancomycin for a selection of relevant taxa were determined. The previously described lactic acid bacterium susceptibility test medium (LSM) and related mixed-medium formulations, all including Iso-Sensitest broth as a basic component, were used as test media. The overall agreement of median MIC ranges ؎ 1 log 2 dilution determined by the VetMIC and Etest methods with the median MICs determined by the reference method was very good for tetracycline, ampicillin, and streptomycin (92.3 to 100%) but low for erythromycin (19.5 to 30.7%) and clindamycin (50.0 to 80.8%). There was a consensus among the participating laboratories that VetMIC was preferred over Etest because of its lower cost, better growth support, and more uniform criteria for MIC end point reading. With the range for acceptable intralaboratory reproducibility being defined as the median MIC ؎ 1 log 2 dilution, VetMIC results (with 69.2% of all data sets in the acceptable range) were shown to display greater reproducibility than Etest results (with 58.8% of all data sets in the acceptable range). Also at the interlaboratory level, the proportion of MIC values obtained with VetMIC that belonged to the complete agreement category (60.0%) was higher than the proportion of such values obtained with Etest (47.0%), which indicates a higher degree of interlaboratory reproducibility for the former method. Apart from some agent-specific effects, the majority of VetMIC and Etest replicate data sets were situated within a 1-to 2-log 2 dilution range, suggesting that the two methods can be considered to be equivalent for recognizing resistance phenotypes. This multicenter study has further validated the standard use of LSM and related mixed-medium formulations with commercially available systems and formed the basis for the ongoing development of the ISO 10932/IDF 223 standard for susceptibility testing of NELAB and bifidobacteria.

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Results of an interlaboratory test on antimicrobial susceptibility testing of bacteria from animals by broth microdilution

Cited by 17 publications

References 14 publications

Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products

Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products

Pharmacokinetics of a single intramuscular injection of cefquinome in buffalo calves

Intra- and Interlaboratory Performances of Two Commercial Antimicrobial Susceptibility Testing Methods for Bifidobacteria and Nonenterococcal Lactic Acid Bacteria

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