Restraint effects on stress-related hormones and blood natural killer cell cytotoxicity in pigs with a mutated ryanodine receptor

Ciepielewski, Z.; Stojek, W.; Glac, W.; Wrona, Danuta

doi:10.1016/j.domaniend.2013.02.003

Cited by 11 publications

(7 citation statements)

References 45 publications

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“…Though short-term exposure to CORT has been shown to exert a synergistic effect with cytokines like IL-15 in promoting natural killer (NK) cell function, 72 sustained exposure results in a decrease in NK cell response. 73 This alignment with a natural homeostatic attractor suggests that NK cell functional deficits might be a natural regulatory response in this context instead of a primary injury to immune function. If this is the case then these predictions would also imply that male GWI and CFS subjects may be resistant to NK cell promoters used alone, that their effects will not be long lasting and continued long-term treatment courses may be necessary unless combined with other treatment components.…”

Section: Discussionmentioning

confidence: 99%

Succumbing to the laws of attraction

Fritsch

Craddock

Rosario

et al. 2013

Systems Biomedicine

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systems Biomedicine Volume 1 Issue 3 PF, TJAC, RMdR, and MAR performed computational simulations and analysis of computational results. PF, TJAC, ALS, VAF, and GB, compiled physiological data and developed the immune-endocrine connectivity network. PF, TJAC, and GB performed comparisons between experimental and computational data and interpreted the results. MAF and NGK collected and analyzed the experimental data. TJAC, RMdR, MAR, GdV, and GB conceived of the discrete logical analysis algorithm. All authors contributed to the drafting of this article, and approve of its contents.

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Section: Discussionmentioning

confidence: 99%

Succumbing to the laws of attraction

Fritsch

Craddock

Rosario

et al. 2013

Systems Biomedicine

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“…Even though the increase of GCs and CAs upon stressor exposure is well documented in pigs, only a few experiments have studied the functionality of immune cells under the influence of stress hormones in this important livestock species so far. It was shown, for example, that social isolation, weaning, restraint or regrouping led to an increase in endogenic cortisol production, thus resulting in the suppression of lymphocyte proliferation [16,17,22,23,24] and a reduced expression of pro-inflammatory cytokines [21,25,26]. However, it is likely that these immune-modulating effects cannot solely be attributed to cortisol, as a concurrent activation of the sympathetic nervous system (SNS) which leads to the secretion of ADR and NA is probable.…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoids and Catecholamines Affect in Vitro Functionality of Porcine Blood Immune Cells

Reiske

Schmucker

Steuber

et al. 2019

Animals

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Stress hormones exert important modulating influences on the functionality of immune cells. Despite its major role as a livestock animal and its increasing use as an animal model, knowledge about this relationship in the domestic pig is rare. This study therefore aimed to characterize the effect of glucocorticoids and catecholamines on the proliferation and cytokine production of porcine peripheral blood mononuclear cells (PBMC). Blood was obtained from donor pigs equipped with indwelling catheters to exclude stress hormone exposition before in vitro testing. PBMC were stimulated in the presence of cortisol, adrenaline or noradrenaline at concentrations resembling low to high stress conditions. Proliferation was determined via 3H-thymidine incorporation, and TNFα producers were quantified by intracellular cytokine staining. Cortisol led to a decrease in mitogen-induced lymphocyte proliferation and the number of TNFα producing cells. In contrast, catecholamines increased proliferation while exerting repressive or no effects on the number of cytokine producers. Remarkably, in concentrations presumably found in lymphatic tissue in stress situations, noradrenaline suppressed lymphocyte proliferation completely. The shown repressive effects might especially have implications on health and welfare in pigs. The obtained results provide a preliminary database for extended studies on the molecular mechanisms of glucocorticoid and catecholamine actions on porcine immune cells.

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“…The results revealed no significant effects of any concentration of any hormone tested on any stage of embryo development ( Figure 1 ). Because it is known that the stress-induced serum concentrations of CRH [ 20 ], ACTH [ 21 ] and cortisol [ 22 ] in pigs are 1 × 10 −8 M, 3 × 10 −11 M and 4 × 10 −7 M, respectively, our results suggest that none of the three hormones impaired development of pig preimplantation embryos even at levels much higher than the physiological concentrations.…”

Section: Resultsmentioning

confidence: 80%

Hypothalamic-Pituitary-Adrenal Hormones Impair Pig Fertilization and Preimplantation Embryo Development via Inducing Oviductal Epithelial Apoptosis: An In Vitro Study

Wang

et al. 2022

Cells

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Previous studies show that stressful events after ovulation in sows significantly impaired the embryo cleavage with a significant elevation of blood cortisol. However, the effects of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and cortisol on fertilization and embryo development remain to be specified, and whether they damage pig embryos directly or indirectly is unclear. This study demonstrated that embryo development was unaffected when pig parthenotes were cultured with different concentrations of CRH/ACTH/cortisol. However, embryo development was significantly impaired when the embryos were cocultured with pig oviductal epithelial cells (OECs) in the presence of CRH/cortisol or cultured in medium that was conditioned with CRH/cortisol-pretreated OECs (CRH/cortisol-CM). Fertilization in CRH/cortisol-CM significantly increased the rates of polyspermy. CRH and cortisol induced apoptosis of OECs through FAS and TNFα signaling. The apoptotic OECs produced less growth factors but more FASL and TNFα, which induced apoptosis in embryos. Pig embryos were not sensitive to CRH because they expressed no CRH receptor but the CRH-binding protein, and they were tolerant to cortisol because they expressed more 11-beta hydroxysteroid dehydrogenase 2 (HSD11B2) than HSD11B1. When used at a stress-induced physiological concentration, while culture with either CRH or cortisol alone showed no effect, culture with both significantly increased apoptosis in OECs. In conclusion, CRH and cortisol impair pig fertilization and preimplantation embryo development indirectly by inducing OEC apoptosis via the activation of the FAS and TNFα systems. ACTH did not show any detrimental effect on pig embryos, nor OECs.

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Restraint effects on stress-related hormones and blood natural killer cell cytotoxicity in pigs with a mutated ryanodine receptor

Cited by 11 publications

References 45 publications

Succumbing to the laws of attraction

Succumbing to the laws of attraction

Glucocorticoids and Catecholamines Affect in Vitro Functionality of Porcine Blood Immune Cells

Hypothalamic-Pituitary-Adrenal Hormones Impair Pig Fertilization and Preimplantation Embryo Development via Inducing Oviductal Epithelial Apoptosis: An In Vitro Study

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