Restoration Of Glutamine Synthetase Activity, Nitric Oxide Levels And Amelioration Of Oxidative Stress By Propolis In Kainic Acid Mediated Excitotoxicity

Swamy, Mummedy; Norlina, Wan; Azman, Wan Norlina Wan; Suhaili, Dian; Sirajudeen, K. N. S.; Zulkarnain, Muhammad; Govindasamy, Chandran

doi:10.4314/ajtcam.v11i2.33

Cited by 19 publications

(16 citation statements)

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“…Other study on propolis, which is another beehive product, appeared to be effective in preventing KA-induced neuronal loss in the regions of the hippocampus [11]. In addition, propolis was found to attenuate KA-induced seizures and KA-induced oxidative stress in the brain thought to be due to its antioxidant property [11, 38]. These studies suggested that these antioxidants provide protection against KA-induced neuronal degeneration and seizures through antioxidant mechanism.…”

Section: Discussionmentioning

confidence: 99%

Effect of tualang honey against KA-induced oxidative stress and neurodegeneration in the cortex of rats

Sairazi

Sirajudeen

Asari

et al. 2017

BMC Complement Altern Med

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BackgroundAdministration of KA on rodents has resulted in seizures, behavioral changes, oxidative stress, and neuronal degeneration on selective population of neurons in the brain. The present study was undertaken to investigate the extent of neuroprotective effect conferred by Malaysian Tualang Honey (TH), an antioxidant agent, in the cerebral cortex of rats against KA-induced oxidative stress and neurodegeneration in an animal model of KA-induced excitotoxicity.MethodsMale Sprague–Dawley rats were randomly divided into five groups: Control, KA-treated group, TH + KA-treated group, aspirin (ASP; anti-inflammatory agent) + KA-treated group and topiramate (TPM; antiepileptic agent) + KA-treated group. The animals were pretreated orally with drinking water, TH (1.0g/kg BW), ASP (7.5mg/kg BW) or TPM (40mg/kg BW), respectively, five times at 12 h intervals. KA (15mg/kg BW) was injected subcutaneously 30 min after last treatment to all groups except the control group (normal saline). Behavioral change was observed using an open field test (OFT) to assess the locomotor activity of the animals. Animals were sacrificed after 2 h, 24 h and 48 h of KA administration.ResultsKA significantly inflicted more neuronal degeneration in the piriform cortex and heightened the predilection to seizures as compared with the control animals. Pretreatment with TH reduced the KA-induced neuronal degeneration in the piriform cortex but failed to prevent the occurrence of KA-induced seizures. In the OFT, KA-induced animals showed an increased in locomotor activity and hyperactivity and these were attenuated by TH pretreatment. Furthermore, TH pretreatment significantly attenuated an increase of thiobarbituric acid reactive substances level and a decrease of total antioxidant status level enhanced by KA in the cerebral cortex.ConclusionThese results suggest that pretreatment with TH has a therapeutic potential against KA-induced oxidative stress and neurodegeneration through its antioxidant effect.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1534-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Effect of tualang honey against KA-induced oxidative stress and neurodegeneration in the cortex of rats

Sairazi

Sirajudeen

Asari

et al. 2017

BMC Complement Altern Med

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“…ese beneficial effects of propolis could have been attributed to its bioactive compounds as well as the combined actions of these compounds [69]. Propolis has been reported to attenuate nitric oxide level, glutamine synthetase activity, oxidative stress, tumor necrosis factor-alpha (TNF-α), and caspase-3 activity, reduce seizures, and prevent neuronal loss in kainic acid-induced excitotoxicity rat model [74][75][76]. ese findings suggested that propolis exhibits its inhibitory effect on the oxidative stress, proinflammatory cytokines, and apoptosis as well as protection against neuronal damage through its anti-inflammatory, antioxidant, and antiapoptotic properties.…”

Section: Neuroprotective Activities Of Natural Products and Their Biomentioning

confidence: 99%

Natural Products and Their Bioactive Compounds: Neuroprotective Potentials against Neurodegenerative Diseases

Sairazi

Sirajudeen

2020

Evidence-Based Complementary and Alternative Medicine

130

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In recent years, natural products, which originate from plants, animals, and fungi, together with their bioactive compounds have been intensively explored and studied for their therapeutic potentials for various diseases such as cardiovascular, diabetes, hypertension, reproductive, cancer, and neurodegenerative diseases. Neurodegenerative diseases, including Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis are characterized by the progressive dysfunction and loss of neuronal structure and function that resulted in the neuronal cell death. Since the multifactorial pathological mechanisms are associated with neurodegeneration, targeting multiple mechanisms of actions and neuroprotection approach, which involves preventing cell death and restoring the function to damaged neurons, could be promising strategies for the prevention and therapeutic of neurodegenerative diseases. Natural products have emerged as potential neuroprotective agents for the treatment of neurodegenerative diseases. This review focused on the therapeutic potential of natural products and their bioactive compounds to exert a neuroprotective effect on the pathologies of neurodegenerative diseases.

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“…It was subjected to 80% ethanol extract as per the procedure of Isla et al (2001) as described by Swamy et al (2014). Single lot of propolis was used for extraction and used for administration to rats.…”

Section: Propolis Collection and Ethanol Extractionmentioning

confidence: 99%

Propolis Ameliorates Tumor Nerosis Factor-α, Nitric Oxide levels, Caspase-3 and Nitric Oxide Synthase Activities in Kainic Acid Mediated Excitotoxicity in Rat Brain

Swamy¹,

Suhaili²,

Sirajudeen³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Increased nitric oxide (NO), neuronal inflammation and apoptosis have been proposed to be involved in excitotoxicity plays a part in many neurodegenerative diseases. To understand the neuro-protective effects of propolis, activities of Nitric oxide synthase (NOS) and caspase-3 along with NO and tumor necrosis factor-α (TNF-α) levels were studied in cerebral cortex (CC), cerebellum (CB) and brain stem (BS) in rats supplemented with propolis prior to excitotoxic injury with kainic acid (KA). Materials and methods: Male Sprague-Dawley rats were divided into four groups (n=6 rats per group) as Control, KA, Propolis and KA+Propolis. The control group and KA group have received vehicle and saline. Propolis group and propolis + KA group were orally administered with propolis (150mg/kg body weight), five times every 12 hours. KA group and propolis +KA group were injected subcutaneously with kainic acid (15mg/kg body weight) and were sacrificed after 2 hrs. CC, CB and BS were separated, homogenized and used for estimation of NOS, caspase-3, NO and TNF-α by commercial kits. Results were analyzed by one way ANOVA, reported as mean + SD (n=6 rats), and p<0.05 was considered statistically significant. Results: The concentration of NO, TNF-α, NOS and caspase-3 activity were increased significantly (p<0.001) in all the three brain regions tested in KA group compared to the control. Propolis supplementation significantly (p<0.001) prevented the increase in NOS, NO, TNF-α and caspase-3 due to KA. Conclusion:Results of this study clearly demonstrated that the propolis supplementation attenuated the NOS, caspase-3 activities, NO, and TNF-α concentration and in KA mediated excitotoxicity. Hence propolis can be a possible potential protective agent against excitotoxicity and neurodegenerative disorders.

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Restoration Of Glutamine Synthetase Activity, Nitric Oxide Levels And Amelioration Of Oxidative Stress By Propolis In Kainic Acid Mediated Excitotoxicity

Cited by 19 publications

References 50 publications

Effect of tualang honey against KA-induced oxidative stress and neurodegeneration in the cortex of rats

Effect of tualang honey against KA-induced oxidative stress and neurodegeneration in the cortex of rats

Natural Products and Their Bioactive Compounds: Neuroprotective Potentials against Neurodegenerative Diseases

Propolis Ameliorates Tumor Nerosis Factor-α, Nitric Oxide levels, Caspase-3 and Nitric Oxide Synthase Activities in Kainic Acid Mediated Excitotoxicity in Rat Brain

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