2008
DOI: 10.1590/s1984-29612008000200003 View full text |Buy / Rent full text
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Abstract: O objetivo do presente estudo foi analisar a resposta imune humoral anti-Anaplasma marginale em búfalos (Bubalus bubalis) naturalmente infectados. Amostras de soro e de colostro/leite coletadas de búfalas adultas por um período de 335 dias após o parto e de soros dos seus bezerros do nascimento até 365 dias de vida, foram usadas para a realização do método ELISA indireto. Os dados foram analisados como a média de um grupo de animais, em diferentes faixas etárias, durantes os anos de 1999/2000 e individ… Show more

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“…Positive controls were from two adult cattle experimentally infected with the "Lins" isolate of T. vivax (Fidelis et al, 2016). The mean and standard deviation (SD) of the optical densities (OD) obtained from the negative controls of all plates were calculated, and from these results the cutoff was established according to the following equation, described by Gomes et al (2008): Cutoff = Mean of negative controls x 2.5…”
Section: Diagnostic Methodsmentioning
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“…Positive controls were from two adult cattle experimentally infected with the "Lins" isolate of T. vivax (Fidelis et al, 2016). The mean and standard deviation (SD) of the optical densities (OD) obtained from the negative controls of all plates were calculated, and from these results the cutoff was established according to the following equation, described by Gomes et al (2008): Cutoff = Mean of negative controls x 2.5…”
Section: Diagnostic Methodsmentioning
“…O carrapato Rhipicephalus microplus (Starke et al 1994) e as moscas Haematobia irritans (Allingham et al 1994) e Stomoxys calcitrans (Potgieter et al 1981) podem efetuar o papel importante na transmissão deste patógeno para os búfalos (Mohan 1968, Costa et al 1997, Gomes et al 2008.…”
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