Responsive photonic encoded breathing microbeads based microfluidic chip for multiplex fluorescent immunoassay

Luan, Chengxin; Xu, Yueshuang; Chen, Baoan; Yang, Zixue

doi:10.1016/j.snb.2016.09.065

Cited by 13 publications

(5 citation statements)

References 18 publications

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“…LODs in pM were 35 for IL-6 (0.78 mg/mL), 25.0 (0.30 ng/mL) for IL-8, and 30.8 for MCP-1 (0.38 ng/mL) at 50 °C. Another suspension array was reported by Luan et al, who used plasmonic-encoded reverse opal microbeads integrated into a special microfluidic chip . These so-called solvent-responsive breathing microbeads were used in a specially designed capillary microfluidic chip to give better performance than silica colloidal crystal bead arrays.…”

Section: Magnetic Bead- and Microsphere-assisted Assaysmentioning

confidence: 99%

“…Another suspension array was reported by Luan et al, who used plasmonic-encoded reverse opal microbeads integrated into a special microfluidic chip. 75 These so-called solvent-responsive breathing microbeads were used in a specially designed capillary microfluidic chip to give better performance than silica colloidal crystal bead arrays. The authors claimed an order of magnitude improvement in sensitivity for photonic encoded microbead arrays, but the method was used only to detect a single analyte, the protein AFP with 60 pg/mL LOD.…”

Section: Analytical Chemistrymentioning

confidence: 99%

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Multiplexed Immunosensors and Immunoarrays

et al. 2019

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Immunoassays make use of highly specific antigen-antibody binding and provide sensitive ways to detect a wide range of biomacromolecules, bacteria, viruses, and small molecules. There are a range of types of immunoassay systems including single analyte sensors, 96well plate formats, arrays, microfluidic sensors, microfluidic arrays, etc. A big target is encompassed by medical diagnostic biomarkers, which are "molecules that can be measured objectively as indicators of normal or disease processes and responses to therapeutic intervention". 1 Accurate, low-cost measurements of multiple proteins are major applications of immunoarrays that are critical for future early detection and monitoring of cancer and other diseases. Multiplexing is very important, since panels of biomarker proteins, as opposed to single biomarkers, are required to provide sufficient information content for reliable disease diagnostics.

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Section: Magnetic Bead- and Microsphere-assisted Assaysmentioning

confidence: 99%

Section: Analytical Chemistrymentioning

confidence: 99%

Multiplexed Immunosensors and Immunoarrays

et al. 2019

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“…Numerous other 3D optical crystal devices have been fabricated for diverse sensing applications. Notably, Luan et al, 2017 developed silica colloidal crystal microbeads with molecular imprinting for multiplex fluorescent immunoassay [ 34 ]. Lu et al, 2017 developed a colorimetric sensor arrays for nitroaromatic compounds detection [ 35 ].…”

Section: Sensing Applicationsmentioning

confidence: 99%

Stimuli Responsive Polymer-Based 3D Optical Crystals for Sensing

2017

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3D optical crystals have found their applications in sensing, actuation, optical devices, batteries, supercapacitors, etc. The 3D optical crystal devices are comprised of two main components: colloidal gels and nanoparticles. Nanoparticles self-assemble into face center cubic structures in colloidal gels. The inherent 3D optical crystal structure leads to display of structural colors on these devices following light impingement. As such, these optical properties have led to the utilization of these 3D optical crystals as self-reporting colorimetric sensors, which is the focus of this review paper. While there is extensive work done so far on these materials to exhaustively be covered in this review, we focus here in on: mechanism of color display, materials and preparation of 3D optical crystals, introduction of recent sensing examples, and combination of 3D optical crystals with molecular imprinting technology. The aim of this review is to familiarize the reader with recent developments in the area and to encourage further research in this field to overcome some of its challenges as well as to inspire creative innovations of these materials.

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“…So far, CEA detection methods include radioimmunoassay, enzyme-linked immunoassay, time-resolved fluorescence, chemiluminescence, flow injection system based immunosensors, etc [5][6][7][8][9]. However, these methods have their own limits in preparation such as being unfriendly to the environment, time-consuming, difficult to achieve automation, or have poor accuracy [3,5].…”

Section: Introductionmentioning

confidence: 99%

Microstructure evolution of sandwich graphite oxide/interlayer-embedded Au nanoparticles induced from γ-rays for carcinoembryonic antigen biosensor

et al. 2019

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With the capability of inducing small particle sizes of supported metal in graphite oxide (GO), the γ-ray irradiation method applied for preparing graphite oxide-gold (GO-Au) nanocomposites as electrochemical immunosensors has attracted specific attention recently. To study the accurate factors influencing the precise morphology and final performance of the prepared composites in the γ-irradiation system, we proposed a facile method to investigate the evolution of the GO structure, size and dispersion of Au nanoparticles (AuNPs) produced with the addition of isopropyl alcohol to the system. The GO-Au nanocomposites were characterized by Fourier transform infrared spectroscopy, x-ray diffraction spectra, Raman spectra, x-ray photoelectron spectroscopy and high resolution transmission electron microscopy. These nanocomposites with sandwich morphology exhibited an excellent immunosensor performance with a low detection limit of 15.8 pg ml −1 (S/N=3) and a wide linear range from 1 to 40 ng ml −1 for detecting carcinoembryonic antigens. The enhanced biosensing performance is attributed to the synergistic effect of γ-irradiation and the precise structure of GO, which endows the smaller size and more uniform distribution of AuNPs on the GO as well as the good signal amplification capability. Furthermore, adopting the γ-irradiation method and use of GO as a precursor is propitious for application in large-scale production because of its high-efficiency and high-yielding characteristics.

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Responsive photonic encoded breathing microbeads based microfluidic chip for multiplex fluorescent immunoassay

Cited by 13 publications

References 18 publications

Multiplexed Immunosensors and Immunoarrays

Multiplexed Immunosensors and Immunoarrays

Stimuli Responsive Polymer-Based 3D Optical Crystals for Sensing

Microstructure evolution of sandwich graphite oxide/interlayer-embedded Au nanoparticles induced from γ-rays for carcinoembryonic antigen biosensor

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