Responses of Sri Lankan indigenous goats and their Jamnapari crosses to artificial challenge with Haemonchus contortus

Kurukulasuriya, Maheshika S.; Silva, Pradeepa; Dematawewa, C.M.B.; Ariyaratne, H.; Rajapakshe, Rgas; Wickramaratne, S.H.G.; Jayasooriya, L. J. P. A. P.; Munasinghe, D.M.S.; Lokugalappatti, L. G. S.; Notter, D. R.

doi:10.1016/j.vprsr.2017.11.011

Cited by 3 publications

(8 citation statements)

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“…Additionally, the challenge trial also showed that resistant YWG had a tendency to delayed egg production and shed fewer eggs at day 28, 35, and 42 of post-infection. A similar result was also reported by several scientists on artificial challenge trial with infective L 3 larvae of H. contortus between resistant and susceptible goats (González et al 2008;Kurukulasuriya et al 2018). The phenotypic of the resistance group in the term of FEC indicates an anti-fecundity effect of the immune response against parasitic infection.…”

Section: Discussionsupporting

confidence: 83%

“…Our selective immune genes were differentially expressed when goats were experimentally challenged with infective L 3 larvae of H. contortus in resistant and susceptible goats. The FEC is generally considered to be the main indicator of H. contortus infection in sheep and goats (Rodríguez et al 2015;Omar et al 2016;Kurukulasuriya et al 2018). Previous reports revealed that resistant goats shed lower FEC and rapid recovery from initial elevations in FEC (Pralomkarn et al 1997;Makun et al 2008;Chiejina et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Eight goats (4 resistant and 4 susceptible ) were selected to dry lot for challenge trial and treated with broad-spectrum anthelmintic viz., Ivermectin (0.25 mg/kg body weight) and 15 days later, with levamisole (8 mg/kg body weight) to ensure the animals were completely dewormed (Bambou et al 2013; Kurukulasuriya et al 2018). In dry lot, each goat were fed 30 g/kg BW of balance feed including GINfree fresh grass per day on a dry-matter basis, and a concentrated mixture was provided twice per day at a rate of 1.5% of BW containing 18% crude protein and 10.6UJ/kg of metabolizable energy on a dry matter basis (Ojeda-Robertos et al 2017).…”

Section: Nematode Challenge Trialmentioning

confidence: 99%

“…In dry lot, each goat were fed 30 g/kg BW of balance feed including GINfree fresh grass per day on a dry-matter basis, and a concentrated mixture was provided twice per day at a rate of 1.5% of BW containing 18% crude protein and 10.6UJ/kg of metabolizable energy on a dry matter basis (Ojeda-Robertos et al 2017). When all experimental goats achieved a FEC of zero ensured by microscopic examination, resistant and susceptible goats were inoculated with a single dose of 5,000 thirdstage (L3) larvae of H. contortus (Notter et al 2003;Getachew et al 2015;Kurukulasuriya et al 2018). The day of inoculation was considered to be day 0, and FEC, PCV, Hg, and BW were determined weekly for six weeks from the day of inoculation.…”

Section: Nematode Challenge Trialmentioning

confidence: 99%

“…A number of studies described the genetic resistance to GIN of different sheep breeds, and it is mainly associated with the immune responses of hosts. However, few studies have been conducted on the immune response of goats to H. contortus infection (Baker et al 2001;Chiejina & Behnke 2011;Omar et al 2019;Kurukulasuriya et al 2018). Several goats and sheep breeds are naturally resistant to gastrointestinal parasites infection and evaluating the relative resistance of goat breeds using artificial challenge trial should be considered as a way towards mapping of genes controlling internal parasite (Getachew et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

et al. 2019

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Our previous association study revealed the mutation in candidate immune genes (NOD1 & NLRP9) was significantly associated with FEC of Haemonchus contortus infection in Yichang white goats, but the relative expression of mRNA of those genes associated with resistance to H. contortus was not investigated. Aim of the current experiment was to evaluate the susceptible and resistant individuals to nematode infection within the population of Yichang white goat (YWG) and assess the differential level of mRNA expression of those candidate genes in the abomasal tissues of susceptible and resistant goats. Fecal egg count (FEC) was determined using a modified McMaster technique, and the hematological parameter was measured by Mindray auto hematology analyzer. Phenotype data were collected and analyzed using a generalized linear model with SAS statistical program. Field investigate revealed that the prevalence (76%) with maxium parasite load (734.34±84.21epg) of H. contortus occurred in August within the experimental flock FEC in resistant group (103.38±1.20epg) and susceptible group (1180.25±43.53epg) group demonstrated the presence of two distinct goat populations within this breed. Four resistant and four susceptible goats were selected from each group. The parasite infection was established by artificially challenge with 5000 infective L3 larvae of H. contortus. Abomasal tissues were collected from all experimental goats after 42 days of post-infection. FEC, Body weight, packed cell volume, and hemoglobin value were significantly different (P < 0.01) between resistant and susceptible group of goats. Quantitative real-time PCR in abomasal tissues revealed that the expression level of mRNA for NOD1 (P < 0.00001), IFNG (P < 0.0001), NLRP9, TLR8, IL32, (P < 0.001) and IGF1 (P < 0.01) was higher in resistant goat compared to susceptible, except SFTPA1. These findings revealed the presence of genetic resistant individuals to H. contortus within the goat breed and expression of NOD1 and NLRP9 genes proved the positive finding of our previous study. Presence of genetic resistant individuals in Yichang white goat YWG breed could be a good candidate for selective breeding and highly expressed genes related to resistant could be used as biomarkers to develop H. contortus resistant goat population. Progressive Agriculture 30 (2): 194-208, 2019

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Nematode Challenge Trialmentioning

confidence: 99%

Section: Nematode Challenge Trialmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

et al. 2019

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Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Alam

Omar

Faruque

et al. 2019

J Animal Sci Biotechnol

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BackgroundHaemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, Haemonchus contortus, which is highly pathogenic in small ruminants. The aim of this study was to identifying single-nucleotide polymorphisms (SNP) that were associated with fecal egg counts (FEC) and could be used as markers to identify resistance to H. contortus in goats.ResultsTen novel variants in the CIITA, ATP2A3, HSPA8, STAT5B, ESYT1, and SERPING1 genes were associated with FEC in goats with a nominal significance level of P < 0.05. Two missense mutation in the exon region of the caprine CIITA gene resulted in replacement of arginine with cysteine at position 9473550 (R9473550C) and aspartic acid with glutamic acid at position 9473870 (D9473870E). Chinese goat breeds had significantly higher FEC than Bangladeshi goat breeds within their respective genotypes. Polymorphism information content (PIC), effective allele number (Ne), and heterozygosity (He) were greatest for the STAT5B_197_A > G SNP locus in all goat breeds. Pairwise coefficients of linkage disequilibrium (D´, r2) revealed complete LD (r2 = 1) between significant SNP polymorphisms in CIITA and SERPING1 and strong LD (r2 = 0.93 and 0.98) between polymorphisms in HSPA8 and ATP2A3, respectively. Correlation coefficient (r) between FEC and body weight (BW) was significantly positive (r = 0.56***, P < 0.001) but that between FEC and packed cell volume (PCV) was negatively significant (r = − 0.47**, P < 0.01) in the total population of goats. On the other hand, correlation coefficient (r) between BW and PCV was not significant in total population of goats. Association analysis revealed that haplotypes within ATP2A3, HSPA8, and SERPING1 were significantly associated with FEC. Quantitative real-time PCR revealed that the relative expression of mRNA was higher (P < 0.001) for resistant, compared to susceptible, groups of goats for all candidate genes except CIITA.ConclusionsThis study identified SNP markers that can potentially be used in marker-assisted selection programs to develop goat breeds that are resistant to H. contortus.Electronic supplementary materialThe online version of this article (10.1186/s40104-019-0327-8) contains supplementary material, which is available to authorized users.

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Parásitos gastroentéricos, población haemonchus contortus en caprinos en clima semiárido de Bacum, Sonora, México

2018

Abanico Vet

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The gastrointestinal parasites (PGE), and in special Haemonchus contortus (HC) have been adapted to different ecosystems and cause direct and indirect damage to the goat production. In order to determine the frequency of PGE and parasitic burdens of HC in abomasum in naturally infected goats and sacrificed in a local slaughter in Bacum during 2015 to 2017, 1,823 feces samples were collected of adult goats. 815 (42.4 %) samples were positive to Strongylidea; 69 (3.69 %) were positive to Moniezia expansa and 5 (0.21 %) were positive to Skjabinema caprae. In the same period, 770 abomasums were collected and 447 (58.05 %) were positive to the presence of adult Haemonchus contortus. The parasitic burden of HC was 50,296: 33,222 (66.05 %) female and 17,074 (33.94 %) male. The parasite effects and its biotic potential of HC have been reviewed which represent the value of adaptation to different environments that allows the infection of new guests. It is concluded that the presence of PGE and the population of Haemonchus contortus in abomasum represent an abiotic potential essential for adaptation and survival in different environments. Biotic, Sonora, semiarid, abomasum, goat. RESUMEN Los parásitos gastroentericos (PGE), y en especial Haemonchus contortus (HC), se han logrado adaptar a diferentes ecosistemas y ocasionan daños directos e indirectos a la producción de caprinos. Con el objetivo de determinar la frecuencia de PGE y las cargas parasitarias en abomaso de HC en caprinos infectados en forma natural y sacrificados en un rastro local de Bacum. De 2015 al 2017 se recolectaron 1823 muestras de heces y 770 abomasos. Las heces de cabras adultas fueron positivas 815 (42.4%) a Strongylideos; 69 positivas (3.69%) a Moniezia expansa y 5 positivas (0.21%) a Skjabinema caprae. En la revisión de abomasos en tres años de estudio se encontró la presencia de Strongylideos spp y de Haemonchus contortus, se recolectaron 770 abomasos con 447 positivos (58.05%), la población total de HC fue 50,296: Hembras 33,222 (66.05%) y machos 17,074 (33.94%).Se revisa los efectos de los parásitos y el potencial biótico de HC que representa el valor en situación ideal de adaptación a diferentes ambientes que permite la infección de nuevos huéspedes.Se concluye que la presencia de PGE y población de Haemonchus contortus en abomaso representa un potencial biótico esencial de adaptación y sobrevivencia en diferentes ambientes. Biótico, Sonora, semiárido, abomaso, cabras.

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Responses of Sri Lankan indigenous goats and their Jamnapari crosses to artificial challenge with Haemonchus contortus

Cited by 3 publications

References 40 publications

Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Parásitos gastroentéricos, población haemonchus contortus en caprinos en clima semiárido de Bacum, Sonora, México

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