Response to metals treatment of Fra1, a member of the AP-1 transcription factor family, in P. lividus sea urchin embryos

Russo, Roberta; Bonaventura, Rosa; Chiaramonte, Marco; Costa, Caterina; Matranga, Valeria; Zito, Francesca

doi:10.1016/j.marenvres.2018.05.003

Cited by 13 publications

(12 citation statements)

References 68 publications

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“…To preliminarily explore the functions of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 with regard to the stress response, partial 5′-flanking regions of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 were chosen to predict possible cis -acting elements using TFBIND. We found many putative binding sites bound by stress response-related transcription factors, such as p53, NF-κB, CREB, and AP-1 ( Wan et al, 2012 ; Bagul et al, 2015 ; Charni-Natan et al, 2018 ; Chen et al, 2018 ; Fujimura and Usuki, 2018 ; Russo et al, 2018 ), in the 5′-flanking regions of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 , and a partial list of them is shown in Table 1 .…”

Section: Resultsmentioning

confidence: 99%

“…The activation of the p38 MAPK-CREB signaling pathway under MeHg-induced oxidative stress is conducive to neuronal cell death and cerebrocortical neuronal hyperactivity ( Fujimura and Usuki, 2018 ). Fra1, a member of the transcription factor family of AP-1, is associated with metal treatment response in Paracentrotus lividus embryos ( Russo et al, 2018 ). In HepG2 cells, p53 can change the different secretome of the liver and can influence whole organism homeostasis and liver function under various stress stimuli ( Charni-Natan et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

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Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Zhao

Zhang

et al. 2018

Front. Genet.

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DnaJ, also known as Hsp40, plays important roles in maintaining the normal physiological state of an organism under stress conditions by mediating essential processes, such as protein synthesis, degradation, folding and metabolism. However, the exact functions of most DnaJ members are not fully understood in insects. Here, we identified three genes, AccDnaJA1, AccDnaJB12, and AccDnaJC8, in Apis cerana cerana and explored their connection with the environmental stress response. Quantitative real-time PCR results showed that the mRNA levels of AccDnaJA1, AccDnaJB12, and AccDnaJC8 were all induced under cold, UV, H2O2 and different pesticides treatment. The expression patterns of AccDnaJB12 and AccDnaJC8 were upregulated by CdCl2 and HgCl2 stress, while the transcriptional levels of AccDnaJA1 were downregulated by CdCl2 and HgCl2 stress. Western blot findings further indicated that AccDnaJB12 protein levels were increased by some stress conditions. Knockdown of each of these three genes downregulated the transcriptional patterns of several stress response-related genes at different levels. Functional analysis further demonstrated that the resistance of A. cerana cerana to lambda-cyhalothrin stress was reduced with knockdown of AccDnaJA1, AccDnaJB12, or AccDnaJC8, indicating that these three genes may be involved in the tolerance to this pesticide. Taken together, these findings indicate that AccDnaJA1, AccDnaJB12, and AccDnaJC8 may play pivotal roles in the stress response by facilitating honeybee survival under some adverse circumstances. To our knowledge, this is the first report that reveals the roles of DnaJ family proteins under different adverse circumstances in A. cerana cerana.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Zhao

Zhang

et al. 2018

Front. Genet.

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“…In the last years, several studies were performed on the sea urchin innate immune system that reinforced the notion of its use as a model for immunological purpose, due to its evolutionary proximity to chordates and vertebrates (Branco et al., ; Rast, Smith, Loza‐Coll, Hibino, & Litman, ). Moreover, P. lividus was extensively used as a biological indicator of local pollution and of stress (Bonaventura, Zito, Chiaramonte, Costa, & Russo, ; Russo et al., ; Scanu et al., ).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary conserved pathway of the innate immune response after a viral insult in Paracentrotus lividus sea urchin

Chiaramonte

Arizza

Russo

2019

Int J Immunogenetics

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Despite the apparent simplicity of the body organization of echinoderms, their immune system is competent to perform a complex innate immune response, which is far from being well understood. The echinoderms represent the most advanced invertebrates that form a bridge with the primitive chordates. In fact, they possess numerous receptors and effectors that are used to obtain a fast immune response. After an infection, the humoral and cellular immune response determines a network in which the main protagonists are membrane and endosomal receptors. The recognition of nonself molecules by specific membrane receptors triggers the immune response, stimulating consecutive intracellular events. We have previously shown how the polyinosinic–polycytidylic acid (polyI:C) that mimics a viral infection is able to induce an immune response in the sea urchin Paracentrotus lividus immune cells. It activates a specific membrane receptor belonging to the Toll‐like receptor (TLR) family. Here, we show the activated expression pattern of some genes involved in the downstream cascade of TLR signalling pathway, such as Pl‐Tbk and Pl‐Irf, whose partial sequence was isolated from P. lividus immune cells. Their mRNA expression increases consequentially to the polyI:C stimulation and in a temporal way. In addition, we analysed the expression of Pl‐NF‐kB and we found that its upregulation was time‐dependent, preceding Pl‐Tbk and Pl‐Irf increase. Protein analysis showed that also some cytokine (TNF‐α and IL‐1α) expression increased after polyI:C insult. Therefore, the purpose of this study was to discover the molecular mechanisms of the innate defence strategies, similarly to vertebrates, implemented by the sea urchins in order to cope with viral infection challenge.

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“…To study the embryonic response induced by Li, Ni, and Zn in P. lividus sea urchin embryos, we took advantage of a previous study [26]. Briefly, embryos were continuously cultured in the presence of 30 mM LiCl (Li) (Sigma-Aldrich, St. Louis, MO, USA), 0.5 mM NiCl 2 (Ni) (Sigma-Aldrich, St. Louis, MO, USA) and 0.1 mM ZnSO 4 (Zn) (Sigma-Aldrich, St. Louis, MO, USA), henceforth defined as Li-embryos, Ni-embryos, and Zn-embryos, respectively.…”

Section: Metals Treatment Of P Lividus Embryosmentioning

confidence: 99%

“…Total RNA from P. lividus control and treated embryos, the same utilized for the nCounter Nanostring analysis, was reverse transcribed to obtain the corresponding cDNA as described in Russo et al [26]. In order to quantify gene expression, the cDNAs were amplified by using SYBR Green technology, based on a Comparative Threshold Cycle Method [29], according to the manufacturer's instructions (Applied Biosystems StepOne-Plus instrument, Grand Island, NY, USA) and as previously described [26]. Pl-Z12-1 mRNA was used as a reference gene.…”

Section: Real-time Quantitative Pcr (Qpcr)mentioning

confidence: 99%

Gene Expression Analysis of the Stress Response to Lithium, Nickel, and Zinc in Paracentrotus lividus Embryos

Bonaventura

Costa

Deidda

et al. 2022

Toxics

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Many anthropogenic pollutants such as metals are discharged into the marine environment through modern sources. Among these, lithium (Li), nickel (Ni), and zinc (Zn) can interfere with biological processes in many organisms when their concentration rises. These metals are toxic to sea urchin embryos, affecting their development. Indeed, animal/vegetal and dorso/ventral embryonic axes are differently perturbed: Li is a vegetalizing agent, Ni can disrupt dorso-ventral axis, Zn can be animalizing. To address the molecular response adopted by embryos to cope with these metals or involved in the gene networks regulating embryogenesis, and to detect new biomarkers for evaluating hazards in polluted environments in a well-known in vivo model, we applied a high-throughput screening approach to sea urchin embryos. After fertilization, Paracentrotus lividus embryos were exposed to Li, Ni, and Zn for 24/48 h. At both endpoints, RNAs were analyzed by NanoString nCounter technology. By in silico analyses, we selected a panel of 127 transcripts encoding for regulatory and structural proteins, ranked in categories: Apoptosis, Defense, Immune, Nervous, Development, and Biomineralization. The data analysis highlighted the dysregulation of many genes in a metal-dependent manner. A functional annotation analysis was performed by the KEEG Orthology database. This study provides a platform for research on metals biomarkers in sea urchins.

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Response to metals treatment of Fra1, a member of the AP-1 transcription factor family, in P. lividus sea urchin embryos

Cited by 13 publications

References 68 publications

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Evolutionary conserved pathway of the innate immune response after a viral insult in Paracentrotus lividus sea urchin

Gene Expression Analysis of the Stress Response to Lithium, Nickel, and Zinc in Paracentrotus lividus Embryos

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