Response surface analysis of nano-ureases from Canavalia ensiformis and Cajanus cajan

Dwevedi, Alka; Routh, Satya Brata; Yadav, Amit Singh; Singh, Ashwani Kumar; Srivastava, O. N.; Kayastha, Arvind M.

doi:10.1016/j.ijbiomac.2011.06.027

Cited by 9 publications

(4 citation statements)

References 34 publications

(55 reference statements)

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“…Nanotechnology has immense potential to produce major advances in areas of enzyme immobilization, energy, food, agriculture and medicine 42. Nanotechnology-based delivery systems can overcome shortcomings such as drug solubility, permeability and early clearance issues associated with small molecules and drugs, and can provide smart therapeutic solutions such as tissue-specific chemotherapy and phototherapy 43–46…”

Section: Discussionmentioning

confidence: 99%

<p>Functional design of pH-responsive folate-targeted polymer-coated gold nanoparticles for drug delivery and in vivo therapy in breast cancer</p>

Mahalunkar¹,

Yadav²,

Gorain³

et al. 2019

IJN

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BackgroundCurcumin has been widely used owing to its various medicinal properties including antitumor effects. However, its clinical application is limited by its instability, poor solubility and low bioavailability. Folic acid (FA)-functionalized nanoformulations may enhance the sustained release of an anticancer drug (curcumin) by tumor-specific targeting to improve therapeutic benefit. This study aims to design a nanoconjugate (NC) comprised of folate–curcumin-loaded gold–polyvinylpyrrolidone nanoparticles (FA–CurAu-PVP NPs) for targeted delivery in breast cancer model systems.MethodsWe developed curcumin-loaded FA-functionalized Au-PVP NCs by layer-by-layer assembly. The folic acid–curcumin Au-PVP NCs (FA–CurAu-PVP NCs) were characterized by ultraviolet–visible spectra, Fourier transform infrared spectroscopy, X-ray powder diffraction and thermogravimetric analysis. In vitro anticancer and antimigratory effects of NCs were examined by performing MTT and wound migration assays. The in vivo antitumor efficacy of NCs was investigated using a preclinical breast cancer orthotopic mouse model.ResultsCurcumin (40 µg/mL) was loaded along with conjugation of folate onto Au-PVP NPs to form FA–CurAu-PVP NCs. The size and charge of the NCs were increased gradually through layer-by-layer assembly and showed 80% release of curcumin at acidic pH. The NC did not show aggregation when incubated with human serum and mimicked an intrinsic peroxidase-like property in the presence of 3,3ʹ,5,5ʹ-tetramethylbenzidine substrate. The MTT data using these NCs showed efficient anticancer activity at lower doses in estrogen/progesterone receptor (ER/PR)-negative cells compared with ER/PR-positive cells. Furthermore, the NCs did not show cytotoxicity at the investigated concentration in human breast epithelial and mouse fibroblast cell lines. They showed inhibitory effects on cell migration and high antitumor efficacy in in vivo analysis.ConclusionThese results suggest that folate-based tumor targeting using CurAu-PVP NCs is a promising approach for tumor-specific therapy of breast cancer without harming normal cells.

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Section: Discussionmentioning

confidence: 99%

<p>Functional design of pH-responsive folate-targeted polymer-coated gold nanoparticles for drug delivery and in vivo therapy in breast cancer</p>

Mahalunkar¹,

Yadav²,

Gorain³

et al. 2019

IJN

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“…Jack bean and pigeonpea ureases are the most studied plant ureases . Pigeonpea urease was purified, characterized, immobilized, and a prototype urea biosensor was built. − Urease from soybeans provides the best plant genetic information. , Plant and fungal ureases are homo-oligomeric proteins with identical subunits, unlike bacterial ones . Plant and bacterial urease share a catalytic mechanism, sequence similarity, and 3-D structure .…”

Section: Introductionmentioning

confidence: 99%

“… 7 Pigeonpea urease was purified, characterized, immobilized, and a prototype urea biosensor was built. 8 − 13 Urease from soybeans provides the best plant genetic information. 14 , 15 Plant and fungal ureases are homo-oligomeric proteins with identical subunits, unlike bacterial ones.…”

Section: Introductionmentioning

confidence: 99%

In Silico Structural and Functional Insight into the Binding Interactions of the Modeled Structure of Watermelon Urease with Urea

Kumar,

Kayastha,

Singh

et al. 2023

ACS Omega

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Urease (EC 3.5.1.5) is an amidohydrolase. This nickel-dependent metalloenzyme converts urea into NH 3 and CO 2 . Despite their vital role in plants, the structure and function of watermelon (Citrullus lanatus) urease are unknown. We used third-and fourth-generation gene prediction algorithms to annotate the C. lanatus urease sequence in this investigation. The solved urease structure from Canavalia ensiformis (PDB ID: 4GY7) was utilized as a template model to identify the target 3-D model structure of the unknown C. lanatus urease for the first time. Cluretox, the C. lanatus urease intrinsic disordered area identical to Jaburetox, was also found. The C. lanatus urease structure was docked with urea to study atom interaction, amino acid interactions, and binding analyses in the urease−urea complex at 3.5 Å. This study found that amino acids His 517 ,

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“…For these applications, neutral urease obtained from seeds of plant bean species such as canavalia ensiformis, or from microorganisms such as eubacterium aerofaciens and P. mirabilis are very useful and yielding good results. 4 There is ongoing approach to explore cheap sources of urease, which are readily available and have better physico-chemical properties.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Urease on DEAE-Cellulose Strips for One Step Urea Detection

Kayastha

2019

Annals of the National Academy of Medical Sciences (India)

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Urease isolated from pigeonpeas was immobilized on 1 cm × 6 cm diethylaminoethyl (DEAE)-cellulose strips. The optimum immobilization (55% activity) was observed at 4°C, with a protein concentration of 1 mg per strip at pH 6.5. Immobilized strips stored at 4°C showed an increased shelf-life and there was no leaching of enzyme from the immobilized strips for 6 months. Enzyme could be eluted on application of 0.2 M KCl in buffer, which showed that the binding of the enzyme was tight on DEAE-cellulose strips. These strips when used along with dye, phenol red (coimmobilized) gave different shades from light yellow to dark magenta depending upon the concentration of urea. The ease of immobilization of urease on DEAE-cellulose strips and easy availability of pigeonpeas urease described in the present study makes it a suitable product for potential applications in urea diagnostics.

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Response surface analysis of nano-ureases from Canavalia ensiformis and Cajanus cajan

Cited by 9 publications

References 34 publications

<p>Functional design of pH-responsive folate-targeted polymer-coated gold nanoparticles for drug delivery and in vivo therapy in breast cancer</p>

<p>Functional design of pH-responsive folate-targeted polymer-coated gold nanoparticles for drug delivery and in vivo therapy in breast cancer</p>

In Silico Structural and Functional Insight into the Binding Interactions of the Modeled Structure of Watermelon Urease with Urea

Immobilization of Urease on DEAE-Cellulose Strips for One Step Urea Detection

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