Response of the soil bacterial community to the addition of toluene and toluene-degrading bacteria

Chao, Wei-Liang; Hsu, Shi-Fang

doi:10.1016/j.soilbio.2003.10.025

Cited by 16 publications

(7 citation statements)

References 41 publications

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“…The numerical dominance of bacterial members belonging to the γ-Proteobacteria, mainly species of Pseudomonas and Acinetobacter, in this study appeared to further corroborate previous documentations that have also revealed high occurrences or community shift towards these particular bacterial phylotype, among indigenous hydrocarbondegrading microbial communities in response to hydrocarbon contamination [16,27,28]. Specifically, members of the pseudomonads observed to be dominant in the three microcosms examined in this study have also been shown to be prolific hydrocarbon degraders in several studies of contaminated environments [9,15,[28][29][30].…”

Section: Gamma Diversity 11supporting

confidence: 89%

“…Specifically, members of the pseudomonads observed to be dominant in the three microcosms examined in this study have also been shown to be prolific hydrocarbon degraders in several studies of contaminated environments [9,15,[28][29][30]. Probably because the pseudomonads are copiotrophic with propensity for high concentrations of nutrients [31], thereby giving them the edge in typically outnumbering other bacterial competitors within any contaminated environment.…”

Section: Gamma Diversity 11mentioning

confidence: 81%

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Phylogenetic Characterization and Community Diversity of Hydrocarbon-Utilizing Bacteria in Soil Microcosms Enriched with Aromatic Hydrocarbons

A¹

2015

J Bioremed Biodeg

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Section: Gamma Diversity 11supporting

confidence: 89%

Section: Gamma Diversity 11mentioning

confidence: 81%

Phylogenetic Characterization and Community Diversity of Hydrocarbon-Utilizing Bacteria in Soil Microcosms Enriched with Aromatic Hydrocarbons

A¹

2015

J Bioremed Biodeg

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“…The ratio between Proteobacteria and the Acidobacterium division might also increase with the trophic level of soils (Smit et al, 2001), a tendency which is also visible when bacterial communities of hydrocarbon-contaminated and corresponding uncontaminated soils are compared (Saul et al, 2005). A c-shift has also been reported to occur upon contamination of soil (Chao & Hsu, 2004) and Arctic sea ice (Gerdes et al, 2005). In the present case (Rositz), the contamination with 8000 milligrams mineral oil hydrocarbons per kilogram soil dry weight provided a high nutrient supply for degraders.…”

Section: Predominance Of Gammaproteobacteriamentioning

confidence: 93%

“…A high prevalence of Acinetobacter has been reported mostly in connection with diesel contamination. Thus, the Acinetobacter concentration was reported to increase in soil within a few days of contamination with diesel (Chao & Hsu, 2004). Acinetobacter were also the most abundant diesel-degrading isolates during a bioremediation test with diesel-contaminated sandy soil (Gallego et al, 2001), which was biostimulated with nutrients and periodically tilled for aeration.…”

Section: Predominance Of Gammaproteobacteriamentioning

confidence: 99%

“…Various methods have been used to analyse the microbial diversity in mineral oil hydrocarbon-contaminated soils. One method is the cultivation of bacteria (Chao & Hsu, 2004;Greene et al, 2000;La Rosa et al, 2006;Plaza et al, 2003;Radwan et al, 1995;Saul et al, 2005), but it has been estimated that only~1 % of soil micro-organisms can be cultivated by conventional methods (Torsvik et al, 1990). The established cultivation methods favour the isolation of fast-growing microorganisms, and those that accept high nutrient concentrations (Felske et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial diversity in the active stage of a bioremediation system for mineral oil hydrocarbon-contaminated soils

2006

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Soils contaminated with mineral oil hydrocarbons are often cleaned in off-site bioremediation systems. In order to find out which bacteria are active during the degradation phase in such systems, the diversity of the active microflora in a degrading soil remediation system was investigated by small-subunit (SSU) rRNA analysis. Two sequential RNA extracts from one soil sample were generated by a procedure incorporating bead beating. Both extracts were analysed separately by generating individual SSU rDNA clone libraries from cDNA of the two extracts. The sequencing results showed moderate diversity. The two clone libraries were dominated by Gammaproteobacteria, especially Pseudomonas spp. Alphaproteobacteria and Betaproteobacteria were two other large groups in the clone libraries. Actinobacteria, Firmicutes, Bacteroidetes and Epsilonproteobacteria were detected in lower numbers. The obtained sequences were predominantly related to genera for which cultivated representatives have been described, but were often clustered together in the phylogenetic tree, and the sequences that were most similar were originally obtained from soils and not from pure cultures. Most of the dominant genera in the clone libraries, e.g. Pseudomonas, Acinetobacter, Sphingomonas, Acidovorax and Thiobacillus, had already been detected in (mineral oil hydrocarbon) contaminated environmental samples. The occurrence of the genera Zymomonas and Rhodoferax was novel in mineral oil hydrocarbon-contaminated soil. INTRODUCTIONThe contamination of soils with mineral oil hydrocarbons is a widespread environmental problem. Strictly speaking, mineral oil hydrocarbons are not xenobiotic, but their largescale use and various applications lead in many cases to environmental contamination (Gallego et al., 2001). Such contamination may be a consequence of petroleum transport, storage and refining, or accidents (Juteau et al., 2003). Soil is an environmental compartment which is renewable only within long time periods, or even not renewable at all. Therefore, it is important to protect soil from such contamination or to clean contaminated soils. After treatment, the soil should be usable, at least for applications with a low purity requirement, such as road construction or the recultivation of industrial areas.One possibility for cleaning such soils is bioremediation (Juteau et al., 2003), which aims at the biological mineralization of organic compounds to CO 2 and water, or at least at transformation to less-toxic or innocuous forms (Plaza et al., 2003). The success of biodegradation depends on the predominant environmental conditions, on the chemical structure of the pollutants, on the bioavailability of the contaminating compounds, and thus on the interaction between pollutant, soil matrix and microorganisms (Del'Arco & de França, 2001;Volkering et al., 1998). Another problem of contaminated sites is the wide variety of mineral oil hydrocarbons (Li et al., 2004).There are many known consortia of micro-organisms which are able to degrade mineral oil hydro...

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Ubiquitous Acinetobacter species as beneficial commensals but gradually being emboldened with antibiotic resistance genes

Adegoke

Tom

Okoh

2012

J. Basic Microbiol.

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Acinetobacter spp. are ubiquitous obligate aerobic bacteria which occur mostly as commensals on the skin, and in soil, water and plants' rhizosphere. Though the species in this genus have been implicated as aetiologies in some nosocomial infections, their versatility covers biodegradation or dissolution leading to bioremediation; catalysis leading to synthesis of high molecular weight, life sustaining polymers; and as growth enhancers in agriculture. The challenge of antibiotic resistance and their mediatory genes is a cause for concern but should not deter the beneficial application of the bacteria especially in the synthesis of novel compounds that would be of relevance in overcoming some global ecological challenges. This review addresses important beneficial attributes of Acinetobacter species as well as gives some insight into emerging trends in their resistance to antibiotics.

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Response of the soil bacterial community to the addition of toluene and toluene-degrading bacteria

Cited by 16 publications

References 41 publications

Phylogenetic Characterization and Community Diversity of Hydrocarbon-Utilizing Bacteria in Soil Microcosms Enriched with Aromatic Hydrocarbons

Phylogenetic Characterization and Community Diversity of Hydrocarbon-Utilizing Bacteria in Soil Microcosms Enriched with Aromatic Hydrocarbons

Bacterial diversity in the active stage of a bioremediation system for mineral oil hydrocarbon-contaminated soils

Ubiquitous Acinetobacter species as beneficial commensals but gradually being emboldened with antibiotic resistance genes

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