Response of microbial communities of karst river water to antibiotics and microbial source tracking for antibiotics

Xiang, Shizheng; Wang, Xusheng; Ma, Wen; Li, Xiaoping; Zhang, Biao; Huang, Fujian; Liu, Fei; Guan, Xiangyu

doi:10.1016/j.scitotenv.2019.135730

Cited by 45 publications

(16 citation statements)

References 42 publications

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“…Antibiotic resistance is a well-recognized major threat to global public health. Many studies have surveyed antibiotic resistant bacterial strains or their corresponding genes in hotspots or reservoirs of antimicrobial resistance such as hospitals, waste-water treatment plants (WWTP), sewage systems, and animal feeding operations, where antibiotics are commonly found at high concentrations ( Bengtsson-Palme et al, 2018 ; Burcham et al, 2019 ; Kraemer et al, 2019 ; Maestre-Carballa et al, 2019 ; Xiang et al, 2020 ). In particular, attempts have been made to correlate the concentration of antibiotics with the occurrence of antibiotic resistant bacterial strains or genes in these hotspots ( Rodriguez-Mozaz et al, 2015 ; Pärnänen et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Environmental Biofilms as Reservoirs for Antimicrobial Resistance

et al. 2021

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Characterizing the response of microbial communities to a range of antibiotic concentrations is one of the strategies used to understand the impact of antibiotic resistance. Many studies have described the occurrence and prevalence of antibiotic resistance in microbial communities from reservoirs such as hospitals, sewage, and farm feedlots, where bacteria are often exposed to high and/or constant concentrations of antibiotics. Outside of these sources, antibiotics generally occur at lower, sub-minimum inhibitory concentrations (sub-MICs). The constant exposure to low concentrations of antibiotics may serve as a chemical “cue” that drives development of antibiotic resistance. Low concentrations of antibiotics have not yet been broadly described in reservoirs outside of the aforementioned environments, nor is the transfer and dissemination of antibiotic resistant bacteria and genes within natural microbial communities fully understood. This review will thus focus on low antibiotic-concentration environmental reservoirs and mechanisms that are important in the dissemination of antibiotic resistance to help identify key knowledge gaps concerning the environmental resistome.

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Section: Introductionmentioning

confidence: 99%

Environmental Biofilms as Reservoirs for Antimicrobial Resistance

et al. 2021

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“…Arcobacter infection in humans causes bacteremia-associated enteritis, which is similar to the clinical symptoms of campylobacteriosis, but with a high frequency of persistent watery diarrhea [74][75][76] . Moreover, previous studies have reported that Arcobacter is positively correlated with the concentration of antibiotics 77 and can accumulate a large number of ARGs 78 . These results indicate that pathogens carrying multidrug resistance might be concentrated and released from agricultural e uents.…”

Section: Discussionmentioning

confidence: 97%

Persistence of Antibiotic Resistance from Agricultural Effluents to Surface Water Revealed in Metagenome Assembled Genomes

Kim

Seong

Cha

et al. 2022

Preprint

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BackgroundDespite the increasing concerns about antibiotic resistance genes (ARGs) in wastewaters released from livestock or fish farms into the natural environment, studies on unculturable bacteria related to the dissemination of antibiotic resistance are limited. Here, we assessed the impact of the microbial antibiotic resistome and mobilome in wastewaters released in Korean rivers by reconstructing metagenome-assembled genomes (MAGs) using a novel refining method.ResultsWe reconstructed 1,100 MAGs using Korean river and wastewater metagenome samples and presented Additional Clustering Refiner (ACR), a method that significantly reduced contamination of genome quality. Our results indicate that ARGs harbored in the MAGs were disseminated from wastewater effluents into the downstream environment of rivers. Among the effluent-derived phyla, uncultured members of the superphylum Patescibacteria harbored ARGs co-localized with mobile genetic elements. The most abundantly detected ARGs associated with mobile genetic elements in the wastewater effluents and downstream regions of the rivers were found to originate from Patescibacteria; the detected genes represented the potential for horizontal gene transfer of beta-lactam A-, sulfonamide-, and carbapenem-resistance genes. ConclusionsOur study tracked microorganisms that were transmitted from agricultural wastewater to receiving rivers through genome-resolved metagenomics. Based on the genomic analysis of MAGs, it was found that ARGs are more commonly co-localized with MGEs in agricultural wastewater than in river water. Furthermore, this suggests that members of Patescibacteria, which are relatively unknown, are carriers that disseminate ARGs into the environment, and a potential vector for disseminating ARGs in the environmental bacterial community. Overall, this study suggests that dissemination of ARGs by uncultured bacteria should be investigated further in multiple environments.

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“…The massively parallel sequencing technology requires a rigorous choice of the 16S rRNA region to accurately describe the diversity of a bacterial community (Bukin et al, 2019). In this case, amplification using different primers of specific 16S rRNA variable regions (e.g., V3-V4 or V4) can detect better quality taxonomic differences between communities, which in turn can be used to describe differences in their functioning (Bukin et al, 2019;Xiang et al, 2020).…”

Section: Methods For Studying Ar In River Watermentioning

confidence: 99%

“…Also known as high-throughput sequencing, NGS of DNA (to determine the sequence of nucleotides in a DNA section) is performed with various sequencers (e.g., Illumina, Roche 454, Ion Torrent; B. Li et al, 2015;Meziti et al, 2019;Xiang et al, 2020); high-throughput qPCR could also be useful for analyzing hundreds of ARGs in a single run (Waseem et al, 2019), including in river water samples.…”

Section: Methods For Studying Ar In River Watermentioning

confidence: 99%

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

Grenni

2022

Enviro Toxic and Chemistry

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River ecosystems are very important parts of the water cycle and an excellent habitat, food, and drinking water source for many organisms, including humans. Antibiotics are emerging contaminants which can enter rivers from various sources. Several antibiotics and their related antibiotic resistance genes (ARGs) have been detected in these ecosystems by various research programs and could constitute a substantial problem. The presence of antibiotics and other resistance cofactors can boost the development of ARGs in the chromosomes or mobile genetic elements of natural bacteria in rivers. The ARGs in environmental bacteria can also be transferred to clinically important pathogens. However, antibiotics and their resistance genes are both not currently monitored by national or international authorities responsible for controlling the quality of water bodies. For example, they are not included in the contaminant list in the European Water Framework Directive or in the US list of Water-Quality Benchmarks for Contaminants. Although ARGs are naturally present in the environment, very few studies have focused on non-impacted rivers to assess the background ARG levels in rivers, which could provide some useful indications for future environmental regulation and legislation. The present study reviews the antibiotics and associated ARGs most commonly measured and detected in rivers, including the primary analysis tools used for their assessment. In addition, other factors that could enhance antibiotic resistance, such as the effects of chemical mixtures, the effects of climate change, and the potential effects of the coronavirus disease 2019 pandemic, are discussed.

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Response of microbial communities of karst river water to antibiotics and microbial source tracking for antibiotics

Cited by 45 publications

References 42 publications

Environmental Biofilms as Reservoirs for Antimicrobial Resistance

Environmental Biofilms as Reservoirs for Antimicrobial Resistance

Persistence of Antibiotic Resistance from Agricultural Effluents to Surface Water Revealed in Metagenome Assembled Genomes

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

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