Response mechanism in Populus × euramericana cv. ‘74/76’ revealed by RNA-seq under salt stress

Chen, Panfei; Zuo, Lihui; Yu, Xiaoyue; Dong, Yan; Zhang, Shuang; Yang, Minsheng

doi:10.1007/s11738-018-2676-x

Cited by 9 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salinity affects all stages of Populus growth, including germination [21], vegetation growth [15,22], and sexual reproduction [23]. The percentage of seeds that germinate and the extent of leaf expansion were both reported to decline as salt concentrations increase [21].…”

Section: Ss Injurymentioning

confidence: 99%

“…Long-term SS also induces early leaf maturity, early flowering, and early tree maturation in P. alba clones, which display a greater architectural modification when exposed to high SS (0.6%) than lower SS (0.3%) [22]. When exposed to 0.6% NaCl, the heights, ground diameter, and leaf numbers of Poplar 107 were significantly reduced, while plants exposed to 0.3% NaCl stress had relatively minor phenotypic changes [15]. When P. euphratica was exposed to 300 mM (1.76%) NaCl stress, the three growth indexes (plant height, ground diameter, and leaf number) were reduced to 31%, 45.5%, and 20% of the control plants, respectively.…”

Section: Ss Injurymentioning

confidence: 99%

“…The contents of Chla and Chlb, as well as the relative electron transport rate, decreased significantly in poplar 107 (a superior variety selected from Populus × euramericana cv. ’74/76′ hybrids) under 0.6% NaCl but increased under 0.3% NaCl [15].…”

Section: Ss Injurymentioning

confidence: 99%

“…Previous research primarily focused on the anatomical, physiological, and biochemical changes in poplars during SS; many recent studies have focused on the molecular mechanisms using new techniques, such as genome-scale transcript analysis [14], high-throughput sequencing [15], metabolite profiling [16], bioinformatic analyses [17,18,19], and a non-invasive micro-test technique (NMT) [20].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress

Zhang

Liu

Chen

et al. 2019

IJMS

View full text Add to dashboard Cite

Salt stress (SS) has become an important factor limiting afforestation programs. Because of their salt tolerance and fully sequenced genomes, poplars (Populus spp.) are used as model species to study SS mechanisms in trees. Here, we review recent insights into the physiological and molecular responses of Populus to SS, including ion homeostasis and signaling pathways, such as the salt overly sensitive (SOS) and reactive oxygen species (ROS) pathways. We summarize the genes that can be targeted for the genetic improvement of salt tolerance and propose future research areas.

show abstract

Section: Ss Injurymentioning

confidence: 99%

Section: Ss Injurymentioning

confidence: 99%

Section: Ss Injurymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress

Zhang

Liu

Chen

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Total RNA was extracted using a Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The RNA concentration was measured by a Nano Drop ultradifferential photometer (ThermoFisher, USA), and the quality of RNA was detected by 1% agarose gel electrophoresis to ensure that the sequencing samples were qualified (Chen et al, 2018). 1 μg total RNA was used for reverse transcription reaction using a RocketScript RT PreMix (Bioneer, Seoul, Korea).…”

Section: Total Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Overexpression of TaBADH increases salt tolerance in Arabidopsis

Sun

Liu

et al. 2019

Can. J. Plant Sci.

View full text Add to dashboard Cite

Soil salinization is an important threat to wheat growth and production. Previous transcriptome analysis showed that the expression of the betaine aldehyde dehydrogenase (BADH) gene differed significantly between cultivars with strong or weak salinity tolerance. Herein, the BADH gene from the wheat cultivar Dongnongdongmai 1 was cloned and transformed into wild-type Arabidopsis to identify its function in salt tolerance. Root length was calculated at 0, 50, 100, 150, and 200 mmol L−1 NaCl for 7 d. The relative electrolytic leakage (REL), GB content, and BADH activity were measured at 150 mmol L−1 NaCl for 1 and 3 d. It was determined that BADH activity and the GB content of TaBADH-overexpressed transgenic (TaBADHOE) lines were significantly higher than in wild-type lines. Salt stress analysis showed that the root length of TaBADHOE lines 4, 18, and 19 were 0.44, 0.54, and 0.35 cm, respectively, which were significantly longer than the 0.24 cm roots of the wild-type line in the media containing 150 mmol L−1 NaCl for 7 d. In addition, the RELs of transgenic lines 4, 18, and 19 were 0.37, 0.33, and 0.42, respectively, which is significantly lower than the 0.63 of the wild-type line in media containing 150 mmol L−1 NaCl for 3 d. These results demonstrate that TaBADH significantly increased plant salt tolerance, indicating that genetic transformation of TaBADH may be an effective and sustainable breeding method for increasing salt tolerance in wheat cultivars.

show abstract

Mass spectrometry‐based forest tree metabolomics

Rodrigues

Miguel

Chaves

et al. 2019

Mass Spectrometry Reviews

View full text Add to dashboard Cite

Research in forest tree species has advanced slowly when compared with other agricultural crops and model organisms, mainly due to the long‐life cycles, large genome sizes, and lack of genomic tools. Additionally, trees are complex matrices, and the presence of interferents (e.g., oleoresins and cellulose) challenges the analysis of tree tissues with mass spectrometry (MS)‐based analytical platforms. In this review, advances in MS‐based forest tree metabolomics are discussed. Given their economic and ecological significance, particular focus is given to Pinus, Quercus, and Eucalyptus forest tree species to better understand their metabolite responses to abiotic and biotic stresses in the current climate change scenario. Furthermore, MS‐based metabolomics technologies produce large and complex datasets that require expertize to adequately manage, process, analyze, and store the data in dedicated repositories. To ensure that the full potential of forest tree metabolomics data are translated into new knowledge, these data should comply with the FAIR principles (i.e., Findable, Accessible, Interoperable, and Re‐usable). It is essential that adequate standards are implemented to annotate metadata from forest tree metabolomics studies as is already required by many science and governmental agencies and some major scientific publishers. © 2019 John Wiley & Sons Ltd. Mass Spec Rev 40:126–157, 2021.

show abstract

Response mechanism in Populus × euramericana cv. ‘74/76’ revealed by RNA-seq under salt stress

Cited by 9 publications

References 28 publications

Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress

Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress

Overexpression of TaBADH increases salt tolerance in Arabidopsis

Mass spectrometry‐based forest tree metabolomics

Contact Info

Product

Resources

About