Respirofermentative metabolism in Kluyveromyces lactis:

González-Siso, María Isabel; Freire-Picos, María Ángeles; Ramil, Elvira; González‐Domínguez, M.; Torres, Anthony R.; Cerdán, M. Esperanza

doi:10.1016/s0141-0229(00)00161-7

Cited by 90 publications

(60 citation statements)

References 48 publications

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“…Based on this result, we could confirm that the lactose concentration determines the switch from a respiratory to a fermentative metabolism in K. marxianus UFV-3. In addition to the sugar concentration, low oxygen availability is important for bypassing the metabolic flow to fermentation in respiro-fermentative yeasts, such as the Kluyveromyces strains (González-Siso et al 1996;Silveira et al 2005). The sugar concentration and oxygen levels had a strong effect on the fermentative metabolism of K. marxianus UFV-3, which generally showed ethanol yields and ethanol volumetric productivities that are higher than those of K. lactis JA6.…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, few studies have attempted to elucidate the reasons why some strains of K. marxianus have a high fermentative metabolism. The dynamics of the switch from a respiratory to a fermentative metabolism involves the alteration of gene expression (González-Siso et al 1996). Thus, we hypothesise that comparing the transcription of the genes that are essential for lactose metabolism under aerobic and hypoxic conditions could provide a better understanding of the fermentative metabolism of K. marxianus.…”

Section: Discussionmentioning

confidence: 99%

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The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes

Diniz

Silveira

Fietto

et al. 2011

Antonie van Leeuwenhoek

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The aim of this work was to obtain insights about the factors that determine the lactose fermentative metabolism of Kluyveromyces marxianus UFV-3. K. marxianus UFV-3 and Kluyveromyces lactis JA6 were cultured in a minimal medium containing different lactose concentrations (ranging from 0.25 to 64 mmol l(-1)) under aerobic and hypoxic conditions to evaluate their growth kinetics, gene expression and enzymatic activity. The increase in lactose concentration and the decrease in oxygen level favoured ethanol yield for both yeasts but in K. marxianus UFV-3 the effect was more pronounced. Under hypoxic conditions, the activities of β-galactosidase and pyruvate decarboxylase from K. marxianus UFV-3 were significantly higher than those in K. lactis JA6. The expression of the LAC4 (β-galactosidase), RAG6 (pyruvate decarboxylase), GAL7 (galactose-1-phosphate uridylyltransferase) and GAL10 (epimerase) genes in K. marxianus UFV-3 was higher under hypoxic conditions than under aerobic conditions. The high expression of genes of the Leloir pathway, LAC4 and RAG6, associated with the high activity of β-galactosidase and pyruvate decarboxylase contribute to the high fermentative flux in K. marxianus UFV-3. These data on the fermentative metabolism of K. marxianus UFV-3 will be useful for optimising the conversion of cheese whey lactose to ethanol.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes

Diniz

Silveira

Fietto

et al. 2011

Antonie van Leeuwenhoek

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“…Saccharomyces cerevisiae and Kluyveromyces lactis are two related yeast models widely used for biotechnological purposes, with interesting metabolic differences with respect to their growth ability in different carbon sources and oxygen regulation [7]. Regarding the carbon source in S. cerevisiae, there is strong transcriptional regulation by the repression of genes involved in respiratory-related functions when cells are grown in glucose.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional repression by Kluyveromyces lactis Tup1 in Saccharomyces cerevisiae

Lamas-Maceiras

Freire-Picos

Torres

2010

J Ind Microbiol Biotechnol

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The general repression complex, constituted by the yeast Tup1 and Ssn6 factors, is a conserved global regulator of transcription in eukaryotes. In the yeast Saccharomyces cerevisiae, it is an important repressor of hypoxic genes, such as ANB1, under aerobic conditions and deletion of the TUP1 gene causes a flocculation phenotype. The KlTUP1 gene from the yeast Kluyveromyces lactis encodes for a protein with 83% similarity to Tup1 in S. cerevisiae. Despite the general domain conservation, the database searches showed the absence of a characteristic Tup1 glutamine-rich domain (Q1 at positions 96-116). Instead, there was a non-conserved sequence lacking the α-helix structure in this region. The ability to act as a transcriptional repressor was tested by expressing the KlTUP1 gene, in both high- and low-copy vectors, in an S. cerevisiae tup1 mutant strain. Repression effects were studied using the aerobic repressible reporter ANB1-lacZ and the effect on flocculation. In both regulatory systems, low levels of KlTup1 caused moderate (~30%) repression, but when the number of KlTup1 copies was increased, only the ANB1 reporter raised the repression levels of S. cerevisiae Tup1. These results show the capability of KlTup1 to act as a repressor in S. cerevisiae. The lower repression reached in S. cerevisiae is discussed in terms of structural differences.

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“…Saccharomyces cerevisiae is Crabtree-positive and thereby favours fermentation under aerobic conditions potentially limiting its biomass yield. Kluyveromyces lactis is Crabtreenegative and prefers to respire even in the presence of large concentrations of fermentable sugars, such as glucose (Breunig et al 2000;Gonzalez-Siso et al 2000). It is possible to efficiently export heterologous proteins from K. lactis by taking advantage of its well-developed killer toxin secretory apparatus (Stark et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression of cyan and yellow fluorescent proteins from the Kluyveromyces lactis KlMAL21–KlMAL22 bi-directional promoter

2007

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We have identified the Kluyveromyces lactis maltase (KlMAL22) and maltose permease (KlMAL21) intergenic region as a candidate bi-directional promoter for heterologous gene expression. The expressions of cyan and yellow fluorescent proteins from, respectively, the KlMAL22 and KlMAL21 orientations of the promoter, were compared between two promoter variants during growth in media containing glucose, galactose or glycerol. Expression from both orientations of the native promoter was repressed during growth in glucose and galactose and was induced during growth in glycerol. Disruption of a putative Mig1p binding site caused some de-repression of the maltase orientation of the promoter by 48 h of growth in glucose. The KlMAL21-KlMAL22 bi-directional promoter can be used to carry out regulated expression of heterologous gene products.

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Respirofermentative metabolism in Kluyveromyces lactis:

Cited by 90 publications

References 48 publications

The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes

The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes

Transcriptional repression by Kluyveromyces lactis Tup1 in Saccharomyces cerevisiae

Heterologous expression of cyan and yellow fluorescent proteins from the Kluyveromyces lactis KlMAL21–KlMAL22 bi-directional promoter

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