Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli

Soares, Beatriz Dugaich; Brito, Kelly Cristina Tagliari de; Grassotti, Tiela Trapp; Filho, Hiran Castagnino Kunert; Camargo, Taiara Carolaine Leal de; Carvalho, Daiane; Dorneles, Izabela Camilotti; Otutumi, Luciana Kazue; Cavalli, Lissandra Souto; Brito, Benito Guimarães de

doi:10.1016/j.cimid.2021.101700

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Salmonella outbreaks linked to backyard poultry in the United States increased during the COVID-19 pandemic in 2020 [ 2 ]. Avian pathogenic Escherichia coli (APEC) O2, a predominant serogroup of extraintestinal pathogenic E. coli , causes severe systemic infectious diseases in poultry because multidrug-resistant E. coli can live in chicken respiratory microbiota, which forms competitive exclusion of normal bacteria [ 3 ]. As a result, immunization against salmonellosis and colibacillosis is still required in poultry farms.…”

Section: Introductionmentioning

confidence: 99%

The effect of O-antigen length determinant wzz on the immunogenicity of Salmonella Typhimurium for Escherichia coli O2 O-polysaccharides delivery

Han

Luo

Zeng

et al. 2023

Vet Res

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Attenuated Salmonella Typhimurium is a promising antigen delivery system for live vaccines such as polysaccharides. The length of polysaccharides is a well-known key factor in modulating the immune response induced by glycoconjugates. However, the relationship between the length of Lipopolysaccharide (LPS) O-antigen (OAg) and the immunogenicity of S. Typhimurium remains unclear. In this study, we assessed the effect of OAg length determined by wzzST on Salmonella colonization, cell membrane permeability, antimicrobial activity, and immunogenicity by comparing the S. Typhimurium wild-type ATCC14028 strain to those with various OAg lengths of the ΔwzzST mutant and ΔwzzST::wzzECO2. The analysis of the OAg length distribution revealed that, except for the very long OAg, the short OAg length of 2–7 repeat units (RUs) was obtained from the ΔwzzST mutant, the intermediate OAg length of 13–21 RUs was gained from ΔwzzST::wzzECO2, and the long OAg length of over 20 RUs was gained from the wild-type. In addition, we found that the OAg length affected Salmonella colonization, cell permeability, and antibiotic resistance. Immunization of mice revealed that shortening the OAg length by altering wzzST had an effect on serum bactericidal ability, complement deposition, and humoral immune response. S. Typhimurium mutant strain ΔwzzST::wzzECO2 possessed good immunogenicity and was the optimum option for delivering E. coli O2 O-polysaccharides. Furthermore, the attenuated strain ATCC14028 ΔasdΔcrpΔcyaΔrfbPΔwzzST::wzzECO2-delivered E. coli O2 OAg gene cluster outperforms the ATCC14028 ΔasdΔcrpΔcyaΔrfbP in terms of IgG eliciting, cytokine expression, and immune protection in chickens. This study sheds light on the role of OAg length in Salmonella characteristics, which may have a potential application in optimizing the efficacy of delivered polysaccharide vaccines.

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Section: Introductionmentioning

confidence: 99%

The effect of O-antigen length determinant wzz on the immunogenicity of Salmonella Typhimurium for Escherichia coli O2 O-polysaccharides delivery

Han

Luo

Zeng

et al. 2023

Vet Res

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“…Los microorganismos patógenos se caracterizan por la capacidad de producir daño leve o grave mediado estrictamente por las condiciones del hospedero que lo albergue (inmunosuprimido o inmunocompetente) (Martín et al, 2018). Los factores de virulencia emergen una vez el patógeno supera los mecanismos fisiológicos de respuesta inmune del organismo a infectar y se definen como una medida cuantitativa de la gravedad y número de microorganismos necesarios para causar una enfermedad, es decir, es el grado de patogenicidad (Bag et al, 2021;Soares et al, 2021). Debido a la eficacia de los mecanismos se considera que una bacteria puede ser poco o muy virulenta, estos se identifican en dos grupos de fase temprana y de fase tardía (Nüesch et al, 2021).…”

Section: Introductionunclassified

Genes y expresión de factores de virulencia en Escherichia coli aislada en animales de producción

2022

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Los factores de virulencia de patógenos se expresan una vez superan los mecanismos fisiológicos de respuesta inmune por parte del organismo, Escherichia coli (E. coli) es una bacteria que se considera un problema en salud pública en todo el mundo debido a la alta prevalencia de mecanismos de resistencia y patogenicidad que expresa, se relaciona principalmente con infecciones intestinales y es transmitida en la cadena alimenticia; los determinantes genéticos que codifican factores de virulencia se trasladan entre diferentes especies o la misma, mediante el mecanismo de transferencia horizontal de genes. El objetivo de la presente revisión es describir factores de virulencia y genes que los codifican en cepas de E. coli aisladas de animales de producción y de productos alimenticios. En las bases de datos Medline, Lilacs, ScienceDirect, Scopus, SciELO y Dialnet, se realizó búsqueda utilizando una combinación de palabras claves validadas en inglés (gen, virulence, virulence factor, infection, horizontal gene transfer, mutation and production animals). En diferentes ambientes de producción se identificó la presencia de diferentes mecanismos de virulencia que varían según la especie animal y bacteriana, los genes con mayor circulación con Stx1, Stx2 codificantes de toxinas, Saa de adhesinas, ehxA de enterohemolisina, eaeA de intimina, IpfA de fimbrias. La vigilancia y control microbiológico en el área alimenticia y de producción animal es de gran importancia para evitar posibles brotes de enfermedades en población susceptible, por efecto de los factores de virulencia.

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Phenotypic and genotypic characterizations of bacteria isolated from the respiratory microbiota of healthy turkeys with potential for probiotic composition

Trintinaglia,

de Brito,

Kobayashi

et al. 2023

Vet Res Commun

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Respiratory microbiota of healthy broilers can act as reservoirs for multidrug-resistant Escherichia coli

Cited by 3 publications

References 19 publications

The effect of O-antigen length determinant wzz on the immunogenicity of Salmonella Typhimurium for Escherichia coli O2 O-polysaccharides delivery

The effect of O-antigen length determinant wzz on the immunogenicity of Salmonella Typhimurium for Escherichia coli O2 O-polysaccharides delivery

Genes y expresión de factores de virulencia en Escherichia coli aislada en animales de producción

Phenotypic and genotypic characterizations of bacteria isolated from the respiratory microbiota of healthy turkeys with potential for probiotic composition

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