1991
DOI: 10.1021/bi00109a014
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Abstract: New resonance Raman (RR) spectra at 15 K are reported for poplar (Populus nigra) and oleander (Oleander nerium) plastocyanins and for Alcaligenes faecalis pseudoazurin. The spectra are compared with those of other blue copper proteins (cupredoxins). In all cases, nine or more vibrational modes between 330 and 460 cm-1 can be assigned to a coupling of the Cu-S(Cys) stretch with Cys ligand deformations. The fact that these vibrations occur at a relatively constant set of frequencies is testimony to the highly co… Show more

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Cited by 107 publications
(157 citation statements)
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“…Thus, the Fe-N imid , Fe-S stretching vibrations and bond angle bending displacements are probably extensively coupled in the polypeptide backbone and widely spread into the molecule around the Rieske-type [2Fe-2S] cluster as reported previously for blue copper proteins and rubredoxin (57)(58)(59). Importantly the liganding histidine imidazole groups do not behave as simple point groups in the high and low potential Rieske-type [2Fe-2S] system, and the previous tentative assignments and interpretations of pH dependence in the RR features of high potential Rieske proteins (17,20,29) H64C variant (B).…”
Section: Probing the Novel [2fe-2s] Cluster Surroundings In The Oxidimentioning
confidence: 57%
See 1 more Smart Citation
“…Thus, the Fe-N imid , Fe-S stretching vibrations and bond angle bending displacements are probably extensively coupled in the polypeptide backbone and widely spread into the molecule around the Rieske-type [2Fe-2S] cluster as reported previously for blue copper proteins and rubredoxin (57)(58)(59). Importantly the liganding histidine imidazole groups do not behave as simple point groups in the high and low potential Rieske-type [2Fe-2S] system, and the previous tentative assignments and interpretations of pH dependence in the RR features of high potential Rieske proteins (17,20,29) H64C variant (B).…”
Section: Probing the Novel [2fe-2s] Cluster Surroundings In The Oxidimentioning
confidence: 57%
“…4, B and D) showed the expected mass-dependent downshifts by 1-2 cm Ϫ1 for most vibrational modes in the 240 -390 cm Ϫ1 region. This demonstrates the extensive kinematic mixing of the Fe-S and Fe-N imid stretching characters in the 240 -390 cm Ϫ1 region, which is expected if the Fe-S and Fe-N imid stretching vibrations are extensively coupled with other vibrations in the immediate cluster environment in the molecule involving complicated deformational displacements in the polypeptide backbone (57)(58)(59)(60). In the 390 -440 cm Ϫ1 region, a contribution of Fe-S b stretching characters becomes dominant, 7 showing no significant mass-dependent downshift with the uniformly 15 N-labeled proteins (Fig.…”
Section: Resultsmentioning
confidence: 81%
“…In particular, intense peaks , which constitute in general the fingerprint of the RR spectra of T1 copper sites, can be assigned to the mixing of the Cu-S(Cys) stretching vibration with multiple heavy atom bending modes of the ligand and adjacent residues [30][31][32][33]. POXC RR vibrational features are rather similar to those observed in some laccases and blue copper proteins, characterized by similar organization of the copper active site [30][31][32][33][34]. Slight modulations in the positions of the main peaks could be related to differences in the T1 copper site as well as in the surrounding protein matrix.…”
Section: Resultsmentioning
confidence: 99%
“…The 'blue' copper protein plastocyanin has been well characterized by crystallographic structure analysis Gewirth, Cohen, Schugar & Solomon, 1987), vibrational spectoscopy (Woodruff, Norton, Swanson & Fry, 1984;Blair et al, 1985;Han et al, 1991), X-ray absorption spectroscopy (Scott, Hahn, Doniach, Freeman & Hodgson, 1982;Penner-Hahn, Murata, Hodgson & Freeman, 1989;Murphy, Hasnain, Strange, Harvey & Ingledew, 1990), elecrochemistry (Bfichi, Bond, Codd, Huq & Freeman, 1992) and kinetic measurements (Jackman, McGinnis, Powls, Salmon & Sykes, 1988;Kyritsis et al, 1991). A quantum-mechanical treatment of the 'blue' copper site is available (Gewirth & Solomon, 1988).…”
Section: Introductionmentioning
confidence: 99%