Resonance Raman measurements of carotenoids using light-emitting diodes

Bergeson, Scott; Peatross, Justin; Eyring, Nicholas J.; Fralick, John F.; Stevenson, Douglas N.; Ferguson, Scott B.

doi:10.1117/1.2952075

Cited by 16 publications

(9 citation statements)

References 27 publications

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“…This was facilitated by a recently developed relatively inexpensive instrument configuration that uses excitation with a spectrally narrowed 473 nm LED light source instead of a 488 nm laser, and also by RRS light detection via filter instead of spectrograph. 75 However, production has not been similarly scaled up for higher-grade research devices for use in medical and public health research 76 and devices adequate for research remain relatively costly at this time. Recognizing that the solid state lasers used in portable research-grade RRS devices are still relatively expensive, we have been exploring alternative optical methodologies to assess skin carotenoid status noninvasively but at reduced cost.…”

Section: 0 Future Needsmentioning

confidence: 99%

Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies

Mayne

Cartmel

Scarmo

et al. 2013

Archives of Biochemistry and Biophysics

100

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Resonance Raman Spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of this biomarker, including its reproducibility, validity, feasibility for use in field settings, and factors that affect the biomarker such as diet, smoking, and adiposity. Recent studies have evaluated the response of the biomarker to controlled carotenoid interventions, both supplement-based and dietary [e.g., provision of a high-carotenoid fruit and vegetable (F/V)-enriched diet], demonstrating consistent response to intervention. The totality of evidence supports the use of skin carotenoid status as an objective biomarker of F/V intake, although in the cross-sectional setting, diet explains only some of the variation in this biomarker. However, this limitation is also a strength in that skin carotenoids may effectively serve as an integrated biomarker of health, with higher status reflecting greater F/V intake, lack of smoking, and lack of adiposity. Thus, this biomarker holds promise as both a health biomarker and an objective indicator of F/V intake, supporting its further development and utilization for medical and public health purposes.

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Section: 0 Future Needsmentioning

confidence: 99%

Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies

Mayne

Cartmel

Scarmo

et al. 2013

Archives of Biochemistry and Biophysics

100

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“…The enhancement effect of resonance Raman scattering has also been used to simplify the standard Raman spectrometer [202]. With the strong signal from the target molecule (carotenoids in this case), less excitation power is necessary.…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

254

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…13 It is optimized for detecting the 1525 cm −1 Raman line in skin tissue using resonance excitation. Using LEDs with interference filters gives us the flexibility to choose the excitation wavelength more or less arbitrarily.…”

Section: Optical Layoutmentioning

confidence: 99%

Divided shifted Raman spectroscopy for carotenoid detection

Bergeson

Peatross²,

Eyring³

et al. 2008

SPIE Proceedings

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We have developed a compact portable instrument for resonance Raman spectroscopy of carotenoid molecules in skin tissue. Our application focuses on the 1525 cm −1 Raman line common to all carotenoids. We use a divided shifted Raman spectroscopy (DSRS) technique that reduces sensitivity to detector drift and error. Two wavelength-narrowed LEDs illuminate the sample, and scattered light in four different wavelength channels is measured. This multi-spectral approach has single-photon sensitivity and compares favorably with laser-based Raman measurements in terms of accuracy, repeatability, and measurement time.

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Resonance Raman measurements of carotenoids using light-emitting diodes

Cited by 16 publications

References 27 publications

Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies

Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies

Raman spectroscopy: techniques and applications in the life sciences

Divided shifted Raman spectroscopy for carotenoid detection

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