1995
DOI: 10.1021/bi00039a024
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Abstract: Resonance Raman evidence that the thioester-linked 4-hydroxycinnamoyl chromophore of photoactive yellow protein is deprotonated in the dark state. Kim, M.; Mathies, R.A.; Hoff, W.D.; Hellingwerf, K.J. Published in: Biochemistry DOI:10.1021/bi00039a024Link to publication Citation for published version (APA):Kim, M., Mathies, R. A., Hoff, W. D., & Hellingwerf, K. J. (1995). Resonance Raman evidence that the thioesterlinked 4-hydroxycinnamoyl chromophore of photoactive yellow protein is deprotonated in the dark s… Show more

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Cited by 135 publications
(203 citation statements)
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References 16 publications
(34 reference statements)
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“…The difference spectra before and after irradiation with Ͼ450-nm light at Ϫ40°C are the difference between PYP M and PYP (PYP M /PYP spectrum), in which positive and negative peaks are attributed to PYP M and PYP, respectively (Fig. 2) (14). The PYP M /PYP spectrum reported here shows corresponding bands: negative at 1482, 1437, and 1163 cm Ϫ1 and positive at 1175 cm Ϫ1 .…”
Section: Resultsmentioning
confidence: 60%
See 1 more Smart Citation
“…The difference spectra before and after irradiation with Ͼ450-nm light at Ϫ40°C are the difference between PYP M and PYP (PYP M /PYP spectrum), in which positive and negative peaks are attributed to PYP M and PYP, respectively (Fig. 2) (14). The PYP M /PYP spectrum reported here shows corresponding bands: negative at 1482, 1437, and 1163 cm Ϫ1 and positive at 1175 cm Ϫ1 .…”
Section: Resultsmentioning
confidence: 60%
“…Crystallography at 1.4-Å resolution (7) and resonance Raman spectroscopy (14) have shown that the phenolic oxygen of the chromophore of PYP is deprotonated in the dark state. The photocycle is initiated by photon absorption, which involves isomerization of the ethylenic bond of the chromophore (15).…”
mentioning
confidence: 99%
“…Deprotonation of p-coumaric acid is also crucial in determining the position of the absorbance maximum of intact PYP. In the ground state of PYP the chromophore occurs in its deprotonated form, as proposed by Baca et al (1994), confirmed by crystallographic data (Borgstahl et al, 1995), and proven by resonance Raman measurements (Kim et al, 1995). The final red shift from 410 to 446 nm remains unexplained.…”
Section: Resultsmentioning
confidence: 64%
“…As a result, equation 4 can be written in a more compact way. (6) where 0 represents the electronic ground state. The main problem of dealing with equation 6 is that no analytic formulae for the electronic transition dipole moment, , are known.…”
Section: Theory a General Theory Of Resonance-raman Spectroscopymentioning
confidence: 99%
“…Furthermore, only transitions between vibrational states of the initial and intermediate states corresponding to intense vibronic transitions are enhanced, and this makes RR spectra easier to interpret than nRR ones. As a result, it is possible to study specific regions in complex systems by tuning the incident radiation to match the energy of the electronic transition of interest 3,6,7 . For instance, the enhancement can be applied only on the peaks due to the vibrations of a chromophore in a biomolecule, eliminating the interference from the rest of the molecule, present in the nRR spectrum.…”
Section: Introductionmentioning
confidence: 99%