We consider the Laplacian ∆ on an asymptotically hyperbolic manifold X, as defined by Mazzeo and Melrose [34]. We give pointwise bounds on the Schwartz kernel of the spectral measure for the operator (∆ − n 2 /4) 1/2 + on such manifolds, under the assumptions that X is nontrapping and there is no resonance at the bottom of the spectrum. This uses the construction of the resolvent given by Mazzeo and Melrose [34] (valid when the spectral parameter lies in a compact set), Melrose, Sá Barreto and Vasy [36] (high energy estimates for a perturbation of the hyperbolic metric) and the present authors [10] (see also [45]) in the general high-energy case.We give two applications of the spectral measure estimates. The first, following work due to Guillarmou and Sikora with the second author [19] in the asymptotically conic case, is a restriction theorem, that is, a L p (X) → L p (X) operator norm bound on the spectral measure. The second is a spectral multiplier result under the additional assumption that X has negative curvature everywhere, that is, a bound on functions F ((∆ − n 2 /4) 1/2 + ) of the square root of the Laplacian, in terms of norms of the function F . Compared to the asymptotically conic case, our spectral multiplier result is weaker, but the restriction estimate is stronger. In both cases, the difference can be traced to the exponential volume growth at infinity for asymptotically hyperbolic manifolds, as opposed to polynomial growth in the asymptotically conic setting.The pointwise bounds on the spectral measure established here will also be applied to Strichartz estimates in [8].