Resistência de populações de Aedes aegypti (L.) (Diptera: Culicidae) ao organofosforado temefós na Paraíba

Beserra, Eduardo B.; Fernandes, Carina; Queiroga, Maiene de Fátima Cordeiro de.; Castro, Francisco P. de

doi:10.1590/s1519-566x2007000200019

Cited by 35 publications

(30 citation statements)

References 3 publications

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“…aegypti to temephos in many states of Brazil like Espírito Santo , Rio de Janeiro (Braga et al 2004). Also from the Northeast region, resistance to temephos seems to be persistent as it was detected more recently in the state of Paraíba with RR (LC90) higher than 10 in three out of six counties (Beserra et al 2007), and in Ceará (Lima et al 2006). Santos, the only SP population grouped with populations from the Northeast region, is the city with the highest dengue incidence in the state, which means it has been under high selection pressure through intense use of insecticide.…”

Section: Discussionmentioning

confidence: 99%

Association of insecticide use and alteration on Aedes aegypti susceptibility status

Macoris

Andrighetti

Otrera

et al. 2007

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

Association of insecticide use and alteration on Aedes aegypti susceptibility status

Macoris

Andrighetti

Otrera

et al. 2007

Mem. Inst. Oswaldo Cruz

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“…aegypti populations with synthetic insecticides has turned into a serious problem. These populations have become highly resistant to these products, especially to organophosphates such as temephos (Beserra et al 2007). Besides, most synthetic insecticides are toxic to mammals and adversely affect the environment (Dharmagadda et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

Farias

Cavalheiro

Viana

et al. 2010

An. Acad. Bras. Ciênc.

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This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC 50 and LC 90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL), lectin (32 to 256 HU/mL) and trypsin inhibitory activity (3.64 ± 0.43 to 26.19 ± 0.05 gIT/kg of flour) The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD 50 > 0.15 ± 0.01 g/kg body weight), but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituents from primary and secondary metabolism upon Ae. aegypti.

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“…For each test, 10 groups of 10 larvae were placed in Petri dishes with 40ml of 0.02mg/l aqueous TE solution, while the control groups were placed in 40ml of water only. The larval mortality was assessed following 2h of exposure according to the procedure by Bezzerra et al 4 .…”

Section: Pretreatment With Pbo and Te Exposurementioning

confidence: 99%

“…The resistance to insecticides has contributed to an increase in A. aegypti populations and the incidence of dengue cases in Asia, and Central and South America, especially in Brazil 2,4 .…”

mentioning

confidence: 99%

Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti

Pereira

Limongi

Júnior

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Introduction: The effects of piperonyl butoxide (PBO) on the toxicity of the organophosphate temephos (TE) and the role of esterases in the resistance of Aedes aegypti to this insecticide were evaluated. Methods: A. aegypti L4 larvae susceptible and resistant to TE were pre-treated with PBO solutions in acetone at concentrations of 0.125, 0.25, 0.5, 1, and 2% for 24h and subsequently exposed to a diagnostic concentration of 0.02mg/L aqueous TE solution. The esterase activity of the larvae extracts pre-treated with varying PBO concentrations and exposed to TE for three time periods was determined. Results: At concentrations of 0.25, 0.5, 1, and 2%, PBO showed a signifi cant synergistic effect with TE toxicity. High levels of esterase activity were associated with the survival of A. aegypti L4 larvae exposed to TE only. Conclusions: The results of the biochemical assays suggest that PBO has a signifi cant inhibitory effect on the total esterase activity in A. aegypti larvae.Keywords: Temephos. Insecticide resistance. Aedes aegypti. Esterases.Dengue fever, a major viral disease, is a serious public health problem, especially in tropical countries where the climate and urban culture provide optimal conditions for the development and proliferation of its main vector, the mosquito Aedes aegypti 1 . The control of A. aegypti is primarily based on the use of chemical and biological products that are integrated with environmental management programs. Public programs aimed at controlling mosquitoes are characterized by the use of commercial insecticides, including organophosphates and pyrethroids. The organophosphate temephos (TE) was widely used in Brazil to control A. aegypti larvae until recently; however, due to reports of vector resistance to this insecticide, this method was replaced by the National Dengue Control Program. Nevertheless, TE remains on the list of insecticides to control dengue in Brazil and may be used again in the future 2,3 .The resistance to insecticides has contributed to an increase in A. aegypti populations and the incidence of dengue cases in Asia, and Central and South America, especially in Brazil 2,4 .The biochemical mechanisms involved in the development of resistance to insecticides include detoxifi cation metabolism processes. Several enzymes and enzyme systems participate in these processes, including oxidases, esterases, and transferases 5 . These enzymes allow the insect to convert an insecticide to a nontoxic form or to rapidly eliminate the compound from the body.Synergists such as piperonyl butoxide (PBO) have been employed in an attempt to overcome resistance to insecticides. Synergists are known to act as an alternative substrate, competing with the insecticide and interfering with detoxification. Synergists also act allosterically to inhibit the binding sites of esterases and multifunction oxidases (MFOs), minimizing the amount of insecticide needed to control insects and the levels of environmental contamination by pesticide residues 6 .Several studies have demonstrate...

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Resistência de populações de Aedes aegypti (L.) (Diptera: Culicidae) ao organofosforado temefós na Paraíba

Cited by 35 publications

References 3 publications

Association of insecticide use and alteration on Aedes aegypti susceptibility status

Association of insecticide use and alteration on Aedes aegypti susceptibility status

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

Effects of piperonyl butoxide on the toxicity of the organophosphate temephos and the role of esterases in the insecticide resistance of Aedes aegypti

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