Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1

Sun, Shufeng; Wang, Zhongyi; Fu, Haining; Duan, Canxing; Wang, Xiaoming; Zhu, Zhendong

doi:10.1016/j.cj.2015.07.006

Cited by 26 publications

(42 citation statements)

References 42 publications

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“…Their results have indicated that resistance to E. pisi is controlled by two single recessive genes ( er1 and er2 ) and one dominant gene ( Er3 ) [11,12,13,14]. The er1 , er2 , and Er3 genes have been mapped using linked markers [15,16,17,18,19,20,21,22,23,24,25,26,27]. The genes er1 and er2 map to pea linkage groups (LGs) VI and III, respectively [17,28].…”

Section: Introductionmentioning

confidence: 99%

“…To date, nine er1 alleles resistant to E. pisi have been identified in E. pisi -resistant pea germplasms: er1 -1 (also known as er1mut1 ) [14,21,25,40,41], er1 -2 [14,24,25], er1 -3 [14], er1 -4 [14], er1 -5 [38], er1 -6 [27], er1 -7 [26], er1 -10 (also known as er1mut2 ) [21,40,42], and er1 -11 [42,43]. Each er1 allele corresponds to a different PsMLO1 mutation site and pattern.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have focused on the identification of Chinese pea germplasms resistant to E. pisi [41,44,45,46,47,48,49]. In the Chinese pea cultivars X9002 and Xucai 1, E. pisi resistance is conferred by the er1 -2 allele [24,25,47], while in some Chinese pea landraces from Yunnan Province, E. pisi resistance is conferred by the er1 -6 allele [27,48]. E. pisi resistance in the Indian pea cultivar DDR11 is conferred by the er1 -7 allele [26].…”

Section: Introductionmentioning

confidence: 99%

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Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Sun

Deng

Duan

et al. 2019

IJMS

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Powdery mildew caused by Erysiphe pisi DC. severely affects pea crops worldwide. The use of resistant cultivars containing the er1 gene is the most effective way to control this disease. The objectives of this study were to reveal er1 alleles contained in 55 E. pisi-resistant pea germplasms and to develop the functional markers of novel alleles. Sequences of 10 homologous PsMLO1 cDNA clones from each germplasm accession were used to determine their er1 alleles. The frame shift mutations and various alternative splicing patterns were observed during transcription of the er1 gene. Two novel er1 alleles, er1-8 and er1-9, were discovered in the germplasm accessions G0004839 and G0004400, respectively, and four known er1 alleles were identified in 53 other accessions. One mutation in G0004839 was characterized by a 3-bp (GTG) deletion of the wild-type PsMLO1 cDNA, resulting in a missing valine at position 447 of the PsMLO1 protein sequence. Another mutation in G0004400 was caused by a 1-bp (T) deletion of the wild-type PsMLO1 cDNA sequence, resulting in a serine to leucine change of the PsMLO1 protein sequence. The er1-8 and er1-9 alleles were verified using resistance inheritance analysis and genetic mapping with respectively derived F2 and F2:3 populations. Finally, co-dominant functional markers specific to er1-8 and er1-9 were developed and validated in populations and pea germplasms. These results improve our understanding of E. pisi resistance in pea germplasms worldwide and provide powerful tools for marker-assisted selection in pea breeding.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Sun

Deng

Duan

et al. 2019

IJMS

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“…In China, little is known about inheritance mode and resistance genes for powdery mildew in Chinese pea germplasms, except for pea line X9002 [ 42 ] and cv. Xucai 1 [ 59 ]. Zeng et al [ 38 ] indicated that pea line X9002 was stably resistant to powdery mildew.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent PsMLO1 cDNA sequence analysis confirmed that resistance in X9002 was conferred by the er1- 2 allele [ 42 ]. Recently, using the same analysis applied for X9002, Sun et al [ 59 ] revealed that resistance in Chinese pea cv. Xucai 1 was also conferred by the er1- 2 allele.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Novel er1 Allele Conferring Powdery Mildew Resistance in Chinese Pea (Pisum sativum L.) Landraces

Sun

Wang

et al. 2016

PLoS ONE

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Pea powdery mildew, caused by Erysiphe pisi D.C., is an important disease worldwide. Deployment of resistant varieties is the main way to control this disease. This study aimed to screen Chinese pea (Pisum sativum L.) landraces resistant to E. pisi, and to characterize the resistance gene(s) at the er1 locus in the resistant landraces, and to develop functional marker(s) specific to the novel er1 allele. The 322 landraces showed different resistance levels. Among them, 12 (3.73%), 4 (1.24%) and 17 (5.28%) landraces showed immunity, high resistance and resistance to E. pisi, respectively. The other landraces appeared susceptible or highly susceptible to E. pisi. Most of the immune and highly resistant landraces were collected from Yunnan province. To characterize the resistance gene at the er1 locus, cDNA sequences of PsMLO1 gene were determined in 12 immune and four highly resistant accessions. The cDNAs of PsMLO1 from the immune landrace G0005576 produced three distinct transcripts, characterized by a 129-bp deletion, and 155-bp and 220-bp insertions, which were consistent with those of er1-2 allele. The PsMLO1 cDNAs in the other 15 resistant landraces produced identical transcripts, which had a new point mutation (T→C) at position 1121 of PsMLO1, indicating a novel er1 allele, designated as er1-6. This mutation caused a leucine to proline change in the amino acid sequence. Subsequently, the resistance allele er1-6 in landrace G0001778 was confirmed by resistance inheritance analysis and genetic mapping on the region of the er1 locus using populations derived from G0001778 × Bawan 6. Finally, a functional marker specific to er1-6, SNP1121, was developed using the high-resolution melting technique, which could be used in pea breeding via marker-assisted selection. The results described here provide valuable genetic information for Chinese pea landraces and a powerful tool for pea breeders.

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Inheritance of resistance of two pea lines to powdery mildew

2020

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The GRICAND, Andean Crops Research Group has identified two resistant pea (Pisum sativum L.) lines to powdery mildew (PM) (Erysiphe pisi): ILS6527 (resistance of foliage and pods) and UN6651 (pods only resistance). This study established the inheritance of these two distinct PM‐resistance genotypes. Resistant lines ILS6527 and UN6651 were crossed with two susceptible varieties (Andina and San Isidro) and their respective generations (F1, F2, BCr, and BCs) were evaluated for PM resistance in naturally infected field tests. Data were subjected to chi‐square (χ2) and homogeneity test. The two crosses of ILS6527 segregated in a monogenic Mendelian ratio of 3 susceptible: 1 resistant in F2 generation, and 1 susceptible: 1 resistant ratio in BCr, which indicate a recessive control of resistance. The resistance gene in ILS6527 may be allelic with er‐1. UN6651 exhibited PM resistance only in pods, the F2 and backcrosses from the crosses with UN6651 segregated in a manner indicative of recessive resistance.

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Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1

Cited by 26 publications

References 42 publications

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms

Discovery of a Novel er1 Allele Conferring Powdery Mildew Resistance in Chinese Pea (Pisum sativum L.) Landraces

Inheritance of resistance of two pea lines to powdery mildew

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