Resistance to cycloxaprid in Laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits

Abstract

The findings indicate that resistance to cycloxaprid develops slowly compared with resistance to other chemicals and without cross-resistance to chlorpyrifos or buprofezin; over-expressed Lsα1 is associated with low cycloxaprid resistance levels, but the importance of over-expressed Lsβ1 and reduced expression of Lsα8 could not be excluded. © 2017 Society of Chemical Industry.

Knockdown of a Nicotinic Acetylcholine Receptor Subunit Gene Bdorβ1 Decreases Susceptibility to Oxa-Bridged trans- instead of cis-Nitromethylene Neonicotinoid Insecticides in Bactrocera dorsalis

“…Cycloxaprid exhibited high bioactivity against a series of insect pests, such as N. lugens, Apolygus lucorum, Laodelphax striatellus, and Bemisia tabaci, − especially for the imidacloprid-resistant populations of A. gossypii and N.…”

Molecular Insights into the Insensitivity of Lepidopteran Pests to Cycloxaprid

“…The transcripts of nAChR β1 and α1 subunits were unregulated in the selected resistant strain of L. striatellus . In our transcriptomic data, the low-level transcription of six α subunits and a β1 subunit was detected.…”

“…The molecular target of CYC is the nAChRs, which are upregulated in a resistant strain of L. striatellus . However, in this study, no nAChRs were identified as DEGs (Table S1).…”

“…Transcriptomic technology is often used to study the interaction mechanisms between insects and pesticide molecules to identify the potential genes responsible for their tolerance or resistance. − The transcriptomic responses of several insect pests to CYC have been investigated, and different kinds of CYC-responsive genes have been identified. After continuous exposure to CYC, a 10-fold resistant strain of the small brown planthopper Laodelphax striatellus was selected, in which the transcripts of the nAChR α1 and β1 subunits were upregulated by 9.60-fold and 3.36-fold, respectively . For the white-backed planthopper Sogatella furcifera, 10 cytochrome P450 monooxygenase (CYP450) genes, 1 microsomal glutathione S-transferase (GST) gene, and 1 acetylcholinesterase gene were upregulated in response to a high-dose CYC treatment, whereas 5 CYP450 genes, 1 GST gene, and 2 carboxylesterase genes were upregulated in response to a low-dose CYC treatment .…”

The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ldα1 and Ldα8 of Leptinotarsa decemlineata and assembled receptors

“…Studies have demonstrated that not only decreased expression but also increased expression of nAChR subunit genes were associated with the development of resistance to neonicotinoids of insects. The expression of Lsα1 and Lsβ1 was significantly increased in cycloxaprid‐resistant and IMI‐resistant populations of L. striatellus , respectively, while Lsα8 was significantly downregulated (Zhang et al ., 2018a, 2018b). Conversely, Boα8 was found to be significantly upregulated in highly IMI‐resistant populations of B. odoriphaga , whereas Boα1 and Boβ1 were significantly downregulated (Shan et al ., 2020).…”