Resistance spectra of six elite breeding lines of upland rice to Pyricularia grisea

Prabhu, A. S.; Castro, Emilio; Araújo, Leila Garcês de; Berni, Rodrigo Fascin

doi:10.1590/s0100-204x2003000200006

Cited by 15 publications

(12 citation statements)

References 10 publications

(12 reference statements)

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“…These pathotypes were also found to be predominant in an earlier study with 72 isolates of M. oryzae collected in experimental fields of 10 upland rice cultivars during 1994-1997 (Filippi & Prabhu et al, 2001), indicating their high frequency and preexistence in upland rice cultivars. On the other hand, the predominant pathotype IF-1 among both leaf and panicle isolates of Primavera was not reported in earlier studies probably because of the small sample of isolates used in the analysis (Prabhu et al, 2003). In the present study, the M. oryzae population analysis of cv.…”

Section: Discussioncontrasting

confidence: 72%

Genetic and phenotypic diversity of Magnaporthe oryzae from leaves and panicles of rice in commercial fields in the State of Goiás, Brazil

Silva

Prabhu

Filippi

et al. 2009

Trop. plant pathol.

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Genetic and phenotypic structure of Magnaporthe oryzae populations of two upland rice cultivars was determined. Monoconidial isolates were obtained from rice blast affected fields, four from cv. BRS Bonança and four from cv. Primavera, in Goias State (2001)(2002)(2003). The pathotypes IB-41 and IB-9 were predominant in both leaf and panicle isolates of BRS Bonança and IF-1 in Primavera. A great majority of pathotypes were common to both leaf and panicle subpopulations of Bonança (42.8%) and Primavera (66.6%). The ANOVA of virulence data showed high variability within population of each cultivar. There was no significant difference in virulence pattern of isolates from leaves and panicles, independent of collection site and cultivar. The molecular characterization of isolates was done employing the rep-PCR analysis with two primer sequences from Pot2. The genetic analysis of 538 isolates showed a high genotypic diversity in both leaf and panicle pathogen populations with 103 haplotypes in Bonança and 49 in Primavera. The migration of pathotypes from leaves to panicles in each field was 70.8% and 36.6% for Primavera and BRS Bonança, respectively. The diversity of M. oryzae population was influenced by cultivar of origin. A great amount of population diversity was encountered within the same field. Keywords: Pyricularia grisea, virulence, Rep-PCR, rice blast. RESUMO Diversidade genética e fenotípica de Magnaporthe oruzae de folhas e panículas de arroz no Estado de Goiás, BrasilA estrutura genética e fenotípica da população de Magnaporthe oryzae foi estudada em folhas e panículas de duas cultivares de arroz de terras altas. Os isolados monospóricos foram obtidos em lavouras comerciais de arroz, quatro da cv. BRS Bonança e quatro da cv. Primavera, Goiás-Brasil (2001. As raças IB-41 e IB-9 foram as predominantes entre os isolados de folhas e panículas de Bonança e a raça IF-1 para Primavera. De 35 e 27 raças identificadas de Bonança (42,8%) e Primavera (66,6%) foram comum a ambas as subpopulações de folha e panículas. Um total de 15 raças não encontradas em folhas foi detectado em baixa freqüência em panícula de Bonança. A anova da virulência, mostrou elevada variabilidade dentro de subpopulação de cada cultivar. Não existiu mudança significativa no padrão de virulência dos isolados de folhas e panículas, independente do local e cultivar. A caracterização molecular dos isolados foi realizada por Rep-PCR com dois primers da seqüência Pot 2. A análise genética dos 538 isolados mostrou elevada diversidade genotípica nas populações de folha e de panículas, com 103 perfis em Bonança e 49 em Primavera. A migração de raças de folhas para panículas em cada lavoura foi de 70,8% (Primavera) e 36,5% (Bonança). A maior parte da diversidade da população foi encontrada dentro de cada lavoura.

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Section: Discussioncontrasting

confidence: 72%

Genetic and phenotypic diversity of Magnaporthe oryzae from leaves and panicles of rice in commercial fields in the State of Goiás, Brazil

Silva

Prabhu

Filippi

et al. 2009

Trop. plant pathol.

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“…Seeds from the backcross population and both parental lines were soaked in fungicide before transfer to Petri dishes containing wet filter paper. Germinated seeds were then planted in a greenhouse at 25°C in Agency Nuclear Malaysia following Prabhu et al (2003). Soil containing the recommended amount of NPK was supplied to plants sown in plastic trays (36 x 23 x 10 cm).…”

Section: Plant Materials and Breeding Strategymentioning

confidence: 99%

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC<sub>2</sub>F<sub>1</sub> backcross population

et al. 2017

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ABSTRACT. Rice (Oryza sativa L.) blast disease is one of the most destructive rice diseases in the world. The fungal pathogen, Magnaporthe oryzae, is the causal agent of rice blast disease. Development of 2 N. Hasan et al. Genetics and Molecular Research 16 (1): gmr16019280 resistant cultivars is the most preferred method to achieve sustainable rice production. However, the effectiveness of resistant cultivars is hindered by the genetic plasticity of the pathogen genome. Therefore, information on genetic resistance and virulence stability are vital to increase our understanding of the molecular basis of blast disease resistance. The present study set out to elucidate the resistance pattern and identify potential simple sequence repeat markers linked with rice blast disease. A backcross population (BC 2 F 1 ), derived from crossing MR264 and Pongsu Seribu 2 (PS2), was developed using marker-assisted backcross breeding. Twelve microsatellite markers carrying the blast resistance gene clearly demonstrated a polymorphic pattern between both parental lines. Among these, two markers, RM206 and RM5961, located on chromosome 11 exhibited the expected 1:1 testcross ratio in the BC 2 F 1 population. The 195 BC 2 F 1 plants inoculated against M. oryzae pathotype P7.2 showed a significantly different distribution in the backcrossed generation and followed Mendelian segregation based on a single-gene model. This indicates that blast resistance in PS2 is governed by a single dominant gene, which is linked to RM206 and RM5961 on chromosome 11. The findings presented in this study could be useful for future blast resistance studies in rice breeding programs.

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“…In contrast, a relatively high frequency of isolates from the cv. Maravilha were identified as pathotypes IB-41 and IB-9 (Prabhu et al, 2003). The predominance of the pathotypes IB-9 and IB-41 among isolates of Maravilha was also encountered among isolates of P. grisea retrieved from other upland rice cultivars (Filippi & Prabhu, 2001).…”

Section: Introductionmentioning

confidence: 78%

“…Primavera, whereas the pathotype IB-9 was commonly found among isolates of both cultivars Primavera and Maravilha. The pathotypes representing other groups (IA and ID) were obtained from the cultivars Primavera and Maravilha at low frequency (Prabhu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Virulence and rep-PCR analysis of Pyricularia grisea isolates from two Brazilian upland rice cultivars

et al. 2007

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The phenotypic and genetic diversity of 77 isolates of Pyricularia grisea collected from two upland rice cultivars, Maravilha and Primavera, was studied. Isolates exhibiting compatible reaction to cv.Primavera were incompatible to cv.Maravilha and vice versa, with the exception of six isolates that were compatible to both cultivars. The virulence of isolates from cv. Maravilha on 32 test genotypes of rice was significantly higher (t = 9.09, p ≤ 0.0001) than the isolates from cv.Primavera. A phenogram constructed from virulence data showed two main groups, one constituted mainly of isolates from cv.Primavera (97.6%) and the other of isolates from cv.Maravilha (91.17%). Rep-PCR analysis of isolates using two primers designed from sequences of Pot2 showed that isolates could be clustered broadly into two groups. The average similarity within a cluster of isolates from cv.Primavera was significantly greater than the average similarity among the isolates of cv.Maravilha (t = 5.37, p ≤ 0.0001). There was close correspondence between clusters based on PCR and virulence data (r = 0.48, p ≤ 0.011). The results showed that isolates of P. grisea were cultivar specific and had low phenotypic and genetic diversity.Additional keywords: Oryza sativa, rice blast, Magnaporthe grisea, pathotypes, genetic diversity. RESUMO Análise de virulência e rep-PCR de isolados de Pyricularia grisea de duas cultivares brasileiras de arroz de terras altasA diversidade fenotípica e genética de Pyricularia grisea foi avaliada em uma amostra de 77 isolados coletados das cultivares de arroz de terras altas Maravilha e Primavera. Isolados que apresentaram reações compatíveis em cv. Primavera foram incompatíveis em cv.Maravilha e vice versa, com exceção de seis isolados que foram compatíveis nas duas cultivares. A virulência de isolados provenientes da cv. Maravilha, em 32 genótipos de arroz foi significativamente maior (t = 9,09; p ≤ 0,0001) que dos isolados da cv. Primavera. O fenograma construído a partir dos dados fenotípicos mostrou dois grupos, um constituído principalmente por isolados da cv. Primavera (97,6%) e outro de isolados da cv. Maravilha (91,17%). A análise de rep-PCR dos isolados, utilizando os primers Pot2., revelou dois grupos distintos. A similaridade média dos isolados da cv. Primavera dentro de grupo foi maior do que a similaridade média dos isolados da cv. Maravilha (t = 5,37; p ≤ 0,0001). Observou-se uma correspondência entre a análise de agrupamento baseada em PCR e virulência fenotípica (r = 0,48; p ≤0,011). Os resultados mostraram ainda uma alta especificidade de isolados de P. grisea para cada cultivar e baixa variação fenotípica e genética.

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Resistance spectra of six elite breeding lines of upland rice to Pyricularia grisea

Cited by 15 publications

References 10 publications

Genetic and phenotypic diversity of Magnaporthe oryzae from leaves and panicles of rice in commercial fields in the State of Goiás, Brazil

Genetic and phenotypic diversity of Magnaporthe oryzae from leaves and panicles of rice in commercial fields in the State of Goiás, Brazil

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC<sub>2</sub>F<sub>1</sub> backcross population

Virulence and rep-PCR analysis of Pyricularia grisea isolates from two Brazilian upland rice cultivars

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