Resistance Response of the Ma genes from `Myrobalan' Plum to Meloidogyne hapla and M. mayaguensis

Rubio-Cabetas, M. J.; Minot, J. C.; Voisin, R; Esmenjaud, Daniel; Salesses, G.; Bonnet, A.

doi:10.21273/hortsci.34.7.1266

Cited by 26 publications

(12 citation statements)

References 6 publications

(9 reference statements)

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“…accession P.2175 is highly resistant to all tested RKN species (M. incognita, M. arenaria, M. javanica, M. floridensis, and Meloidogyne mayaguensis (Lecouls et al 1997;Rubio-Cabetas et al 1999)), a trait conferred by the major dominant gene Ma. Several reliable sequencecharacterized amplified region (SCAR) markers, flanking or co-segregating with the gene, are already available for the development of marker-assisted selection (Lecouls et al 2004;Esmenjaud and Dirlewanger 2007).…”

Section: Communicated By a Abbottmentioning

confidence: 99%

Characterization of the RMja gene for resistance to root-knot nematodes in almond: spectrum, location, and interest for Prunus breeding

Ghelder¹,

Lafargue

Dirlewanger

et al. 2010

Tree Genetics & Genomes

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In Prunus spp., resistance genes to root-knot nematodes (RKN), Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica, and Meloidogyne floridensis, confer either a complete spectrum, e.g., the Ma and Rjap genes in Myrobalan and Japanese plums (subgenus Prunophora), respectively, or a more restricted spectrum, e.g., the RMia gene (M. arenaria + M. incognita) in peach (subgenus Amygdalus). We report here characterization data of the RMja gene from the almond Alnem1, another Amygdalus source. The study of its spectrum is hampered by the inability of almond to be propagated by cuttings; we overcame this problem by using F1 and BC1 crosses with previously genotyped Myrobalan plums that conferred their rooting ability to hybrids for simultaneous evaluation to different RKN. As expected from a homozygous dominant resistance, BC1 progenies of Alnem1 segregated for resistance to M. javanica but were uniformly susceptible to M. incognita and M. floridensis, demonstrating that RMja controlled M. javanica but not M. incognita nor M. floridensis. SSR markers covering the Prunus reference map placed RMja on LG7 in the same region as Ma and Rjap and thus showed its independence from the RMia gene (LG2) of the botanically closer peach. The spectrum of this gene allows the theoretical construction of interspecific rootstocks, Myrobalan plum × (almond × peach), which cumulate RMja with Ma and RMia and are protected from each of the predominant RKN affecting Prunus, i.e., M. arenaria, M. incognita, and M. javanica, by at least two genes. This pyramiding strategy should offer to rootstock material an unprecedented guarantee of durable RKN resistance.

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Section: Communicated By a Abbottmentioning

confidence: 99%

Characterization of the RMja gene for resistance to root-knot nematodes in almond: spectrum, location, and interest for Prunus breeding

Ghelder¹,

Lafargue

Dirlewanger

et al. 2010

Tree Genetics & Genomes

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“…Florida (FL) (Lecouls et al 1997;Rubio-Cabetas et al 1999) and to the minor species Melpidogyne mayaguensis (Fargette et al 1996;Rubio-Cabetas et al 1999). This resistance is conferred by the Ma dominant resistance gene in the heterozygous state in P.2175 (Esmenjaud et al 1996b;Rubio-Cabetas et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid?location of root-knot nematode resistance genes

et al. 2004

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Inheritance and linkage studies were carried out with microsatellite [or simple sequence repeat (SSR)] markers in a F(1) progeny including 101 individuals of a cross between Myrobalan plum ( Prunus cerasifera Ehrh) clone P.2175 and the almond (Prunus dulcis Mill.)-peach ( Prunus persica L. Batsch) hybrid clone GN22 ["Garfi" (G) almond x "Nemared" (N) peach]. This three-way interspecific Prunus progeny was produced in order to associate high root-knot nematode (RKN) resistances from Myrobalan and peach with other favorable traits for Prunus rootstocks from plum, peach and almond. The RKN resistance genes, Ma from the Myrobalan plum clone P.2175 and R(MiaNem) from the 'N' peach, are each heterozygous in the parents P.2175 and GN22, respectively. Two hundred and seventy seven Prunus SSRs were tested for their polymorphism. One genetic map was constructed for each parent according to the "double pseudo-testcross" analysis model. The Ma gene and 93 markers [two sequence characterized amplified regions (SCARs), 91 SSRs] were placed on the P.2175 Myrobalan map covering 524.8 cM. The R(MiaNem) gene, the Gr gene controlling the color of peach leaves, and 166 markers (one SCAR, 165 SSRs) were mapped to seven linkage groups instead of the expected eight in Prunus. Markers belonging to groups 6 and 8 in previous maps formed a single group in the GN22 map. A reciprocal translocation, already reported in a G x N F(2), was detected near the Gr gene. By separating markers from linkage groups 6 and 8 from the GN22 map, it was possible to compare the eight homologous linkage groups between the two maps using the 68 SSR markers heterozygous in both parents (anchor loci). All but one of these 68 anchor markers are in the same order in the Myrobalan plum map and in the almond-peach map, as expected from the high level of synteny within Prunus. The Ma and R(MiaNem)genes confirmed their previous location in the Myrobalan linkage group 7 and in the GN22 linkage group 2, respectively. Using a GN22 F(2) progeny of 78 individuals, a microsatellite map of linkage group 2 was also constructed and provided additional evidence for the telomeric position of R(MiaNem) in group 2 of the Prunus genome.

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“…Clones P.2175, P.1079 and P.2980 of the Myrobalan plum proved to be resistant to the population Meloidogyne sp. Florida Lecouls et al 1997;Rubio-Cabetas et al 1999). All three clones carry one dominant allele of a single resistance gene, designated Ma1, Ma2 and Ma3, respectively (Esmenjaud et al 1996b;Rubio-Cabetas et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Each of these Ma resistance alleles confers a high and wide-spectrum resistance to M. arenaria, M. incognita, M. javanica and M. sp. Florida (Lecouls et al 1997;Rubio-Cabetas et al 1999) and to the minor species Meloidogyne mayaguensis (Fargette et al 1996;RubioCabetas et al 1999). This resistance was not overcome by any of the over-30 RKN species and isolates tested Fernandez et al 1994), and was not modified under conditions known as affecting plant defences to RKN such as high temperature and high inoculum pressure (Esmenjaud et al 1996a).…”

Section: Introductionmentioning

confidence: 99%

Location of independent root-knot nematode resistance genes in plum and peach

Claverie¹,

Bosselut²,

Lecouls³

et al. 2003

Theor Appl Genet

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Prunus species express different ranges and levels of resistance to the root-knot nematodes (RKN) Meloidogyne spp. In Myrobalan plum ( Prunus cerasifera), the dominant Ma gene confers a high-level and wide-spectrum resistance to the predominant RKN, Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica and the isolate Meloidogyne sp. Florida which overcomes the resistance of the Amygdalus sources. In Japanese plum ( Prunus salicina), a similar wide-spectrum dominant resistance gene, termed R(jap), has been hypothesized from an intraspecific segregating cross. In peach, two crosses segregating for resistance to both M. incognita and M. arenaria were used to identify single genes that each control both RKN species in the Shalil ( R(Mia557)) and Nemared ( R(MiaNem)) sources. Localisation of these genes was made possible using the RFLP and SSR- saturated reference Prunus map TxE, combined with a BSA approach applied to some of the genes. The Ma1 allele carried by the Myrobalan plum accession P.2175 was localised on the linkage group 7 at an approximate distance of 2 cM from the SSR marker pchgms6. In the Japanese plum accession J.222, the gene R(jap) was mapped at the same position in co-segregation with the SSR markers pchgms6 and CPPCT022. The peach genes R(Mia557) and R(MiaNem), carried by two a priori unrelated resistance sources, were co-localized in a subtelomeric position on linkage group 2. This location was different from the more centromeric position previously proposed by Lu et al. (1999) for the resistance gene Mij to M. incognita and M. javanica in Nemared, near the SSR pchgms1 and the STS EAA/MCAT10. By contrast, R(Mia557) and R(MiaNem) were flanked by STS markers obtained by Yamamoto and Hayashi (2002) for the resistance gene Mia to M. incognita in the Japanese peach source Juseitou. Concordant results for the three independent sources, Shalil, Nemared and Juseitou, suggest that these peach RKN sources share at least one major gene resistance to M. incognita located in this subtelomeric position. We showed that plum and peach genes are independent and, thus, can be pyramided into interspecific hybrid rootstocks based on the plum and peach species.

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Resistance Response of the Ma genes from `Myrobalan' Plum to Meloidogyne hapla and M. mayaguensis

Cited by 26 publications

References 6 publications

Characterization of the RMja gene for resistance to root-knot nematodes in almond: spectrum, location, and interest for Prunus breeding

Characterization of the RMja gene for resistance to root-knot nematodes in almond: spectrum, location, and interest for Prunus breeding

Microsatellite genetic linkage maps of myrobalan plum and an almond-peach hybrid?location of root-knot nematode resistance genes

Location of independent root-knot nematode resistance genes in plum and peach

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