Resistance of staphylococci in The Netherlands: surveillance by an electronic network during 1989-1995

Neeling, Albert J. de; Leeuwen, W. J. Van; Schouls, Leo M.; Schot, Corrie S.; Veen-Rutgers, A. Van; Beunders, Alexandra J.M.; Buiting, A. G. M.; Hol, C.; Ligtvoet, E. E. J.; Petit, P. L. C.; Sabbe, L.; Griethuysen, A. J. A. van; Embden, J. D. A. Van

doi:10.1093/jac/41.1.93

Cited by 89 publications

(56 citation statements)

References 23 publications

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“…Colonies suspect of being MRSA were tested by PCR for the S. aureus specific DNA fragment (Martineau et al, 1998), the mecA gene (De Neeling et al, 1998), and the Panton-Valentine leucocidin toxin genes (Lina et al, 1999).…”

Section: Identification Of Bacteriamentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus in horses and horse personnel: An investigation of several outbreaks

Duijkeren

Moleman

Oldruitenborgh‐Oosterbaan

et al. 2010

Veterinary Microbiology

161

147

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Section: Identification Of Bacteriamentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus in horses and horse personnel: An investigation of several outbreaks

Duijkeren

Moleman

Oldruitenborgh‐Oosterbaan

et al. 2010

Veterinary Microbiology

161

147

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“…All MRSA isolates were genetically characterized by PCR specific for S. aureus [22], the mecA gene [23], and the Panton-Valentine leucocidin (PVL) toxin genes [24]. Staphylococcal protein A (spa) typing was conducted according to Harmsen et al [25].…”

Section: Microbiological Examinationmentioning

confidence: 99%

Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The Netherlands

et al. 2010

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SUMMARYTo determine methicillin-resistant Staphylococcus aureus (MRSA) carriage in poultry and slaughterhouse personnel, 40 Dutch broiler flocks, in six slaughterhouses and 466 personnel were sampled. Of the employees, 26 were positive (5 . 6 %), indicating a higher risk of exposure when compared to the general Dutch population (0 . 1 %). This risk was significantly higher for personnel having contact with live animals (5 . 2 %) -especially hanging broilers on the slaughterline (20 . 0%) -than for all other personnel (1 . 9 %). Conventional electric stunning conferred a significantly higher risk of MRSA carriage for employees than CO 2 stunning (9 . 7% vs. 2 . 0%). A total of 405 broilers were sampled upon their arrival at the slaughterhouse, of which 6 . 9% were positive. These broilers originated from 40 Dutch slaughter flocks of which 35 . 0% were positive. MRSA contamination in the different compartments of slaughterhouses increased during the production day, from 8 % to 35 %. Of the 119 MRSA isolates, predominantly livestock-associated MRSA ST398 was found, although 27 . 7 % belonged to ST9 (spa type t1430). There is an increased risk of MRSA carriage in personnel working at broiler slaughterhouses, particularly those having contact with live animals.

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“…An Etest (AB Biodisk, Solna, Sweden) showed that the oxacillin MIC for the isolates of the dog and the family members was 192 mg/liter. The presence of the mecA gene was demonstrated by a positive mecA PCR for all isolates, as previously described (2). PFGE was carried out as described by Schwarzkopf et al (12).…”

Section: Case Reportmentioning

confidence: 99%

Transmission of a Panton-Valentine Leucocidin-Positive, Methicillin-Resistant Staphylococcus aureus Strain between Humans and a Dog

et al. 2005

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Panton-Valentine leucocidin-positive methicillin-resistant Staphylococcus aureus (MRSA) strains with identical resistance patterns were cultured from recurrent infections of a 51-year-old patient, her healthy husband, son, and dog, and pulsed-field gel electrophoresis showed that all MRSA strains were indistinguishable. CASE REPORTIn 2002, a 51-year-old female patient with a diabetic foot was admitted to the surgical ward of a Dutch teaching hospital in the province Overijssel. Cultures were taken from the ulcer at admission, and a methicillin-resistant Staphylococcus aureus (MRSA) strain, resistant to fusidic acid and tetracyclines but susceptible to macrolides, trimethoprim-sulfamethoxazole, quinolones, rifampin, and aminoglycosides was cultured, while cultures from nose, throat, and perineum did not grow MRSA. The patient was treated successfully with trimethoprim-sulfamethoxazole. Two months later the patient was screened for the MRSA carrier state, and a sample from her throat was culture positive for an MRSA strain with the same resistance pattern, while nostril, perineum, and wound cultures were MRSA negative. She developed a urinary tract infection with a positive MRSA culture again 4 months after the initial treatment. Therapy with ciprofloxacin and rifampin was started, after which the MRSA infection was eradicated for half a year, based on monthly negative cultures of urine, nostrils, throat, wound, and perineum. However, the final screening for carrier state after this half-year revealed an MRSA strain again in cultures from the nose, throat, and perineum. After these findings a source of the recurrent MRSA carrier state was suspected at her home. Samples were taken from the nose and throat of the patient's husband (57 years old), son (25 years old), and their dog, a German Shepherd, who were all clinically healthy. The samples from the throat of the husband and from the nose of the son and dog were culture positive for MRSA. In a last effort to eradicate the patient's MRSA infection, all family members (including the dog) were treated simultaneously with ciprofloxacin and rifampin. This last treatment eradicated the MRSA carrier state of all persons and the dog. Follow-up cultures of nostrils, throat, and perineum of all persons and the dog were taken for 6 months, and all cultures remained negative.All isolates were sent to the National Institute of Public Health and the Environment (RIVM) for identification and genotyping by pulsed-field gel electrophoresis (PFGE). The staphylococci isolated from the patient (isolates from the ulcer, throat, and urine), her husband and son, and the dog were all identified as S. aureus by conventional methods, Vitek 2 (BioMérieux, Marcy-l'Étoile, France) and Martineau PCR (8). Susceptibility testing was performed using the Vitek 2 assay according to the manufacturer's instructions. All isolates had the same resistance pattern: they were resistant to all beta-lactams, fusidic acid, and tetracycline and were susceptible to aminoglycosides, fluoroquinolones, erythromy...

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Resistance of staphylococci in The Netherlands: surveillance by an electronic network during 1989-1995

Cited by 89 publications

References 23 publications

Methicillin-resistant Staphylococcus aureus in horses and horse personnel: An investigation of several outbreaks

Methicillin-resistant Staphylococcus aureus in horses and horse personnel: An investigation of several outbreaks

Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The Netherlands

Transmission of a Panton-Valentine Leucocidin-Positive, Methicillin-Resistant Staphylococcus aureus Strain between Humans and a Dog

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