Resistance of a Halobacterium salinarum isolate from a solar saltern to cadmium, lead, nickel, zinc, and copper

Haloarchaea can survive and thrive under exposure to a wide range of extreme environmental factors, which represents a potential interest to biotechnology. Growth responses to different stressful conditions were examined in the haloarchaeon Haloferax mediterranei R4. It has been demonstrated that this halophilic archaeon is able to grow between 10 and 32.5% (w/v) of sea water, at 32–52 °C, although it is expected to grow in temperatures lower than 32 °C, and between 5.75 and 8.75 of pH. Moreover, it can also grow under high metal concentrations (nickel, lithium, cobalt, arsenic), which are toxic to most living beings, making it a promising candidate for future biotechnological purposes and industrial applications. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis quantified the intracellular ion concentrations of these four metals in Hfx. mediterranei, concluding that this haloarchaeon can accumulate Li+, Co2+, As5+, and Ni2+ within the cell. This paper is the first report on Hfx. mediterranei in which multiple stress conditions have been studied to explore the mechanism of stress resistance. It constitutes the most detailed study in Haloarchaea, and, as a consequence, new biotechnological and industrial applications have emerged.

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“…volcanii, or Hfx. vallismortis can grow up to similar concentrations [34][35][36]; however, Haloarcula sp. can only grow up to 0.1 mM of Ni [37].…”

Section: Metal Stressmentioning

confidence: 99%

The Survival of Haloferax mediterranei under Stressful Conditions

et al. 2021

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“…The strains phylogenetically characterized by sequencing PCR-amplified 16S rRNA showed their affiliation to Halobacterium salinarum . The obtained sequences were submitted to EMBL/GenBank databases under accession number MT332425, MT741679, MT738366, MT738365, and MT738367 for the strains AS1, AS2, AS8, AS11, and AS19 respectively (Baati et al 2020b ).…”

Section: Methodsmentioning

confidence: 99%

“…The strains were cultivated to extract genomic DNA, for 10 days, at 37 °C, in DSC-97 medium as described previously (Baati et al 2020b ). The cells obtained were harvested by centrifugation (8000 g for 30 min), and the genomic DNA of each strain was extracted using Genomic DNA Purification Kit (NucleoSpin Tissue Kit, macherey Nagel) according to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

“…The effect of heavy metals ions on the growth kinetics of strain AS1, selected for its high resistance to heavy metals especially cadmium and lead, has been recently studied. This strain was able to tolerate high concentrations of lead, cadmium, and nickel in liquid medium (Baati et al 2020b ). However, to date, no information exists on the genomic basis of halophilic archaea survival under variable environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

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Genomic analysis of heavy metal-resistant Halobacterium salinarum isolated from Sfax solar saltern sediments

et al. 2022

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The draft genome sequences of five archaeal strains, isolated from Sfax solar saltern sediments and affiliated with Halobacterium salinarum, were analyzed in order to reveal their adaptive strategies to live in hypersaline environments polluted with heavy metals. The genomes of the strains (named AS1, AS2, AS8, AS11, and AS19) are found to contain 2,060,688; 2,467,461; 2,236,624; 2,432,692; and 2,428,727 bp respectively, with a G + C content of 65.5, 66.0, 67.0, and 66.2%. The majority of these genes (43.69–55.65%) are annotated as hypothetical proteins. Growth under osmotic stress is possible by genes coding for potassium uptake, sodium efflux, and kinases, as well as stress proteins, DNA repair systems, and proteasomal components. These strains harbor many genes responsible for metal transport/resistance, such as: copper-translocating P-type ATPases, ABC transporter, and cobalt-zinc-cadmium resistance protein. In addition, detoxification enzymes and secondary metabolites are also identified. The results show strain AS1, as compared to the other strains, is more adapted to heavy metals and may be used in the bioremediation of multi-metal contaminated environments. This study highlights the presence of several commercially valuable bioproducts (carotenoids, retinal proteins, exopolysaccharide, stress proteins, squalene, and siderophores) and enzymes (protease, sulfatase, phosphatase, phosphoesterase, and chitinase) that can be used in many industrial applications. Supplementary Information The online version contains supplementary material available at 10.1007/s00792-022-01273-0.

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“…Since then, a number of studies were conducted on bacterial species like S. aureus, Cupriavidus metallidurans, Escherichia coli, and Bacillus subtilis for Cd-resistance (Nies et al, 1989;Nucifora et al, 1989;Rensing et al, 1997;Solovieva and Entian, 2002). More recently, strains with superior Cd tolerance were isolated for various purpose (Baati et al, 2020;Kotoky and Pandey, 2020;Minari et al, 2020;Shi et al, 2020). For example, the Cupriavidus sp.…”

Section: Introductionmentioning

confidence: 99%

Genomic Insights Into Cadmium Resistance of a Newly Isolated, Plasmid-Free Cellulomonas sp. Strain Y8

Chen

Wang

et al. 2022

Front. Microbiol.

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Our current knowledge on bacterial cadmium (Cd) resistance is mainly based on the functional exploration of specific Cd-resistance genes. In this study, we carried out a genomic study on Cd resistance of a newly isolated Cellulomonas strain with a MIC of 5 mM Cd. Full genome of the strain, with a genome size of 4.47 M bp and GC-content of 75.35%, was obtained through high-quality sequencing. Genome-wide annotations identified 54 heavy metal-related genes. Four potential Cd-resistance genes, namely zntAY8, copAY8, HMTY8, and czcDY8, were subjected to functional exploration. Quantitative PCR determination of in vivo expression showed that zntAY8, copAY8, and HMTY8 were strongly Cd-inducible. Expression of the three inducible genes against time and Cd concentrations were further quantified. It is found that zntAY8 responded more strongly to higher Cd concentrations, while expression of copAY8 and HMTY8 increased over time at lower Cd concentrations. Heterologous expression of the four genes in Cd-sensitive Escherichia coli led to different impacts on hosts’ Cd sorption, with an 87% reduction by zntAY8 and a 3.7-fold increase by HMTY8. In conclusion, a Cd-resistant Cellulomonas sp. strain was isolated, whose genome harbors a diverse panel of metal-resistance genes. Cd resistance in the strain is not controlled by a dedicated gene alone, but by several gene systems collectively whose roles are probably time- and dose-dependent. The plasmid-free, high-GC strain Y8 may provide a platform for exploring heavy metal genomics of the Cellulomonas genus.

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Resistance of a Halobacterium salinarum isolate from a solar saltern to cadmium, lead, nickel, zinc, and copper

Cited by 13 publications

References 47 publications

The Survival of Haloferax mediterranei under Stressful Conditions

The Survival of Haloferax mediterranei under Stressful Conditions

Genomic analysis of heavy metal-resistant Halobacterium salinarum isolated from Sfax solar saltern sediments

Genomic Insights Into Cadmium Resistance of a Newly Isolated, Plasmid-Free Cellulomonas sp. Strain Y8

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