Resistance Evaluation of Tomato Germplasm against Bacterial Wilt by Ralstonia solanacearum

Abstract

This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato lines for breeding purpose. We evaluated the disease responses of a total of 13 cultivars and 39 breeding lines from RDA-Genebank using R. solanacearum SL341 strain, which is a representative strain in Korea. Tomato cultivar Hawaii 7996 and Moneymaker were used as a resistant control plant and a susceptible control plant, respectively. A total of 32 cultivars were susceptible and 10 cultivars showed various disease response suggesting resistant phenotype segregation in the lines. Five commercial cultivars and 5 breeding lines exhibited strong resistance to bacterial wilt by the SL341 strain. These 5 breeding lines might be used for further study of plant defense response against bacterial wilt and cloning of the resistance gene from tomato plants. Ultimately, the selected lines could be used for tomato breeding to generate bacterial wilt resistant tomato plants.

Leaf-to-Whole Plant Spread Bioassay for Pepper and Ralstonia solanacearum Interaction Determines Inheritance of Resistance to Bacterial Wilt for Further Breeding

“…Using this root-infection method requires a wound of the root; however, there is uncertainty regarding the infections before the symptoms alongside difficulty in knowing the exact resistance phenotype depending on the degree of artificial root wound. Consequentially, variation and deviation of the BW symptom appear large in plants [ 15 , 16 , 17 , 18 ]. To overcome these problems, we developed an LWB assay for BW on peppers.…”

“…Most studies on resistance to R. solanacearum in plants used two screening methods of R. solanacearum, i.e., root cut (soil)-drench and root-dipping inoculation [ 15 , 16 , 17 , 18 ]. However, both methods are difficult to determine the resistance degree according to the size of artificial root wounds, which leads to a large standard deviation due to low uniformity after inoculation [ 17 ].…”

Leaf-to-whole plant spread bioassay for pepper andRalstonia solanacearuminteraction determines inheritance of resistance to bacterial wilt for further breeding

“…Most studies on resistance to R. solanacearum in plants have used two screening methods of R. solanacearum, such as root cut (soil)-drench and root-dipping inoculation [15][16][17][18]. However, both methods are difficult to determine the resistance degree according to the size of artificially root wounds lead to the standard deviation is large due to low uniformity after inoculation [17].…”

Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea

“…This disease has been observed not only in many economically important solanaceous crops, such as potato, tomato, and pepper plants, but also in paprika, sesame, peanut, sunflower, etc ( Jeong et al, 2007 ; Lee and Kang, 2013 ; Lim et al, 2008 ; Seo et al, 2007 ; Yun et al, 2004 ). Therefore, there have been great efforts to overcome this disease by breeding resistant varieties or detecting pathogenic bacteria using PCR-based methods ( Cho et al, 2011 ; Han et al, 2009 ; Jung et al, 2014 ; Kang et al, 2007 ; Kim et al, 2016 ; Lee et al, 2011 ).…”