Resemblance and divergence: the “new” members of the genus Bordetella

Gross, Roy; Keidel, Kristina; Schmitt, Karin

doi:10.1007/s00430-010-0148-z

Cited by 38 publications

(32 citation statements)

References 70 publications

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“…Therefore, species identification and confirmation of Bordetella spp. must occur to allow an accurate diagnosis of pertussis and pertussislike diseases in humans (9).…”

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confidence: 99%

Novel Multitarget Real-Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens

et al. 2011

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A novel multitarget real-time PCR (RT-PCR) assay for the rapid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multicopy insertion sequences (ISs) in combination with the pertussis toxin subunit S1 (ptxS1) singleplex assay. The RT-PCR targets for the multiplex assay include IS481, commonly found in B. pertussis and B. holmesii; IS1001 of B. parapertussis; and the IS1001-like sequence of B. holmesii. Overall, 402 Bordetella species and 66 non-Bordetella species isolates were tested in the multitarget assay. Cross-reactivity was found only with 5 B. bronchiseptica isolates, which were positive with IS1001 of B. parapertussis. The lower limit of detection (LLOD) of the multiplex assay was similar to the LLOD of each target in an individual assay format, which was approximately 1 genomic equivalent per reaction for all targets. A total of 197 human clinical specimens obtained during cough-illness outbreak investigations were used to evaluate the multitarget RT-PCR assay. The multiplex assay results from 87 clinical specimens were compared to the individual RT-PCR assay and culture results. The multitarget assay is useful as a diagnostic tool to confirm B. pertussis infections and to rapidly identify other Bordetella species. In conclusion, the use of this multitarget RT-PCR approach increases specificity, while it decreases the amount of time, reagents, and specimen necessary for RT-PCRs used for accurate diagnosis of pertussis-like illness.

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“…Therefore, species identification and confirmation of Bordetella spp. must occur to allow an accurate diagnosis of pertussis and pertussislike diseases in humans (9).…”

mentioning

confidence: 99%

Novel Multitarget Real-Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens

et al. 2011

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“…The genus includes Bordetella pertussis, an obligate human pathogen and the causative agent of whooping cough, and the closely related Bordetella bronchiseptica, which can infect a wide range of mammals, including several species that are commonly studied in the laboratory (1)(2)(3). These bacteria rely on the global two-component regulatory system BvgAS for virulence (1)(2)(3). The BvgAS phosphorelay regulates gene expression patterns according to environmental cues and controls at least three distinct phenotypic phases: Bvg minus (Bvg Ϫ ), Bvg plus (Bvg ϩ ), and Bvg intermediate (Bvg i ) (4,5).…”

mentioning

confidence: 99%

An Improved Recombination-Based In Vivo Expression Technology-Like Reporter System Reveals Differential cyaA Gene Activation in Bordetella Species

Byrd¹,

Mason²,

Scheller³

et al. 2013

Infect Immun

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Bordetella pertussis and Bordetella bronchiseptica rely on the global two-component regulatory system BvgAS to control expression of distinct phenotypic phases. In the Bvg ؊ phase, expression of vrg genes, including those required for motility in B. bronchiseptica, is activated and genes encoding virulence factors are not expressed. Conversely, in the Bvg ؉ phase, genes encoding virulence factors are highly expressed while genes necessary for motility are repressed. Although several genetic analyses have demonstrated the importance of the Bvg ؉ phase during respiratory infection, Bvg-regulated gene activation in B. bronchiseptica has not been investigated in vivo. To address this, we developed a plasmid, pGFLIP, that encodes a sensitive Flp recombinasebased fluorescent reporter system able to document gene activation both in vitro and in vivo. Using pGFLIP, we demonstrated that cyaA, considered to be a "late" Bvg ؉ phase gene, is activated substantially earlier in B. bronchiseptica than B. pertussis following a switch from Bvg ؊ to Bvg ؉ phase conditions. We show that the altered activation of cyaA is not due to differences in the cyaA promoter or in the bvgAS alleles of B. bronchiseptica compared to B. pertussis, but appears to be species specific. Finally, we used pGFLIP to show that flaA remains repressed during infection, confirming that B. bronchiseptica does not modulate to the Bvg ؊ phase in vivo.

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“…Mechanisms underlying pathogenicity of the virus have been reviewed [44] with particular reference to the problem of virus-induced CNS disease [45]. Molecular pathways to pathogen variation and adaptation to the host have been reviewed for E. coli [46] (16), Bordetella [47], and Neisseria [48,49]. Of related interest are the regulation of white-opaque switch in Candida albicans [50] and the role of evolutionary conserved signaling systems in Echinococcus with regard to host-parasite interaction [51].…”

Section: Pathogen Variation and Host Response In Infectious Diseasesmentioning

confidence: 99%

Recent publications in medical microbiology and immunology: a retrospective

Doerr

Cinatl

2011

Med Microbiol Immunol

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A look back is done to some clinical and basic research activities recently published in medical microbiology and immunology. The review covers clinical experiences and in vitro experiments to understand the emergency, pathogenicity, epidemic spread, and vaccine-based prevention of avian and swine-origin flu. Some new developments and concepts in diagnosis, (molecular) epidemiology, and therapy of AIDS, viral hepatitis C, and herpesvirus-associated diseases are outlined. Regulation of immune system has been discussed in a special issue 2010 including some aspects of CNS affections (measles). Mycobacterial infection and its prevention by modern recombinant vaccines have reached new interest, as well as new concepts of vaccination and prophylaxis against several other bacteria. Adaptation to host niches enables immune escape (example brucella) and determines virulence (example N. meningitidis). Chlamydia pneumoniae, previously considered to trigger atherosclerosis, is hypothetically associated to Alzheimer disease, while CMV, another putative trigger of atherosclerosis, gains evidence of oncomodulation in CNS tumor diseases. In terms of globalization, exotic virus infections are increasingly imported from southern countries.

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Resemblance and divergence: the “new” members of the genus Bordetella

Cited by 38 publications

References 70 publications

Novel Multitarget Real-Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens

Novel Multitarget Real-Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens

An Improved Recombination-Based In Vivo Expression Technology-Like Reporter System Reveals Differential cyaA Gene Activation in Bordetella Species

Recent publications in medical microbiology and immunology: a retrospective

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